Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,267,921	(A)5→4	coding (93/987 nt)	yeiR →	zinc‑binding GTPase YeiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,267,917	0	A	.	100.0%	331.1 / NA	76	coding (89/987 nt)	yeiR	zinc‑binding GTPase YeiR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (39/37);  total (39/37)

AGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAG  >  NC_000913/2267785‑2268051                                                                                                                                     |                                                                                                                                       aGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaatggg                                                                                                                                 <  2:199906/140‑5 (MQ=255) aGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaatggg                                                                                                                                 <  1:549425/140‑5 (MQ=255) aGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaatggg                                                                                                                                 <  2:612501/140‑5 (MQ=255) aGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaatggg                                                                                                                                 <  1:841218/140‑5 (MQ=255)        aCGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaa                                                                                                                                      <  2:768742/128‑1 (MQ=255)        aCGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaa                                                                                                                                      >  1:768742/1‑128 (MQ=255)         cGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCGTAAAGATCCCAACG‑AAAATGGGCGGTACTg                                                                                                                        >  2:675466/1‑140 (MQ=255)             cAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAACTGGGCGGTACTGGATa                                                                                                                    >  2:1076334/1‑140 (MQ=255)             cAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTa                                                                                                                           <  2:138895/133‑1 (MQ=255)             cAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTa                                                                                                                           >  1:138895/1‑133 (MQ=255)              aGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTaa                                                                                                                   <  1:212535/140‑1 (MQ=255)              aGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTaa                                                                                                                   >  2:366744/1‑140 (MQ=255)                    cTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTAGTTAATGAAtt                                                                                                             >  1:169379/1‑140 (MQ=255)                        ccAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTggg                                                                                                         >  2:845268/1‑140 (MQ=255)                          agagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGa                                                                                                       >  1:978118/1‑140 (MQ=255)                          agagTATTGCGGAGCCATTGTGACCAGGACCAACCTCATCACCGGGTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGa                                                                                                       >  2:758988/1‑140 (MQ=255)                              tATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTc                                                                                                   >  1:556515/1‑140 (MQ=255)                                 tGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGa                                                                                                >  1:1064611/1‑140 (MQ=255)                                 tGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGa                                                                                                >  1:74719/1‑140 (MQ=255)                                 tGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGa                                                                                                >  2:754295/1‑140 (MQ=255)                                 tGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACAACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGa                                                                                                >  1:997840/1‑140 (MQ=255)                                   cGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAAt                                                                                              <  2:405608/140‑1 (MQ=255)                                   cGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAAt                                                                                              <  1:704493/140‑1 (MQ=255)                                   cGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAAt                                                                                              <  2:556515/140‑1 (MQ=255)                                      aGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGa                                                                                           >  1:295647/1‑140 (MQ=255)                                           ttGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTg                                                                                      >  1:439850/1‑140 (MQ=255)                                             gtgACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCgg                                                                                                 <  1:246309/127‑1 (MQ=255)                                             gtgACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCt                                                                                    <  1:437762/140‑1 (MQ=255)                                             gtgACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCt                                                                                    >  2:437762/1‑140 (MQ=255)                                             gtgACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCAACTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCgg                                                                                                 >  2:246309/1‑127 (MQ=255)                                                     gACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCTGAATTGATGGTGCTTTGCTCGc                                                                            >  1:394280/1‑140 (MQ=255)                                                     gACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGc                                                                            >  2:350931/1‑140 (MQ=255)                                                          aCCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATa                                                                       >  2:957318/1‑140 (MQ=255)                                                          aCCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATa                                                                       >  1:918661/1‑140 (MQ=255)                                                               atcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggc                                                                  >  1:26362/1‑140 (MQ=255)                                                               atcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggc                                                                  >  2:683980/1‑140 (MQ=255)                                                               atcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggc                                                                  >  1:395153/1‑140 (MQ=255)                                                               atcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggc                                                                  >  2:856351/1‑140 (MQ=255)                                                               atcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggc                                                                  >  1:541453/1‑140 (MQ=255)                                                                tcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggcg                                                                 <  1:350931/140‑1 (MQ=255)                                                                tcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggcg                                                                 <  1:683980/140‑1 (MQ=255)                                                                   ccGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCTGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCAt                                                              <  2:394280/140‑1 (MQ=255)                                                                                   cGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATccc                                              >  1:443757/1‑140 (MQ=255)                                                                                     ggAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCcg                                            >  2:327918/1‑140 (MQ=255)                                                                                     ggAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCcg                                            <  1:490922/140‑1 (MQ=255)                                                                                          aCCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCggcggc                                       <  1:957318/140‑1 (MQ=255)                                                                                             aCGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGc                                    <  1:856351/140‑1 (MQ=255)                                                                                             aCGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGc                                    <  1:327918/140‑1 (MQ=255)                                                                                             aCGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGc                                    <  2:443757/140‑1 (MQ=255)                                                                                                  gATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCAtgtg                               <  2:295647/140‑1 (MQ=255)                                                                                                   aTTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGtgc                              <  2:26362/140‑1 (MQ=255)                                                                                                   aTTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGtgc                              <  1:754295/140‑1 (MQ=255)                                                                                                    ttcttcATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGtgct                             <  1:957470/140‑1 (MQ=255)                                                                                                      cttcATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGtgctgc                           >  1:852447/1‑140 (MQ=255)                                                                                                         cATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGtt                        <  2:318762/140‑1 (MQ=255)                                                                                                           tCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTaa                      <  1:1085679/140‑1 (MQ=255)                                                                                                               ttAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGt                  >  2:617521/1‑140 (MQ=255)                                                                                                                 aGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCACTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGttt                >  2:475309/1‑140 (MQ=255)                                                                                                                  gCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTa               <  1:845268/140‑1 (MQ=255)                                                                                                                  gCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTa               <  1:758988/140‑1 (MQ=255)                                                                                                                   cccATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCTATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTAc              <  1:366744/140‑1 (MQ=255)                                                                                                                   cccATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTAc              <  1:959733/140‑1 (MQ=255)                                                                                                                     cATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTAccc            <  2:1064611/140‑1 (MQ=255)                                                                                                                      aTAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCa           >  1:22817/1‑140 (MQ=255)                                                                                                                       tAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAAt                     <  2:337733/129‑1 (MQ=255)                                                                                                                       tAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAAt                     >  1:337733/1‑129 (MQ=255)                                                                                                                       tAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAAt                     >  1:518277/1‑129 (MQ=255)                                                                                                                       tAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAAt                     <  2:518277/129‑1 (MQ=255)                                                                                                                       tAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCAt          <  2:22817/140‑1 (MQ=255)                                                                                                                        aaaGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGtt                        <  2:37615/125‑1 (MQ=255)                                                                                                                        aaaGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGtt                        >  1:37615/1‑125 (MQ=255)                                                                                                                        aaaGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATg         >  2:613352/1‑140 (MQ=255)                                                                                                                             tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGttt                >  2:1093057/1‑128 (MQ=255)                                                                                                                             tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGttt                >  2:61238/1‑128 (MQ=255)                                                                                                                             tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGttt                <  1:61238/128‑1 (MQ=255)                                                                                                                             tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGttt                <  1:1093057/128‑1 (MQ=255)                                                                                                                             tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGt    >  2:1057871/1‑140 (MQ=255)                                                                                                                             tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGt    >  2:133214/1‑140 (MQ=255)                                                                                                                             tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCACTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGt    <  1:475309/140‑1 (MQ=255)                                                                                                                              cccAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTa   <  1:675466/140‑1 (MQ=255)                                                                                                                              cccAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTa   <  2:918661/140‑1 (MQ=255)                                                                                                                               ccAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAg  <  2:74719/140‑1 (MQ=255)                                                                                                                               ccAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAg  <  2:997840/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       AGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAG  >  NC_000913/2267785‑2268051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑