Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,115,807	A→G	R472R (CGT→CGC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,115,807	0	A	G	100.0%	190.7 / NA	52	R472R (CGT→CGC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/23);  total (29/23)

TACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCG  >  NC_000913/4115676‑4115942                                                                                                                                    |                                                                                                                                        tACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTc                                                                                                                                 >  1:214600/1‑140 (MQ=255) tACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTc                                                                                                                                 >  2:575216/1‑140 (MQ=255)   ccGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTcgc                                                                                                                               >  2:758106/1‑140 (MQ=255)   ccGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTcgc                                                                                                                               >  2:632786/1‑140 (MQ=255)   ccGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTcgc                                                                                                                               >  2:154959/1‑140 (MQ=255)   ccGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTcgc                                                                                                                               >  1:387332/1‑140 (MQ=255)        tGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCa                                                                                                                          <  1:154959/140‑1 (MQ=255)           ggCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATc                                                                                                                       <  2:972079/140‑1 (MQ=255)             cATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCa                                                                                                                      <  2:731708/139‑1 (MQ=255)             cATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCa                                                                                                                      >  1:731708/1‑139 (MQ=255)             cATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCAc                                                                                                                     >  2:333136/1‑140 (MQ=255)               tAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCg                                                                                                                   <  2:538184/140‑1 (MQ=255)               tAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCg                                                                                                                   <  2:493517/140‑1 (MQ=255)                 aaCGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCt                                                                                                                 >  1:226788/1‑140 (MQ=255)                 aaCGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCt                                                                                                                 <  1:453966/140‑1 (MQ=255)                           tCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCa                                                                                                       <  1:80018/140‑1 (MQ=255)                                            cgtcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGc                                                                                      >  1:5433/1‑140 (MQ=255)                                            cgtcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGc                                                                                      <  1:758106/140‑1 (MQ=255)                                            cgtcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGc                                                                                      >  1:940288/1‑140 (MQ=255)                                              tcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCtcgtc                                                                                                >  2:91345/1‑128 (MQ=255)                                              tcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCtcgtc                                                                                                <  1:91345/128‑1 (MQ=255)                                               cgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGc                                                                                                     >  1:431372/1‑122 (MQ=255)                                               cgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGc                                                                                                     <  2:431372/122‑1 (MQ=255)                                                  gtTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAg                                                                                >  2:532838/1‑140 (MQ=255)                                                     cttcCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCa                                                                             <  2:940288/140‑1 (MQ=255)                                                           aCGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGcc                                                                       >  1:675717/1‑140 (MQ=255)                                                             gCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCCCCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCa                                                                             <  2:394331/132‑1 (MQ=255)                                                             gCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCa                                                                             >  1:394331/1‑132 (MQ=255)                                                              ccATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAgg                                                                    <  1:575216/140‑1 (MQ=255)                                                                aTCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGTGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGcc                                                                  >  1:331583/1‑140 (MQ=255)                                                                  cgcgcgTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTg                                                                >  2:389650/1‑140 (MQ=255)                                                                  cgcgcgTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTg                                                                >  1:617017/1‑140 (MQ=255)                                                                  cgcgcgTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTg                                                                <  2:617017/140‑1 (MQ=255)                                                                  cgcgcgTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATAACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTg                                                                >  1:342485/1‑140 (MQ=255)                                                                       gTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATAACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCgaga                                                           <  2:342485/140‑1 (MQ=255)                                                                        tttAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGAt                                                          <  2:675717/140‑1 (MQ=255)                                                                        tttAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGAt                                                          <  2:5433/140‑1 (MQ=255)                                                                            aCCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGc                                                      <  1:632786/140‑1 (MQ=255)                                                                                   ttttCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCAccc                                               >  2:13994/1‑140 (MQ=255)                                                                                     ttCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCaa                                             <  1:389650/140‑1 (MQ=255)                                                                                          gCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCaa                                             >  2:904014/1‑135 (MQ=255)                                                                                          gCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCaa                                             <  1:904014/135‑1 (MQ=255)                                                                                               cGTAACGGTAATTACGCTCAGTGGTTTCGATGCTTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACt                                   <  2:85377/140‑1 (MQ=255)                                                                                                             cGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCCCCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCgg                     <  2:214600/140‑1 (MQ=255)                                                                                                                 cAGTGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGgcgc                 >  2:673795/1‑140 (MQ=255)                                                                                                                    tGGTTTCGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCa              >  2:441615/1‑140 (MQ=255)                                                                                                                       tttCGATGCCTGGGCGGACCTCGCGCTCAATCACCCCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAAcg           <  1:128584/140‑1 (MQ=255)                                                                                                                          cGATGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCgg        >  2:635418/1‑140 (MQ=255)                                                                                                                            aTGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGt      <  1:304403/140‑1 (MQ=255)                                                                                                                            aTGCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGt      >  1:211306/1‑140 (MQ=255)                                                                                                                              gCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGc    >  2:811620/1‑140 (MQ=255)                                                                                                                              gCCTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGc    >  2:85159/1‑140 (MQ=255)                                                                                                                                cTGGGCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGGTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCg  >  2:98103/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        TACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCG  >  NC_000913/4115676‑4115942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑