Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	319,490	A→G	V171A (GTT→GCT)	rclA ←	putative pyridine nucleotide‑disulfide oxidoreductase RclA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	319,490	0	A	G	100.0%	333.2 / NA	90	V171A (GTT→GCT)	rclA	putative pyridine nucleotide‑disulfide oxidoreductase RclA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/47);  total (43/47)

TAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAACGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTTGTGCACCGGT  >  NC_000913/319353‑319623                                                                                                                                          |                                                                                                                                      taataTCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCg                                                                                                                                     <  1:312056/140‑1 (MQ=255)   atatCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGcc                                                                                                                                   <  2:265261/140‑1 (MQ=255)    tatCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGcc                                                                                                                                   >  2:198922/1‑139 (MQ=255)    tatCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGcc                                                                                                                                   <  1:198922/139‑1 (MQ=255)       cGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAata                                                                                                                               >  1:651729/1‑140 (MQ=255)       cGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAata                                                                                                                               >  1:1033441/1‑140 (MQ=255)       cGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAata                                                                                                                               >  1:144447/1‑140 (MQ=255)         aCGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAatata                                                                                                                             >  2:1043169/1‑140 (MQ=255)         aCGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAatata                                                                                                                             >  1:91279/1‑140 (MQ=255)              cTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATccgc                                                                                                                        >  1:740923/1‑140 (MQ=255)               tGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATccgcc                                                                                                                       >  2:1027001/1‑140 (MQ=255)                gATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATccgccg                                                                                                                      >  1:430458/1‑140 (MQ=255)                 aTCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCcgccgc                                                                                                                     <  1:59896/140‑1 (MQ=255)                    gcgTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCa                                                                                                                  >  2:1025895/1‑140 (MQ=255)                       tAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCaaaa                                                                                                               <  2:740923/140‑1 (MQ=255)                           aTCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATAcc                                                                                                           >  2:811850/1‑140 (MQ=255)                               tcgCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTaaa                                                                                                       >  2:974248/1‑140 (MQ=255)                                          tCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCaa                                                                                            >  1:485958/1‑140 (MQ=255)                                               aaTATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtt                                                                                       >  2:313525/1‑140 (MQ=255)                                               aaTATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtt                                                                                       >  1:59556/1‑140 (MQ=255)                                               aaTATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtt                                                                                       <  1:194967/140‑1 (MQ=255)                                               aaTATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtt                                                                                       >  2:1064959/1‑140 (MQ=255)                                                 tatCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtttt                                                                                     >  1:332316/1‑140 (MQ=255)                                                 tatCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtttt                                                                                     >  2:565148/1‑140 (MQ=255)                                                 tatCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtttt                                                                                     >  2:51753/1‑140 (MQ=255)                                                 tatCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtttt                                                                                     >  2:229872/1‑140 (MQ=255)                                                 tatCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtttt                                                                                     >  2:1093673/1‑140 (MQ=255)                                                 tatCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCtttt                                                                                     >  1:1084642/1‑140 (MQ=255)                                                   tCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCa                                                                                             <  1:1068482/130‑1 (MQ=255)                                                   tCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCa                                                                                             >  2:1068482/1‑130 (MQ=255)                                                   tCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAg                                                                                   >  1:196783/1‑140 (MQ=255)                                                    cccGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCTAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGa                                                                                  <  1:32660/140‑1 (MQ=255)                                                    cccGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGa                                                                                  >  2:850749/1‑140 (MQ=255)                                                      cGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGAtt                                                                                <  2:332316/140‑1 (MQ=255)                                                        aTCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGa                                                                                  <  1:394508/136‑1 (MQ=255)                                                        aTCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGa                                                                                  >  2:394508/1‑136 (MQ=255)                                                        aTCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTaa                                                                              >  2:775317/1‑140 (MQ=255)                                                        aTCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTaa                                                                              >  2:98468/1‑140 (MQ=255)                                                         tCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAg                                                                             <  1:845895/140‑1 (MQ=255)                                                             cccGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAAt                                                                         >  2:646133/1‑140 (MQ=255)                                                             cccGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAAt                                                                         >  2:821205/1‑140 (MQ=255)                                                                gAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCg                                                                      <  1:1064959/140‑1 (MQ=255)                                                                 aGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCgg                                                                     <  1:350661/140‑1 (MQ=255)                                                                 aGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCgg                                                                     <  1:850749/140‑1 (MQ=255)                                                                 aGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCgg                                                                     <  2:651729/140‑1 (MQ=255)                                                                 aGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCgg                                                                     <  2:1033441/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATTCCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  2:729260/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  2:430458/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  1:974248/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  2:196783/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  2:1084642/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  1:51753/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  1:1027001/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  1:161137/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  1:229872/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  1:313525/140‑1 (MQ=255)                                                                   gCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  1:646133/140‑1 (MQ=255)                                                                     aaaaaCAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCt                                                                 <  1:1025895/140‑1 (MQ=255)                                                                      aaaaCAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTg                                                                <  1:821205/140‑1 (MQ=255)                                                                       aaaCAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   <  1:259982/136‑1 (MQ=255)                                                                       aaaCAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTg                                                                   >  2:259982/1‑136 (MQ=255)                                                                           aGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCata                                                           <  2:91279/140‑1 (MQ=255)                                                                           aGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCata                                                           <  1:1043169/140‑1 (MQ=255)                                                                           aGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCata                                                           <  2:370729/140‑1 (MQ=255)                                                                           aGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCata                                                           <  2:298090/140‑1 (MQ=255)                                                                            gCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCatat                                                          >  1:855948/1‑140 (MQ=255)                                                                             cGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCAtata                                                         <  2:876379/140‑1 (MQ=255)                                                                                 gctgctTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTcc                                                     <  2:1003297/140‑1 (MQ=255)                                                                                 gctgctTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTcc                                                     <  2:485958/140‑1 (MQ=255)                                                                                             aTGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTggtggt                                         <  1:565148/140‑1 (MQ=255)                                                                                             aTGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTggtggt                                         <  2:851356/140‑1 (MQ=255)                                                                                                 ttACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAAtt                                     >  1:315588/1‑140 (MQ=255)                                                                                                 ttACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAAtt                                     >  2:261686/1‑140 (MQ=255)                                                                                                         ctgcCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAAt                             >  1:69314/1‑140 (MQ=255)                                                                                                          tgcCAAAATTAGCGAACATAGAGGCGAACTCAGCGCCAATATATCCGCAGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAATAATCCGGTGCTGTCATATACTCCTGGCGTAGTGGTAATTCCAGGAAtt                            >  2:1053061/1‑140 (MQ=255)                                                                                                                      cGAACATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACg                <  1:1030351/140‑1 (MQ=255)                                                                                                                          cATAGAGGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGttt            >  2:1084589/1‑140 (MQ=255)                                                                                                                               agGCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTTGTGCa       <  1:1084589/140‑1 (MQ=255)                                                                                                                                ggCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTTGTGCAc      <  1:771846/140‑1 (MQ=255)                                                                                                                                 gCGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTTGTGCGcc     >  1:714140/1‑140 (MQ=255)                                                                                                                                  cGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAAcc                   <  1:835684/125‑1 (MQ=255)                                                                                                                                  cGAACTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAAcc                   >  2:835684/1‑125 (MQ=255)                                                                                                                                    aaCTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTtg           >  1:348877/1‑131 (MQ=255)                                                                                                                                    aaCTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTtg           <  2:348877/131‑1 (MQ=255)                                                                                                                                    aaCTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTTGTGCa       <  1:888699/135‑1 (MQ=255)                                                                                                                                    aaCTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTTGTGCa       <  1:378667/135‑1 (MQ=255)                                                                                                                                    aaCTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTTGTGCa       >  2:888699/1‑135 (MQ=255)                                                                                                                                    aaCTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTTGTGCa       >  2:378667/1‑135 (MQ=255)                                                                                                                                    aaCTCAGCGCCAATATATCCGCCACCCAAAATACCTAAAAGCCCAGGCAGTTCTATTAGATTAAGTGATGCGGTGATGTCGTATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGAAACCAAGACGTGTGCACCgga  >  2:730820/1‑139 (MQ=255)                                                                                                                                     aCTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTtgt          >  1:178491/1‑131 (MQ=255)                                                                                                                                     aCTCAGCGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTtgt          <  2:178491/131‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGCGAACTCAACGCCAATATATCCGCCGCCCAAAATACCTAAATGCCCAGGCAATTCTTTTAGATTAAGTAATCCGGTGCTGTCATATACTCCTGGCGTGGTGGTAATTCCAGGAATTGGCGGAACCACGGTTTGTGCACCGGT  >  NC_000913/319353‑319623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑