Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,573	G→A	G720R (GGG→AGG)	ybbP →	putative ABC transporter membrane subunit YbbP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,573	0	G	A	100.0%	187.3 / NA	53	G720R (GGG→AGG)	ybbP	putative ABC transporter membrane subunit YbbP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/21);  total (32/21)

GAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGC  >  NC_000913/522438‑522708                                                                                                                                        |                                                                                                                                        gAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGaa                                                                                                                                     >  1:547428/1‑140 (MQ=255)      ggTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGTTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAAc                                                                                                                                >  2:975099/1‑140 (MQ=255)      ggTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAAc                                                                                                                                >  1:432847/1‑140 (MQ=255)          aGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAActgct                                                                                                                            <  2:945408/140‑1 (MQ=255)          aGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAActgct                                                                                                                            <  2:263445/140‑1 (MQ=255)          aGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAActgct                                                                                                                            <  2:77343/140‑1 (MQ=255)            tCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACtgctgc                                                                                                                          >  1:1082914/1‑140 (MQ=255)            tCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACtgctgc                                                                                                                          >  1:51662/1‑140 (MQ=255)                gcgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTAc                                                                                                                      <  1:130242/140‑1 (MQ=255)                 cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTAcc                                                                                                                     <  2:547428/140‑1 (MQ=255)                 cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTAcc                                                                                                                     >  2:229842/1‑140 (MQ=255)                 cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTAcc                                                                                                                     <  2:38628/140‑1 (MQ=255)                 cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTAcc                                                                                                                     <  2:432847/140‑1 (MQ=255)                  gcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCa                                                                                                                    >  1:1278/1‑140 (MQ=255)                     ggAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTAcc                                                                                                                     <  1:886240/136‑1 (MQ=255)                     ggAAGTGATGGGGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTAcc                                                                                                                     >  2:886240/1‑136 (MQ=255)                      gAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGtt                                                                                                                <  1:229842/140‑1 (MQ=255)                                    aCTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCg                                                                                                  <  2:542675/140‑1 (MQ=255)                                    aCTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCg                                                                                                  <  1:975099/140‑1 (MQ=255)                                     cTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGc                                                                                                 >  1:108327/1‑140 (MQ=255)                                        gTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCAt                                                                                              <  2:1278/140‑1 (MQ=255)                                        gTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAAATGCTGCGTACCACGTTGTGGTGTGAGTTCGCCAt                                                                                              >  1:921165/1‑140 (MQ=255)                                         tCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATg                                                                                             >  2:114383/1‑140 (MQ=255)                                          cACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGc                                                                                            >  2:746267/1‑140 (MQ=255)                                           aCCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCt                                                                                           <  1:605883/140‑1 (MQ=255)                                            ccGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCtt                                                                                          <  2:51662/140‑1 (MQ=255)                                               ccTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTggg                                                                                       >  1:971008/1‑140 (MQ=255)                                                 tGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGTTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGgtt                                                                                     <  2:1091611/140‑1 (MQ=255)                                                             ctgttgctgGCACAGGTGCCGGTGGGAATGCGTTAGCGTCATCAGGAGCTGGGGGTGTGGCGCACACTCGGTGCGAAGAAAAAACTGCTTCGTAACACGTTGTGGAGTGAGTTCGCCCAGCTTGGGTTTGTTTCCAGCCt                                                                         >  2:507855/1‑140 (MQ=255)                                                             ctgttgctgGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCt                                                                         >  1:925726/1‑140 (MQ=255)                                                               gttgctgGCACAGGTGCAGGAGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCtgg                                                                       >  2:325483/1‑140 (MQ=255)                                                                ttgctgGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCtggt                                                                      >  2:214787/1‑140 (MQ=255)                                                                ttgctgGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCtggt                                                                      <  1:214787/140‑1 (MQ=255)                                                                  gctgGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCtggtgg                                                                    >  2:809759/1‑140 (MQ=255)                                                                  gctgGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCtggtgg                                                                    >  1:603009/1‑140 (MQ=255)                                                                    tgGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGcc                                                                  >  2:117112/1‑140 (MQ=255)                                                                     ggCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCg                                                                 >  1:490341/1‑140 (MQ=255)                                                                     ggCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCg                                                                 >  2:22653/1‑140 (MQ=255)                                                                      gCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGc                                                                <  2:971008/140‑1 (MQ=255)                                                                       cacaGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCa                                                               >  1:15070/1‑140 (MQ=255)                                                                          aggtgcaggtgGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAAtt                                                            >  2:239610/1‑140 (MQ=255)                                                                                  gtgGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGa                                                    >  1:916524/1‑140 (MQ=255)                                                                                      gAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACg                                                >  2:320008/1‑140 (MQ=255)                                                                                       aaTGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACgg                                               >  1:798220/1‑140 (MQ=255)                                                                                          gcgtcagcgtcaTCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGgcgc                                            <  1:861374/140‑1 (MQ=255)                                                                                          gcgtcagcgtcaTCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGgcgc                                            >  2:849174/1‑140 (MQ=255)                                                                                           cgtcagcgtcaTCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCt                                           <  1:114383/140‑1 (MQ=255)                                                                                             tcagcgtcaTCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCTgg                                         <  2:798220/140‑1 (MQ=255)                                                                                             tcagcgtcaTCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCTgg                                         >  2:688607/1‑140 (MQ=255)                                                                                                     atcaGGAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTg                                 >  1:78659/1‑140 (MQ=255)                                                                                                          gAGCTGGTGGTGTGGCGCACACTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGc                            >  2:1048628/1‑140 (MQ=255)                                                                                                                           cacaCTCGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTc           >  2:605887/1‑140 (MQ=255)                                                                                                                                 cGGTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTggg     <  1:45975/140‑1 (MQ=255)                                                                                                                                  ggTGCGAGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGa    >  1:48738/1‑140 (MQ=255)                                                                                                                                    tGCGAGGAAAAAACTGCTGCGTTCCATGTTGTGGTGTGAGTTGGCCATGCTTGGGTTTGTGTTCGGGCTGGTGGTCGCAATTGGTGCGGAAACGGCGCTGGGAATGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGc  <  2:420918/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGCCTGGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGATTTTCCGTGGGAGC  >  NC_000913/522438‑522708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑