Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	648,427	A→G	V382A (GTG→GCG)	citF ←	citrate lyase alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	648,427	0	A	G	100.0%	179.8 / NA	49	V382A (GTG→GCG)	citF	citrate lyase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/27);  total (22/27)

GGTCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTACGGGCC  >  NC_000913/648291‑648561                                                                                                                                         |                                                                                                                                       ggTCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGt                                                                                                                                     <  2:133400/140‑1 (MQ=255)  gTCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGtt                                                                                                                                    >  2:101294/1‑140 (MQ=255)       tACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGt                                                                                                                               >  1:868096/1‑140 (MQ=255)       tACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGt                                                                                                                               >  1:430405/1‑140 (MQ=255)           ttATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGaa                                                                                                                           <  1:951362/140‑1 (MQ=255)           ttATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGaa                                                                                                                           <  1:784654/140‑1 (MQ=255)            tATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAAc                                                                                                                          >  2:932715/1‑140 (MQ=255)            tATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAAc                                                                                                                          >  2:863889/1‑140 (MQ=255)              tCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTg                                                                                                                        >  1:158735/1‑140 (MQ=255)              tCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTg                                                                                                                        >  1:123560/1‑140 (MQ=255)                 accaGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGTGCGTTAACGTTGAACTgggc                                                                                                                     >  2:558773/1‑139 (MQ=255)                 accaGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTgggc                                                                                                                     >  2:492962/1‑139 (MQ=255)                              tACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAgc                                                                                                        <  1:602594/140‑1 (MQ=255)                               aCGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAgcg                                                                                                       <  1:581225/140‑1 (MQ=255)                                cGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAgcgc                                                                                                      >  2:495567/1‑140 (MQ=255)                                  aCCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAgcgcgc                                                                                                    <  1:1081035/140‑1 (MQ=255)                                  aCCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAgcgcgc                                                                                                    <  1:932715/140‑1 (MQ=255)                                   ccGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCt                                                                                                   <  1:495567/140‑1 (MQ=255)                                   ccGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCt                                                                                                   <  2:514358/140‑1 (MQ=255)                                   ccGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCt                                                                                                   >  2:309240/1‑140 (MQ=255)                                   ccGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCt                                                                                                   <  2:140164/140‑1 (MQ=255)                                    cgcgTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCt                                                                                                   <  1:1011534/139‑1 (MQ=255)                                    cgcgTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCt                                                                                                   >  2:1011534/1‑139 (MQ=255)                                      cgTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAg                                                                                                <  1:948840/140‑1 (MQ=255)                                      cgTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAg                                                                                                >  1:1021515/1‑140 (MQ=255)                                                gcgcGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACg                                                                                      >  2:898186/1‑140 (MQ=255)                                                 cgcgACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGt                                                                                     >  2:1060732/1‑140 (MQ=255)                                                      cgaTGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCgaga                                                                                <  1:863889/140‑1 (MQ=255)                                                        atgatgGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGGCGAGACg                                                                              <  2:713219/140‑1 (MQ=255)                                                           atgGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATc                                                                           <  2:158735/140‑1 (MQ=255)                                                                   cgcAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATg                                                                   <  1:552656/140‑1 (MQ=255)                                                                   cgcAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATg                                                                   >  2:522007/1‑140 (MQ=255)                                                                   cgcAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATg                                                                   >  2:552656/1‑140 (MQ=255)                                                                     cAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCGGTACCACCACGTCGAGACGATCAACCGATgcg                                                                 <  2:236949/140‑1 (MQ=255)                                                                           cAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTc                                                           <  1:492962/140‑1 (MQ=255)                                                                               cgcgGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAAc                                                       <  1:101294/140‑1 (MQ=255)                                                                                  ggTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAAcccc                                                    <  1:1060732/140‑1 (MQ=255)                                                                                  ggTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAAcccc                                                    <  2:868096/140‑1 (MQ=255)                                                                                               ccaccGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTg                                       <  2:579741/140‑1 (MQ=255)                                                                                                   cGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTg                                   <  1:898186/140‑1 (MQ=255)                                                                                                       aGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGgcgc                               <  2:123560/140‑1 (MQ=255)                                                                                                       aGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGgcgc                               <  1:536899/140‑1 (MQ=255)                                                                                                                       ccGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGAtt               <  1:427897/140‑1 (MQ=255)                                                                                                                       ccGTCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAACTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGAtt               <  2:1021515/140‑1 (MQ=255)                                                                                                                          tCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTggg            >  1:652628/1‑140 (MQ=255)                                                                                                                          tCAGAGCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTggg            >  2:454808/1‑140 (MQ=255)                                                                                                                             gagCCGGTCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGtt         >  2:755664/1‑140 (MQ=255)                                                                                                                                    tCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTACGGGcc  >  1:126614/1‑140 (MQ=255)                                                                                                                                    tCAGCGCGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTACGGGcc  >  1:414343/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GGTCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTATCGCAGTGACCACCGGAAGCACCACGCAGTACGCCGTCAGAGCCGGTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTACGGGCC  >  NC_000913/648291‑648561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑