Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	359,407	C→T	A203A (GCC→GCT)	cynT →	carbonic anhydrase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	359,407	0	C	T	100.0%	191.8 / NA	54	A203A (GCC→GCT)	cynT	carbonic anhydrase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/30);  total (24/30)

TAATTTGCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCCGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGCCATT  >  NC_000913/359272‑359542                                                                                                                                        |                                                                                                                                        tAATTTGCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGTTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTa                                                                                                                                     <  2:1053352/140‑1 (MQ=255) tAATTTGCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTa                                                                                                                                     >  2:377096/1‑140 (MQ=255) tAATTTGCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTa                                                                                                                                     <  1:47129/140‑1 (MQ=255)    tttGCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATc                                                                                                                                  >  2:180262/1‑140 (MQ=255)      tGCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCt                                                                                                                                >  2:362553/1‑140 (MQ=255)       gCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTc                                                                                                                               <  1:387554/140‑1 (MQ=255)        cAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTcg                                                                                                                              <  1:29029/140‑1 (MQ=255)           aCTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGt                                                                                                                           <  2:810694/140‑1 (MQ=255)           aCTCACCCACGGGTGCGCCGGGCGCTCGGAGGGGGGCGGATCGCCCTGCACGGGTGGGTCTACGACTTTGAAAGCGGCAGCATCGCAGCTTTTGCCGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGt                                                                                                                           <  2:236562/140‑1 (MQ=255)             tCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGttt                                                                                                                         >  1:426443/1‑140 (MQ=255)              catccatcGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTtg                                                                                                                        <  2:690550/140‑1 (MQ=255)              catccatcGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTtg                                                                                                                        <  1:180262/140‑1 (MQ=255)               atccatcGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACTGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTtgt                                                                                                                       >  2:63398/1‑140 (MQ=255)               atccatcGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTtgg                                                                                                                       >  2:755131/1‑139 (MQ=255)                tccatcGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTtgtg                                                                                                                      >  1:915617/1‑140 (MQ=255)                     cGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATa                                                                                                                 >  1:769148/1‑140 (MQ=255)                       gTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATAcc                                                                                                               >  1:873778/1‑140 (MQ=255)                       gTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATAcc                                                                                                               >  1:225587/1‑140 (MQ=255)                         gcgcCTGGCGCTCGAAGAGGAGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGc                                                                                                             >  2:136062/1‑140 (MQ=255)                              gggCGCTCGAAGGGGGGCGGTTCCCCCTGCCGGGCGGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGc                                                                                                        <  2:816523/139‑1 (MQ=255)                                        agagGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTGGACGGCGCACCCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAaccgaccg                                                                                              <  2:268952/140‑1 (MQ=255)                                           ggggCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAg                                                                                           >  2:805403/1‑140 (MQ=255)                                                  aTCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAAcc                                                                                    <  2:915617/140‑1 (MQ=255)                                                  aTCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAAcc                                                                                    >  2:516110/1‑140 (MQ=255)                                                             cGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCACCCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTaaa                                                                         <  2:77931/140‑1 (MQ=255)                                                                   ggTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCa                                                                   >  1:812914/1‑140 (MQ=255)                                                                   ggTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCGTATTTTTAAACCATCa                                                                   >  2:988139/1‑140 (MQ=255)                                                                    gTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAg                                                                  <  1:136062/140‑1 (MQ=255)                                                                     tCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAgg                                                                 >  2:1031342/1‑140 (MQ=255)                                                                         cGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTATTCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGtt                                                             <  2:769148/140‑1 (MQ=255)                                                                          gACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTc                                                            <  2:225587/140‑1 (MQ=255)                                                                               tGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAAcc                                                                       <  1:44514/124‑1 (MQ=255)                                                                               tGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAAcc                                                                       >  2:44514/1‑124 (MQ=255)                                                                               tGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTccacca                                                       >  1:288282/1‑140 (MQ=255)                                                                               tGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTccacca                                                       >  1:610897/1‑140 (MQ=255)                                                                               tGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTc                                                            >  1:475842/1‑135 (MQ=255)                                                                               tGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTc                                                            <  2:475842/135‑1 (MQ=255)                                                                                    gCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGAtt                                                  <  1:377096/140‑1 (MQ=255)                                                                                    gCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGAtt                                                  <  2:426443/140‑1 (MQ=255)                                                                                      ggCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCa                                                >  1:177711/1‑140 (MQ=255)                                                                                      ggCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCa                                                >  2:221930/1‑140 (MQ=255)                                                                                        cagcaTCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGt                                              >  1:475125/1‑140 (MQ=255)                                                                                              cGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACaaa                                        >  2:900821/1‑140 (MQ=255)                                                                                               gCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAAt                                       <  1:140142/140‑1 (MQ=255)                                                                                                  gCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTaa                                    <  1:900821/140‑1 (MQ=255)                                                                                                          cGGCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAata                            <  2:475125/140‑1 (MQ=255)                                                                                                           ggCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAatat                           <  2:288282/140‑1 (MQ=255)                                                                                                           ggCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAatat                           <  1:258417/140‑1 (MQ=255)                                                                                                           ggCGCAACCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAatat                           <  1:805403/140‑1 (MQ=255)                                                                                                                aaCCCGCCAGTTTGTGCCACTGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAATATTCGTc                      <  1:516110/140‑1 (MQ=255)                                                                                                                                    tGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGCCAtt  <  1:63398/140‑1 (MQ=255)                                                                                                                                    tGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGCCAtt  <  1:755131/140‑1 (MQ=255)                                                                                                                                    tGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGCCAtt  <  1:836537/140‑1 (MQ=255)                                                                                                                                    tGGCTGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGCCAtt  <  1:1031342/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TAATTTGCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCCGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAACCTTATTTTTAAACCATCAGGAGTTCCACCATGATTCAGTCACAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGCCATT  >  NC_000913/359272‑359542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑