| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,682,157 | (C)7→8 | coding (589/3282 nt) | yphG ← | DUF5107 domain‑containing protein YphG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,682,150 | 1 | . | C | 97.6% | 144.8 / ‑3.2 | 41 | coding (596/3282 nt) | yphG | DUF5107 domain‑containing protein YphG |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (25/15); total (25/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
CGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGT > NC_000913/2682018‑2682278 | cGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑ccccc‑cc > 2:852942/1‑140 (MQ=255) cGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCC‑CCTTTCACTGCCGGGTTgg > 1:681234/1‑140 (MQ=255) cGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGc > 1:878376/1‑140 (MQ=255) tagtCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCccacc > 1:790552/1‑140 (MQ=255) gtCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCcaccac > 1:928377/1‑140 (MQ=255) tCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACcaca > 2:464666/1‑140 (MQ=255) tGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATg > 1:406974/1‑140 (MQ=255) tagtaAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGCTGGCCCACCACAAGAAATGAc > 2:53394/1‑140 (MQ=255) agtaAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAcg > 1:908559/1‑140 (MQ=255) aGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCCGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAccccgc > 2:761690/1‑135 (MQ=255) gcgggTAGCGAGGGGAAAGGGGGGGGCGGCCCGTTTCCCGTGACCAAACCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg < 2:445331/137‑1 (MQ=255) gCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg > 1:445331/1‑140 (MQ=255) gCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg > 2:170230/1‑140 (MQ=255) tggggagggggAAGGGGGAGACGGCCCTTTTGCGGTAATAAACCACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt < 1:316159/133‑1 (MQ=255) tAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt > 2:741813/1‑140 (MQ=255) tAGAGAGGGGAAAAGCGGAGACGGCCCTTTCGCCGTAATAAACCACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt < 1:758243/140‑1 (MQ=255) gggggggggggAAGGCGGAGCCGCCCCTTTCCCGGTGACCAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt < 2:688831/133‑1 (MQ=255) aGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTc > 2:866455/1‑140 (MQ=255) gCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTcc > 2:190851/1‑140 (MQ=255) aTGGGGAAAGCGGAGACGGCCCGTTTACCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGt > 2:266557/1‑140 (MQ=255) gCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGa > 2:571888/1‑140 (MQ=255) gCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGa > 2:766293/1‑140 (MQ=255) cccccgccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTc < 2:673395/137‑1 (MQ=255) gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc > 1:253560/1‑140 (MQ=255) gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc > 1:521733/1‑140 (MQ=255) gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGGTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc > 2:1013980/1‑140 (MQ=255) ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg > 2:471950/1‑140 (MQ=255) ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg > 2:1029867/1‑140 (MQ=255) ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg < 1:950724/140‑1 (MQ=255) ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg > 1:179093/1‑140 (MQ=255) cgTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCCGCCTTTCACGGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc < 2:436846/140‑1 (MQ=255) tCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGa < 2:928377/140‑1 (MQ=255) tCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGa < 2:1003435/140‑1 (MQ=255) cggcggGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGaaa < 1:471950/140‑1 (MQ=255) ggcggGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAAc > 2:418721/1‑140 (MQ=255) ggcggGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAAc < 2:253560/140‑1 (MQ=255) aaGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtca > 1:520216/1‑140 (MQ=255) aGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat < 2:179093/140‑1 (MQ=255) aGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat < 1:495329/140‑1 (MQ=255) aGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat < 1:418721/140‑1 (MQ=255) tctGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTg < 2:878376/140‑1 (MQ=255) tctGATGCCCTT‑‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCAGCGTGGCGTTCCCGTTATCGACGCGGCTGGCGATTTCCAGCACCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGt < 2:385082/140‑1 (MQ=255) ctGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGt < 1:190851/140‑1 (MQ=255) | CGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGT > NC_000913/2682018‑2682278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |