Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,197,894	T→C	N583S (AAC→AGC)	glnE ←	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,197,894	0	T	C	100.0%	143.2 / NA	41	N583S (AAC→AGC)	glnE	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/22);  total (19/22)

ATGCGTAACAGCTGCGCCTGTTTGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGTTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAGGTGGT  >  NC_000913/3197759‑3198027                                                                                                                                        |                                                                                                                                      aTGCGTAACAGCTGCGCCTGTTTGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTgg                                                                                                                                   >  1:154332/1‑140 (MQ=255)      tAACAGCTGCGCCTGTTTGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGa                                                                                                                              >  1:560261/1‑140 (MQ=255)            cTGCGCCTGTTTGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAAtt                                                                                                                        >  1:194967/1‑140 (MQ=255)               cgcCTGTTTGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCAt                                                                                                                     >  1:394583/1‑140 (MQ=255)                gcCTGTTTGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGCAAAGGGTGCTTGGATCGAGCAATTCATc                                                                                                                    >  1:96315/1‑140 (MQ=255)                   tGTTTGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAg                                                                                                                 <  2:394583/140‑1 (MQ=255)                   tGTTTGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAg                                                                                                                 <  2:560261/140‑1 (MQ=255)                      ttGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCAAGCAATTCATCCAGCaa                                                                                                              <  2:1080855/140‑1 (MQ=255)                                         cAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAgc                                                                                           >  2:713723/1‑140 (MQ=255)                                                      ttCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAgctgg                                                                                        <  2:170358/130‑1 (MQ=255)                                                      ttCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAgctgg                                                                                        >  1:170358/1‑130 (MQ=255)                                                        cGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAatcatc                                                                            >  2:748283/1‑140 (MQ=255)                                                          tCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCgg                                                                          >  1:721960/1‑140 (MQ=255)                                                            aTCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCg                                                                        <  2:721960/140‑1 (MQ=255)                                                            aTCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCg                                                                        <  2:194967/140‑1 (MQ=255)                                                              cTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGAc                                                                      <  1:835490/140‑1 (MQ=255)                                                                tCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGAcgc                                                                    <  2:96315/140‑1 (MQ=255)                                                                  cGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCgg                                                                  <  1:608556/140‑1 (MQ=255)                                                                      aCGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGcaca                                                              <  2:94222/140‑1 (MQ=255)                                                                         cgcAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACag                                                           >  2:452600/1‑140 (MQ=255)                                                                            agcaAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAgaga                                                        >  2:458858/1‑140 (MQ=255)                                                                                  tACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCaa                                                  >  1:821988/1‑140 (MQ=255)                                                                                      ggCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGt                                              >  2:808123/1‑140 (MQ=255)                                                                                      ggCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGt                                              >  1:275690/1‑140 (MQ=255)                                                                                        cgcAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGttt                                            >  2:479022/1‑140 (MQ=255)                                                                                        cgcAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGGAAAGGGCGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGttt                                            >  1:907881/1‑140 (MQ=255)                                                                                                 tCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGcgccgc                                   <  2:275690/140‑1 (MQ=255)                                                                                                 tCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGcgccgc                                   <  2:453234/140‑1 (MQ=255)                                                                                                 tCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGcgccgc                                   <  1:479022/140‑1 (MQ=255)                                                                                                           cATCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCa                         <  1:713723/140‑1 (MQ=255)                                                                                                            aTCGGTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCAc                        >  2:1141010/1‑140 (MQ=255)                                                                                                                gTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAg                    <  2:154332/140‑1 (MQ=255)                                                                                                                gTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAg                    <  1:1141010/140‑1 (MQ=255)                                                                                                                gTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAg                    <  1:829800/140‑1 (MQ=255)                                                                                                                gTCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAg                    <  1:452600/140‑1 (MQ=255)                                                                                                                 tCGCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGc                   >  1:1173449/1‑140 (MQ=255)                                                                                                                   gCCGTCGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCaa                 >  1:600090/1‑140 (MQ=255)                                                                                                                        cGGCTGGTAAAGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTa            <  1:458858/140‑1 (MQ=255)                                                                                                                                aaaGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAggtg    <  1:808123/140‑1 (MQ=255)                                                                                                                                  aGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAggtggt  <  1:748283/140‑1 (MQ=255)                                                                                                                                  aGGGTGCTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAggtggt  <  2:578682/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      ATGCGTAACAGCTGCGCCTGTTTGAACTGACGCAGCGCCTCAAGCTGTTGCTCTTCGTCATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCGGCTGGTAAAGGGTGTTTGGATCGAGCAATTCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAGGTGGT  >  NC_000913/3197759‑3198027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑