Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	593,034	A→G	L49L (TTG→CTG)	nfrB ←	bacteriophage N4 receptor, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	593,034	0	A	G	100.0%	135.0 / NA	37	L49L (TTG→CTG)	nfrB	bacteriophage N4 receptor, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/17);  total (20/17)

GTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAACTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCGTAGAGCCAGGTAGCAAAAACATCAA  >  NC_000913/592898‑593165                                                                                                                                         |                                                                                                                                    gTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCtt                                                                                                                                  <  1:1019574/140‑1 (MQ=255)     tCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGt                                                                                                                              >  1:438055/1‑140 (MQ=255)        ccgccAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTa                                                                                                                           >  2:191546/1‑140 (MQ=255)             aGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATgcg                                                                                                                      <  1:191546/140‑1 (MQ=255)               cTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATgcggc                                                                                                                    >  1:366877/1‑140 (MQ=255)                 cGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGt                                                                                                                  <  1:91132/140‑1 (MQ=255)                    ccATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTAcc                                                                                                               <  1:387014/140‑1 (MQ=255)                        atTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGt                                                                                                                  >  2:949930/1‑133 (MQ=255)                        atTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGt                                                                                                                  <  1:949930/133‑1 (MQ=255)                                                    gCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAg                                                                               >  2:691163/1‑140 (MQ=255)                                                        gAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCg                                                                           <  2:438055/140‑1 (MQ=255)                                                                   gCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACat                                                                >  1:708722/1‑140 (MQ=255)                                                                    cTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACatg                                                               >  2:633278/1‑140 (MQ=255)                                                                        cGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACAtgatga                                                           <  1:95657/140‑1 (MQ=255)                                                                                     ggTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGttgt                                              <  2:600207/140‑3 (MQ=255)                                                                                           taCAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGAt                                        <  1:345486/140‑1 (MQ=255)                                                                                           taCAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGAt                                        >  1:1049610/1‑140 (MQ=255)                                                                                           taCAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGAt                                        <  1:929265/140‑1 (MQ=255)                                                                                                ttCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACtt                                   >  2:414837/1‑140 (MQ=255)                                                                                                ttCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACtt                                   >  2:668953/1‑140 (MQ=255)                                                                                                ttCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACtt                                   >  1:1043312/1‑140 (MQ=255)                                                                                                ttCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACtt                                   <  2:708722/140‑1 (MQ=255)                                                                                                ttCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACtt                                   >  2:85699/1‑140 (MQ=255)                                                                                                ttCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACtt                                   >  1:664853/1‑140 (MQ=255)                                                                                                ttCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACtt                                   >  1:38423/1‑140 (MQ=255)                                                                                                ttCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACtt                                   >  1:313571/1‑140 (MQ=255)                                                                                                 tCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACttt                                  >  2:948694/1‑140 (MQ=255)                                                                                                 tCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACttt                                  >  1:174084/1‑140 (MQ=255)                                                                                                   gcgGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTa                                <  1:691163/140‑1 (MQ=255)                                                                                                        aaCTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCg                           <  2:215538/140‑1 (MQ=255)                                                                                                          cTCATTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCGTa                         >  1:400048/1‑140 (MQ=255)                                                                                                             aTTCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCGTagag                      <  2:664853/140‑1 (MQ=255)                                                                                                               tCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCGTAGAGcc                    >  2:29189/1‑140 (MQ=255)                                                                                                               tCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCGTAGAGcc                    <  2:400048/140‑1 (MQ=255)                                                                                                               tCGCGGGTAGCGGCGATAAACACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCGTAGAGcc                    >  1:741259/1‑140 (MQ=255)                                                                                                                                 aaCACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCGTAGAGCCAGGTAGCAAAAACATCaa  <  2:366877/140‑1 (MQ=255)                                                                                                                                 aaCACTCAGCTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCGTAGAGCCAGGTAGCAAAAACATCaa  <  1:948694/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACCATAATCGCTAACGGTTTTTCATCTGGTTTATACAGTTCGCGGTAACTCATTCGCGGGTAGCGGCGATAAACACTCAACTTGCGTTTAATGCGGCGTACCCAGTAGACGACATCAATAAAAAAATCGTCCAGCCCGCTGATGAACATGATGACCGCTAACGTTATCGCGATTACTTTTAAGCCGTAGAGCCAGGTAGCAAAAACATCAA  >  NC_000913/592898‑593165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑