Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,591,161	A→G	V40A (GTG→GCG)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,591,161	0	A	G	100.0%	351.2 / NA	93	V40A (GTG→GCG)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/46);  total (47/46)

GGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGCATGTA  >  NC_000913/4591025‑4591297                                                                                                                                         |                                                                                                                                         ggTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGa                                                                                                                                       >  1:691295/1‑140 (MQ=255) ggTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACAGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGa                                                                                                                                       >  2:960762/1‑140 (MQ=255)  gTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGAt                                                                                                                                      >  1:691057/1‑140 (MQ=255)    cGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATcc                                                                                                                                    >  2:359097/1‑140 (MQ=255)    cGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATcc                                                                                                                                    >  1:380620/1‑140 (MQ=255)          gTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCAGCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCTCCAGATACACCGATACAGAGGCGACCGCGATCCACAggg                                                                                                                              <  2:342990/140‑1 (MQ=255)            aGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGgcg                                                                                                                            >  2:535016/1‑140 (MQ=255)            aGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGgcg                                                                                                                            >  1:608680/1‑140 (MQ=255)            aGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGgcg                                                                                                                            >  2:413958/1‑140 (MQ=255)                 aGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGAt                                                                                                                       >  2:127719/1‑140 (MQ=255)                     cgtcgtTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGc                                                                                                                   >  1:58664/1‑140 (MQ=255)                      gtcgtTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCt                                                                                                                  <  1:535016/140‑1 (MQ=255)                      gtcgtTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCt                                                                                                                  <  1:925197/140‑1 (MQ=255)                      gtcgtTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCt                                                                                                                  <  2:587418/140‑1 (MQ=255)                           tgttAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTccccc                                                                                                             <  1:413958/140‑1 (MQ=255)                               aataaCCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACGGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGAc                                                                                                         <  2:587384/140‑1 (MQ=255)                                       cAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCa                                                                                                 >  1:282642/1‑140 (MQ=255)                                       cAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCa                                                                                                 >  1:613033/1‑140 (MQ=255)                                              cgcgcgcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACcg                                                                                          <  2:644923/140‑1 (MQ=255)                                              cgcgcgcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACcg                                                                                          <  1:960762/140‑1 (MQ=255)                                                  cgcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCCCCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAg                                                                                      <  1:275059/140‑1 (MQ=255)                                                  cgcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAg                                                                                      <  1:842743/140‑1 (MQ=255)                                                                   catcaCCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCgatga                                                                     >  2:121277/1‑140 (MQ=255)                                                                     tcaCCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGAtt                                                                   >  2:302392/1‑140 (MQ=255)                                                                     tcaCCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGAtt                                                                   >  2:1062850/1‑140 (MQ=255)                                                                       aCCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTcc                                                                 <  1:1062850/140‑1 (MQ=255)                                                                         cTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCga                                                               >  2:511226/1‑140 (MQ=255)                                                                         cTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCga                                                               >  2:279160/1‑140 (MQ=255)                                                                           tCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCgaga                                                             <  2:691295/140‑1 (MQ=255)                                                                           tCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCgaga                                                             <  1:359097/140‑1 (MQ=255)                                                                           tCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCgaga                                                             <  2:753373/140‑1 (MQ=255)                                                                           tCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCgaga                                                             <  1:127719/140‑1 (MQ=255)                                                                            cTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            <  2:691057/140‑1 (MQ=255)                                                                            cTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            <  1:1068462/140‑1 (MQ=255)                                                                            cTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            <  1:335442/140‑1 (MQ=255)                                                                            cTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            >  1:906765/1‑140 (MQ=255)                                                                            cTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            <  2:282642/140‑1 (MQ=255)                                                                            cTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            >  1:737897/1‑140 (MQ=255)                                                                            cTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            <  1:121277/140‑1 (MQ=255)                                                                            cTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            >  2:32368/1‑140 (MQ=255)                                                                             tGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAAt                                                           <  2:380620/140‑1 (MQ=255)                                                                               ggCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCCCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCa                                                         <  2:522229/140‑1 (MQ=255)                                                                                 cGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCAcc                                                       <  1:254278/140‑1 (MQ=255)                                                                                   aTGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCAc                                                        <  1:886962/137‑1 (MQ=255)                                                                                   aTGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCAc                                                        >  2:886962/1‑137 (MQ=255)                                                                                   aTGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCa                                                     <  2:906765/140‑1 (MQ=255)                                                                                   aTGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCa                                                     <  1:279160/140‑1 (MQ=255)                                                                                         aGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTAt                                               >  1:1018139/1‑140 (MQ=255)                                                                                              gtagtaGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            >  1:663510/1‑122 (MQ=255)                                                                                              gtagtaGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGaa                                                            <  2:663510/122‑1 (MQ=255)                                                                                              gtagtaGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCt                                          >  1:962086/1‑140 (MQ=255)                                                                                              gtagtaGCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCt                                          <  2:329875/140‑1 (MQ=255)                                                                                               tagtagCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCtt                                         >  2:725452/1‑140 (MQ=255)                                                                                               tagtagCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCtt                                         >  1:1091154/1‑140 (MQ=255)                                                                                                agtagCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTg                                        >  1:479597/1‑140 (MQ=255)                                                                                                agtagCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTg                                        >  1:284632/1‑140 (MQ=255)                                                                                                  tagCGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGaa                                      <  1:302392/140‑1 (MQ=255)                                                                                                     cGATACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAatat                                   >  2:62090/1‑140 (MQ=255)                                                                                                       aTACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCt                                 >  1:492632/1‑140 (MQ=255)                                                                                                       aTACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCt                                 >  2:182704/1‑140 (MQ=255)                                                                                                       aTACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCt                                 >  2:330607/1‑140 (MQ=255)                                                                                                       aTACGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCt                                 >  1:684918/1‑140 (MQ=255)                                                                                                         aCGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCttt                               >  2:106698/1‑140 (MQ=255)                                                                                                         aCGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCttt                               >  1:662044/1‑140 (MQ=255)                                                                                                         aCGCCACCAGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCttt                               >  2:332156/1‑140 (MQ=255)                                                                                                            ccaccaGATACACCGATACAGAGGCGACCGCGATCCAGAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGATTCACCCAGGGTATGTGCTTGAATATCTTTTTa                            >  2:973219/1‑140 (MQ=255)                                                                                                            ccaccaGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTa                            <  1:542280/140‑1 (MQ=255)                                                                                                              accaGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGt                          <  2:479597/140‑1 (MQ=255)                                                                                                              accaGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGt                          <  1:32368/140‑1 (MQ=255)                                                                                                                 aGATACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATc                       <  1:106698/140‑1 (MQ=255)                                                                                                                   aTACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCa                     <  1:330607/140‑1 (MQ=255)                                                                                                                   aTACACCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCa                     <  1:62090/140‑1 (MQ=255)                                                                                                                      cacCGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAg                  >  1:548867/1‑140 (MQ=255)                                                                                                                         cGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTaa               <  1:725452/140‑1 (MQ=255)                                                                                                                         cGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTaa               >  2:554985/1‑140 (MQ=255)                                                                                                                         cGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTaa               >  1:360144/1‑140 (MQ=255)                                                                                                                         cGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTaa               <  1:554985/140‑1 (MQ=255)                                                                                                                         cGATACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTaa               >  2:639777/1‑140 (MQ=255)                                                                                                                           aTACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTaaaa             >  1:1083294/1‑140 (MQ=255)                                                                                                                           aTACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTaaaa             >  1:677497/1‑140 (MQ=255)                                                                                                                            tACAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAAc            >  2:439199/1‑140 (MQ=255)                                                                                                                             aCAGAGGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAAcc           >  2:175572/1‑140 (MQ=255)                                                                                                                               agagGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTg         <  2:677497/140‑1 (MQ=255)                                                                                                                                 agGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGc       <  2:662044/140‑1 (MQ=255)                                                                                                                                 agGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGc       <  2:608680/140‑1 (MQ=255)                                                                                                                                 agGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGc       <  1:511226/140‑1 (MQ=255)                                                                                                                                 agGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGc       <  1:439199/140‑1 (MQ=255)                                                                                                                                 agGCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGc       >  2:75227/1‑140 (MQ=255)                                                                                                                                  ggCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGCa      <  2:1091154/140‑1 (MQ=255)                                                                                                                                   gCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAAc            <  2:1042092/133‑1 (MQ=255)                                                                                                                                   gCGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAAc            >  1:1042092/1‑133 (MQ=255)                                                                                                                                    cGACCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGCATg    <  2:492632/140‑1 (MQ=255)                                                                                                                                      aCCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGCATGTa  <  2:58664/140‑1 (MQ=255)                                                                                                                                      aCCGCGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGCATGTa  <  2:367006/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAACTACCGCGAGGCAGAATGCACCGATGATTCCGAGAATCACCCAGGGTATGTGCTTGAATATCTTTTTAGTATCCATAGTAAAACCTGGCATGTA  >  NC_000913/4591025‑4591297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑