Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,904	(A)8→7	coding (273/1539 nt)	emrY ←	tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,897	0	A	.	100.0%	220.0 / NA	51	coding (280/1539 nt)	emrY	tripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (31/20);  total (31/20)

CTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTC  >  NC_000913/2481758‑2482031                                                                                                                                            |                                                                                                                                       cTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGa                                                                                                                                        >  2:285515/1‑140 (MQ=255)                   ttCCTAAAAAAAAACCTCTGTAACAGTGAAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGt                                                                                                                     <  1:34984/140‑1 (MQ=255)                                   tctGTGACAGTGAAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAAtt                                                                                                     <  1:729781/140‑1 (MQ=255)                                     tgtgACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                                                                                                   >  2:192804/1‑140 (MQ=255)                                     tgtgACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                                                                                                   >  1:350195/1‑140 (MQ=255)                                      gtgACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc                                                                                                  >  1:545413/1‑140 (MQ=255)                                       tgACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGcc                                                                                                 >  1:968126/1‑140 (MQ=255)                                            gTGGAATTAACGGCCCC‑CCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCt                                                                                           <  1:647059/140‑1 (MQ=255)                                             tGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTCAATTCGCCTATTCt                                                                                           >  1:557376/1‑140 (MQ=255)                                                 aTTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgt                                                                                       >  2:140209/1‑140 (MQ=255)                                                         ccccGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGcc                                                                                    <  2:1048926/135‑1 (MQ=255)                                                         ccccGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGcc                                                                                    >  1:1048926/1‑135 (MQ=255)                                                         ccccGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCt                                                                               <  2:1016839/140‑1 (MQ=255)                                                           ccGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGc                                                                             <  2:230256/140‑1 (MQ=255)                                                            cGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGcc                                                                            >  1:831138/1‑140 (MQ=255)                                                            cGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGcc                                                                            >  2:467777/1‑140 (MQ=255)                                                                   aaCCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACa                                                                     <  1:140209/140‑1 (MQ=255)                                                                        cTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGAt                                                                <  1:192804/140‑1 (MQ=255)                                                                           aaCGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGc                                                             >  2:1085994/1‑140 (MQ=255)                                                                                ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                                                        >  1:1004860/1‑140 (MQ=255)                                                                                ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                                                        >  2:955724/1‑140 (MQ=255)                                                                                ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                                                        >  1:980146/1‑140 (MQ=255)                                                                                ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                                                        >  1:622350/1‑140 (MQ=255)                                                                                ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                                                        >  1:1042737/1‑140 (MQ=255)                                                                                ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                                                        >  2:981596/1‑140 (MQ=255)                                                                                ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                                                        >  2:934834/1‑140 (MQ=255)                                                                                ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                                                        >  1:585284/1‑140 (MQ=255)                                                                                 tctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGc                                                       <  2:490559/140‑1 (MQ=255)                                                                                  ctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCa                                                      <  1:981596/140‑1 (MQ=255)                                                                                       aaaTATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGc                                                 <  1:999070/140‑1 (MQ=255)                                                                                          tatCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGc                                                       >  1:1049561/1‑131 (MQ=255)                                                                                          tatCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGc                                                       <  2:1049561/131‑1 (MQ=255)                                                                                           atCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTaca                                             <  1:454924/140‑1 (MQ=255)                                                                                           atCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTaca                                             >  1:947565/1‑140 (MQ=255)                                                                                                 acaTCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAAc                                       <  2:186620/140‑1 (MQ=255)                                                                                                   aTCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGa                                     <  2:1004860/140‑1 (MQ=255)                                                                                                   aTCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGa                                     <  1:1085994/140‑1 (MQ=255)                                                                                                   aTCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGa                                     <  1:285515/140‑1 (MQ=255)                                                                                                     cAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAgg                                   >  1:977632/1‑140 (MQ=255)                                                                                                             gTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGa                                >  1:1032267/1‑135 (MQ=255)                                                                                                             gTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGa                                <  2:1032267/135‑1 (MQ=255)                                                                                                                       tACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCtt                 >  1:894647/1‑140 (MQ=255)                                                                                                                       tACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCtt                 >  1:636197/1‑140 (MQ=255)                                                                                                                              tAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGcc                   <  2:138399/131‑1 (MQ=255)                                                                                                                              tAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGcc                   >  1:138399/1‑131 (MQ=255)                                                                                                                              tAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGt          >  2:426914/1‑140 (MQ=255)                                                                                                                               aaTGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGtt         >  1:898559/1‑140 (MQ=255)                                                                                                                               aaTGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGtt         >  1:844039/1‑140 (MQ=255)                                                                                                                               aaTGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGtt         >  1:354688/1‑140 (MQ=255)                                                                                                                                aTGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTg        >  1:578081/1‑140 (MQ=255)                                                                                                                                 tGAAGACAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGa       <  2:898559/140‑1 (MQ=255)                                                                                                                                      aCAGCG‑AAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCAGTTGATGCTc  >  1:758007/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       CTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTC  >  NC_000913/2481758‑2482031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑