Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	530,589	A→G	V213A (GTG→GCG)	selU ←	tRNA 2‑selenouridine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	530,589	0	A	G	100.0%	144.3 / NA	39	V213A (GTG→GCG)	selU	tRNA 2‑selenouridine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

CTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCA  >  NC_000913/530459‑530724                                                                                                                                   |                                                                                                                                        ctctTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGgc                                                                                                                                >  2:321472/1‑140 (MQ=255) ctctTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGgc                                                                                                                                >  1:578879/1‑140 (MQ=255)      cGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCca                                                                                                                                     <  1:1073463/130‑1 (MQ=255)      cGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCca                                                                                                                                     >  2:1073463/1‑130 (MQ=255)            ggCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGa                                                                                                                     <  1:223474/140‑1 (MQ=255)                cTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACggg                                                                                                                 >  2:1000369/1‑140 (MQ=255)                cTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACggg                                                                                                                 >  2:505618/1‑140 (MQ=255)                 tCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGc                                                                                                                >  1:735182/1‑140 (MQ=255)                  cAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCg                                                                                                               <  2:73458/140‑1 (MQ=255)                   aaGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGt                                                                                                              <  1:1000369/140‑1 (MQ=255)                               aaaCGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCa                                                                                                  >  1:321239/1‑140 (MQ=255)                                         tctACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGcagc                                                                                        >  2:830161/1‑140 (MQ=255)                                             ccaccGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGcagccagc                                                                                    >  2:528123/1‑140 (MQ=255)                                                 cGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGt                                                                                >  2:45949/1‑140 (MQ=255)                                                   cAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGttt                                                                              >  1:882225/1‑140 (MQ=255)                                                               gAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGcc                                                                  <  1:45949/140‑1 (MQ=255)                                                                     ttCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTa                                                            <  2:882225/140‑1 (MQ=255)                                                                       cgctcgcGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAg                                                          <  2:578879/140‑1 (MQ=255)                                                                          tcgcGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTtg                                                       <  1:157522/140‑1 (MQ=255)                                                                            gcgcAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTtgtg                                                     <  2:614751/140‑1 (MQ=255)                                                                             cgcAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTgg                                                    >  1:331762/1‑140 (MQ=255)                                                                                    aTTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAAc                                             >  1:283556/1‑140 (MQ=255)                                                                                       ccGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTg                                          <  1:528123/140‑1 (MQ=255)                                                                                            aGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGAcc                                     >  2:934888/1‑140 (MQ=255)                                                                                            aGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGAcc                                     <  1:797989/140‑1 (MQ=255)                                                                                                    cGAACCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCg                             <  1:776509/140‑1 (MQ=255)                                                                                                      aaCCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAATGCCGaa                           <  2:283556/140‑1 (MQ=255)                                                                                                       aCCGATCATCCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAAc                          <  2:735182/140‑1 (MQ=255)                                                                                                           atcatcCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACcgcg                      >  2:315800/1‑140 (MQ=255)                                                                                                           atcatcCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACcgcg                      >  1:615610/1‑140 (MQ=255)                                                                                                            tcatcCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGa                     >  2:214293/1‑140 (MQ=255)                                                                                                               tcCGGCTTTCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGa                  <  2:321239/140‑1 (MQ=255)                                                                                                                     tttCGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGcc            >  2:785732/1‑140 (MQ=255)                                                                                                                        cGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAAc         <  2:331762/140‑1 (MQ=255)                                                                                                                        cGTCTTCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAAc         <  1:830161/140‑1 (MQ=255)                                                                                                                            ttCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCtt     <  1:505618/140‑1 (MQ=255)                                                                                                                             tCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTc    <  2:728812/140‑1 (MQ=255)                                                                                                                             tCCAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTc    <  1:701332/140‑1 (MQ=255)                                                                                                                               cAGCGCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCa  <  1:337122/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        CTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCA  >  NC_000913/530459‑530724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑