Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,099,870	T→C	T287A (ACT→GCT)	ansB ←	L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,099,870	0	T	C	100.0%	266.9 / NA	75	T287A (ACT→GCT)	ansB	L‑asparaginase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/44);  total (31/44)

AGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTA  >  NC_000913/3099736‑3100005                                                                                                                                       |                                                                                                                                        agCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCgg                                                                                                                                    >  1:343559/1‑140 (MQ=255) agCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCgg                                                                                                                                    >  2:713869/1‑140 (MQ=255)      gTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTc                                                                                                                               >  2:497828/1‑140 (MQ=255)           agcagAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCgcggc                                                                                                                          >  1:674194/1‑140 (MQ=255)           agcagAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCgcggc                                                                                                                          >  2:117950/1‑140 (MQ=255)             cagAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGcggcgg                                                                                                                        >  1:637465/1‑140 (MQ=255)             cagAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGcggcgg                                                                                                                        >  1:1060583/1‑140 (MQ=255)                 aCGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGc                                                                                                                    >  1:985641/1‑140 (MQ=255)                  cgcgcgCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGcc                                                                                                                   >  2:668224/1‑140 (MQ=255)                  cgcgcgCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGcc                                                                                                                   >  1:1152499/1‑140 (MQ=255)                     gcgcTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGc                                                                                                                >  1:677766/1‑140 (MQ=255)                        cTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCgtg                                                                                                             <  2:66897/140‑1 (MQ=255)                        cTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCgtg                                                                                                             <  2:829497/140‑1 (MQ=255)                        cTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCgtg                                                                                                             <  2:256156/140‑1 (MQ=255)                        cTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCgtg                                                                                                             <  2:881636/140‑1 (MQ=255)                          ttttGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTc                                                                                                           <  2:1085256/140‑1 (MQ=255)                               cGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAaca                                                                                                      <  2:1152499/140‑1 (MQ=255)                               cGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAaca                                                                                                      <  1:256034/140‑1 (MQ=255)                               cGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAaca                                                                                                      <  1:293755/140‑1 (MQ=255)                               cGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAaca                                                                                                      <  1:149608/140‑1 (MQ=255)                               cGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAaca                                                                                                      <  1:789535/140‑1 (MQ=255)                               cGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAaca                                                                                                      <  1:745037/140‑1 (MQ=255)                                gggTTCAGGGGGCCCGAGGCGGCGAAGCCGTATTTCGCATCATCCCCCTCCGCCTCCTGAGTGGTTGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAACGTGTCGAacac                                                                                                     <  2:674194/140‑1 (MQ=255)                                  gTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAg                                                                                                   <  1:310716/140‑1 (MQ=255)                                    tCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGAt                                                                                                 <  2:985641/140‑1 (MQ=255)                                            ccAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCCGCGGGTCGAAAACAAATTTATACAg                                                                                         >  2:566023/1‑140 (MQ=255)                                             cAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAgg                                                                                        <  2:343559/140‑1 (MQ=255)                                              agagGCGACGAAGCCGTCTTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTCTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGt                                                                                       <  2:1041969/140‑1 (MQ=255)                                              agagGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGt                                                                                       <  1:432966/140‑1 (MQ=255)                                              agagGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGt                                                                                       <  2:316443/140‑1 (MQ=255)                                              agagGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGt                                                                                       <  1:799108/140‑1 (MQ=255)                                                agGCGACGAAGCCGTATTTCACATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTg                                                                                     <  2:1083887/140‑1 (MQ=255)                                                  gCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGcc                                                                                   >  1:602391/1‑140 (MQ=255)                                                   cgacgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCg                                                                                  <  2:905101/140‑1 (MQ=255)                                                   cgacgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCg                                                                                  <  1:800031/140‑1 (MQ=255)                                                   cgacgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCg                                                                                  <  1:216971/140‑1 (MQ=255)                                                   cgacgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCg                                                                                  <  1:358700/140‑1 (MQ=255)                                                   cgacgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCg                                                                                  <  1:497828/140‑1 (MQ=255)                                                     acgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGcc                                                                                   <  2:612804/137‑1 (MQ=255)                                                     acgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGcc                                                                                   >  1:612804/1‑137 (MQ=255)                                                     acgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGtt                                                                                >  1:648590/1‑140 (MQ=255)                                                     acgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGtt                                                                                >  2:1132371/1‑140 (MQ=255)                                                     acgaAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGtt                                                                                >  2:136460/1‑140 (MQ=255)                                                             gTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCacac                                                                        <  2:602391/140‑1 (MQ=255)                                                                tttCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAg                                                                     <  2:1060583/140‑1 (MQ=255)                                                                  tCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAgcg                                                                   <  2:738662/140‑1 (MQ=255)                                                                       tcatcCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAAc                                                              <  1:1155720/140‑1 (MQ=255)                                                                        catcCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACg                                                             <  1:909786/140‑1 (MQ=255)                                                                         atcCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGa                                                            <  1:117950/140‑1 (MQ=255)                                                                          tcCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGAt                                                           >  1:751748/1‑140 (MQ=255)                                                                          tcCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGAt                                                           >  1:1085177/1‑140 (MQ=255)                                                                           ccACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATg                                                          <  1:566023/140‑1 (MQ=255)                                                                                 cGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCcatca                                                    <  1:344844/140‑1 (MQ=255)                                                                                     aTCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGc                                                <  1:964357/140‑1 (MQ=255)                                                                                     aTCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGc                                                <  1:713869/140‑1 (MQ=255)                                                                                     aTCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGc                                                <  2:751748/140‑1 (MQ=255)                                                                                           aGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGTGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCAt                                          >  2:395441/1‑140 (MQ=255)                                                                                                tAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTAcc                                     >  2:680178/1‑140 (MQ=255)                                                                                                tAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTAcc                                     >  2:194223/1‑140 (MQ=255)                                                                                                tAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTAcc                                     >  1:144078/1‑140 (MQ=255)                                                                                                   cgcCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCa                                    <  1:1130747/138‑1 (MQ=255)                                                                                                   cgcCCGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCa                                    >  2:1130747/1‑138 (MQ=255)                                                                                                     cccGTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGc                                >  1:119954/1‑140 (MQ=255)                                                                                                        gTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCttt                             >  1:719239/1‑140 (MQ=255)                                                                                                        gTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCttt                             <  2:1113682/140‑1 (MQ=255)                                                                                                        gTCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGGGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCttt                             >  1:37016/1‑140 (MQ=255)                                                                                                         tCGGTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGGTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTa                            >  1:274094/1‑140 (MQ=255)                                                                                                           ggTACGCGGGAAGAACGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGc                          >  1:178494/1‑140 (MQ=255)                                                                                                                       gaaCGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGa              <  2:253810/140‑1 (MQ=255)                                                                                                                       gaaCGCACGACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGa              <  2:144078/140‑1 (MQ=255)                                                                                                                               gACTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAg      >  1:658829/1‑140 (MQ=255)                                                                                                                                 cTGCAGCACCGGTTTTCGCGGTGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCg    <  1:395441/140‑1 (MQ=255)                                                                                                                                 cTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCg    <  2:658829/140‑1 (MQ=255)                                                                                                                                 cTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCg    <  2:452126/140‑1 (MQ=255)                                                                                                                                 cTGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCg    <  1:106787/140‑1 (MQ=255)                                                                                                                                  tGCAGCACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGt   >  2:679412/1‑140 (MQ=255)                                                                                                                                   gcagcaCCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTa  <  1:668224/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTA  >  NC_000913/3099736‑3100005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑