Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	136,457	(T)8→7	coding (8/867 nt)	speE ←	spermidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	136,450	0	T	.	100.0%	299.5 / NA	69	coding (15/867 nt)	speE	spermidine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (28/41);  total (28/41)

CGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGT  >  NC_000913/136311‑136580                                                                                                                                            |                                                                                                                                   cGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGt                                                                                                                                    >  1:486117/1‑140 (MQ=255) cGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGt                                                                                                                                    >  2:525946/1‑140 (MQ=255)   ccATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGttt                                                                                                                                  >  1:869194/1‑140 (MQ=255)       ccAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAACCTCGTCGTGTAGCCTTTCATGCCACTGtttcttc                                                                                                                              <  2:957685/140‑2 (MQ=255)           cgcCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtttttttcggc                                                                                                                          >  1:1053872/1‑136 (MQ=255)              cATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCAt                                                                                                                      >  2:439717/1‑140 (MQ=255)              cATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCAt                                                                                                                      <  2:1053872/140‑1 (MQ=255)              cATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCAt                                                                                                                      >  2:15893/1‑140 (MQ=255)              cATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCAt                                                                                                                      <  1:690882/140‑1 (MQ=255)                 tACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATggg                                                                                                                   <  2:833770/140‑1 (MQ=255)                     cgACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGa                                                                                                               <  2:723888/140‑1 (MQ=255)                       aCCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATa                                                                                                             <  1:15893/140‑1 (MQ=255)                       aCCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATa                                                                                                             <  1:932010/140‑1 (MQ=255)                           aaTGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctc                                                                                                         <  2:282437/140‑1 (MQ=255)                                  cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGtt                                                                                                  >  2:117636/1‑140 (MQ=255)                                  cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGtt                                                                                                  >  2:1082281/1‑140 (MQ=255)                                    ttCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTaa                                                                                                <  2:119027/140‑1 (MQ=255)                                      ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                       >  2:59591/1‑131 (MQ=255)                                      ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                       <  1:59591/131‑1 (MQ=255)                                          aaaaTGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCg                                                                                          <  2:486117/140‑1 (MQ=255)                                                   aGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAg                                                                                 >  1:349619/1‑140 (MQ=255)                                                     aTCCTGGTGATCGGTCTTTTCATGTTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAggg                                                                               <  1:608738/140‑1 (MQ=255)                                                     aTCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGAAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAggg                                                                               <  1:525946/140‑1 (MQ=255)                                                       ccTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                             <  1:878948/140‑1 (MQ=255)                                                       ccTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                             <  1:19964/140‑1 (MQ=255)                                                       ccTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGGAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                             <  1:630226/140‑1 (MQ=255)                                                          ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCaa                                                                          >  1:56829/1‑140 (MQ=255)                                                          ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCaa                                                                          >  1:131464/1‑140 (MQ=255)                                                          ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCaa                                                                          <  2:855412/140‑1 (MQ=255)                                                          ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCaa                                                                          <  2:869194/140‑1 (MQ=255)                                                          ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCaa                                                                          >  1:428405/1‑140 (MQ=255)                                                             gATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTTGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACat                                                                       <  1:105759/140‑1 (MQ=255)                                                             gATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACat                                                                       <  2:131464/140‑1 (MQ=255)                                                              aTCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                                      <  1:1029936/140‑1 (MQ=255)                                                              aTCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                                      <  1:1082281/140‑1 (MQ=255)                                                              aTCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                                      <  1:210020/140‑1 (MQ=255)                                                              aTCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                                      <  1:1001163/140‑1 (MQ=255)                                                               tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACataa                                                                     >  1:76407/1‑140 (MQ=255)                                                                 ggTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACataata                                                                   >  2:735658/1‑140 (MQ=255)                                                                 ggTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACataata                                                                   >  1:218488/1‑140 (MQ=255)                                                                        tCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACa                                                            <  1:117636/140‑1 (MQ=255)                                                                             aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                                      >  1:500683/1‑125 (MQ=255)                                                                             aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                                      <  2:500683/125‑1 (MQ=255)                                                                             aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGAc                                                       >  2:256987/1‑140 (MQ=255)                                                                                 aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgcg                                                   >  1:222183/1‑140 (MQ=255)                                                                                 aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTAGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgcg                                                   >  1:498370/1‑140 (MQ=255)                                                                                   aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGa                                                 >  2:1087550/1‑140 (MQ=255)                                                                                    aCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgcg                                                   >  2:945142/1‑137 (MQ=255)                                                                                    aCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgcg                                                   <  1:945142/137‑1 (MQ=255)                                                                                      gTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGc                                              <  1:439717/140‑1 (MQ=255)                                                                                       ttATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                             <  2:674633/140‑1 (MQ=255)                                                                                       ttATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                             <  1:735658/140‑1 (MQ=255)                                                                                       ttATCTACCGCAAAGTACTGCCCAAACTGGTAGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                             <  2:498370/140‑1 (MQ=255)                                                                                          tCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACgg                                          >  1:842088/1‑140 (MQ=255)                                                                                                gCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAata                                    <  1:716096/140‑1 (MQ=255)                                                                                                 cAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                                        <  1:701164/135‑1 (MQ=255)                                                                                                 cAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                                        >  2:701164/1‑135 (MQ=255)                                                                                                           cccAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaaa                         >  2:495710/1‑140 (MQ=255)                                                                                                                  ttgTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAACTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATc                  <  2:272734/138‑1 (MQ=255)                                                                                                                  tGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATc                  <  1:1087550/140‑1 (MQ=255)                                                                                                                     tCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaaaa                        <  1:1071388/131‑1 (MQ=255)                                                                                                                     tCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaaaa                        >  2:1071388/1‑131 (MQ=255)                                                                                                                     tCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAgg               >  2:790956/1‑140 (MQ=255)                                                                                                                     tCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTGGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAgg               >  2:161611/1‑140 (MQ=255)                                                                                                                     gcGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAgg               <  2:595191/139‑1 (MQ=255)                                                                                                                      cGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAggg              <  1:495710/140‑1 (MQ=255)                                                                                                                      cGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAggg              <  2:428405/140‑1 (MQ=255)                                                                                                                              gTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTg      >  2:234851/1‑140 (MQ=255)                                                                                                                              gTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTg      >  1:133939/1‑140 (MQ=255)                                                                                                                               tttCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGa     <  1:256987/140‑1 (MQ=255)                                                                                                                                  cATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGt  <  2:76407/140‑1 (MQ=255)                                                                                                                                  cATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGt  >  1:438833/1‑140 (MQ=255)                                                                                                                                  cATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCATAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt        >  2:527302/1‑134 (MQ=255)                                                                                                                                  cATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCATAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt        <  1:527302/134‑1 (MQ=255)                                                                                                                                            |                                                                                                                                   CGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGT  >  NC_000913/136311‑136580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑