Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,462,837	T→C	intergenic (‑177/+217)	nrdD ← / ← treC	anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,462,837	0	T	C	100.0%	146.3 / NA	39	intergenic (‑177/+217)	nrdD/treC	anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/27);  total (12/27)

GTTTTCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAAT  >  NC_000913/4462711‑4462970                                                                                                                               |                                                                                                                                      gTTTTCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCAtttt                                                                                                                          <  1:956814/140‑1 (MQ=255)   tttCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTaa                                                                                                                        <  1:111/140‑1 (MQ=255)   tttCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTaa                                                                                                                        <  1:156733/140‑1 (MQ=255)   tttCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTaa                                                                                                                        <  2:201217/140‑1 (MQ=255)   tttCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTaa                                                                                                                        <  1:994385/140‑1 (MQ=255)   tttCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTaa                                                                                                                        <  1:913551/140‑1 (MQ=255)   tttCCAGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTaa                                                                                                                        <  2:644287/140‑1 (MQ=255)     tCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAg                                                                                                                      <  2:213204/140‑1 (MQ=255)     tCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAg                                                                                                                      <  2:106657/140‑1 (MQ=255)     tCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAg                                                                                                                      <  1:957518/140‑1 (MQ=255)     tCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAg                                                                                                                      <  1:853529/140‑1 (MQ=255)     tCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAg                                                                                                                      <  1:714098/140‑1 (MQ=255)        ggTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCt                                                                                                                   <  1:976052/140‑1 (MQ=255)        ggTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCt                                                                                                                   <  1:334921/140‑1 (MQ=255)        ggTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGCAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCt                                                                                                                   <  1:445250/140‑1 (MQ=255)          ttttttCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCtat                                                                                                                 <  2:324681/140‑1 (MQ=255)          ttttttCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCtat                                                                                                                 <  2:423645/140‑1 (MQ=255)                     cgtttTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCtt                                                                                                      <  1:799350/140‑1 (MQ=255)                     cgtttTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCtt                                                                                                      <  2:406979/140‑1 (MQ=255)                                 cTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGAt                                                                                          >  2:579743/1‑140 (MQ=255)                                         aaTCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGc                                                                                  >  2:298148/1‑140 (MQ=255)                                              ccATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGc                                                                                  >  2:554589/1‑135 (MQ=255)                                              ccATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGc                                                                                  <  1:554589/135‑1 (MQ=255)                                                  ccGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCt                                                                                 <  1:1064626/132‑1 (MQ=255)                                                  ccGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCt                                                                                 >  2:1064626/1‑132 (MQ=255)                                                                cgccgcACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTa                                                           <  2:105000/140‑1 (MQ=255)                                                                   cgcACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTaaaa                                                        >  2:202201/1‑140 (MQ=255)                                                                       ccTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCa                                                    <  1:298148/140‑1 (MQ=255)                                                                         tttttCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCaga                                                  <  2:903955/140‑1 (MQ=255)                                                                           tttCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGc                                                >  1:643039/1‑140 (MQ=255)                                                                                  gggAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGa                                         >  1:302885/1‑140 (MQ=255)                                                                                    gAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATg                                       <  2:643039/140‑1 (MQ=255)                                                                                       cTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACg                                    >  2:915151/1‑140 (MQ=255)                                                                                         gtagtaGCAATTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACggg                                  <  1:202201/140‑1 (MQ=255)                                                                                                  aTTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGgcgc                               <  1:932624/134‑1 (MQ=255)                                                                                                  aTTATAGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGgcgc                               >  2:932624/1‑134 (MQ=255)                                                                                                    tataGTCGATTAATACAACATATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGa                       >  2:506905/1‑140 (MQ=255)                                                                                                                      caTATTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATg     >  1:318619/1‑140 (MQ=255)                                                                                                                         atTGGGCTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAAt  >  1:535155/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                      GTTTTCCGGTTTTTTCGTTTCGTTTTCCGGTGCTTTCCGCAATCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAAT  >  NC_000913/4462711‑4462970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑