Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	994,115	C→T	V240I (GTC→ATC)	ssuC ←	aliphatic sulfonate ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	994,115	0	C	T	100.0%	240.3 / NA	68	V240I (GTC→ATC)	ssuC	aliphatic sulfonate ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/30);  total (38/30)

CGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGT  >  NC_000913/993981‑994250                                                                                                                                       |                                                                                                                                        cgTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAg                                                                                                                                    >  2:945061/1‑140 (MQ=255)      tGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGt                                                                                                                               >  2:92501/1‑140 (MQ=255)           gCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGcc                                                                                                                          >  2:1008401/1‑140 (MQ=255)                 tGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGc                                                                                                                      <  1:83703/138‑1 (MQ=255)                 tGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGc                                                                                                                      >  2:83703/1‑138 (MQ=255)                 tGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAg                                                                                                                    >  2:227742/1‑140 (MQ=255)                     ttGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCg                                                                                                                <  2:32578/140‑1 (MQ=255)                     ttGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCg                                                                                                                <  1:675263/140‑1 (MQ=255)                      tGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGt                                                                                                               >  2:545192/1‑140 (MQ=255)                        aGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGt                                                                                                                               <  2:876388/122‑1 (MQ=255)                        aGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGt                                                                                                                               >  1:876388/1‑122 (MQ=255)                                 ggCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAataata                                                                                                    <  2:331427/140‑1 (MQ=255)                                   cGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGc                                                                                                  <  1:545192/140‑1 (MQ=255)                                     tGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCg                                                                                                                <  2:304674/124‑1 (MQ=255)                                     tGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCg                                                                                                                >  1:304674/1‑124 (MQ=255)                                       cccTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCTGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACt                                                                                              >  2:530570/1‑140 (MQ=255)                                       cccTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGaataa                                                                                                      >  1:405253/1‑132 (MQ=255)                                       cccTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGaataa                                                                                                      <  2:405253/132‑1 (MQ=255)                                       cccTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACt                                                                                              >  1:494107/1‑140 (MQ=255)                                       cccTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACt                                                                                              >  2:404040/1‑140 (MQ=255)                                       cccTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACt                                                                                              <  2:494107/140‑1 (MQ=255)                                            gTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAg                                                                                                           <  1:88792/122‑1 (MQ=255)                                            gTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAg                                                                                                           >  2:88792/1‑122 (MQ=255)                                            gTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTaccac                                                                                         >  1:671642/1‑140 (MQ=255)                                            gTTCAGACGAACAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTaccac                                                                                         >  1:543033/1‑140 (MQ=255)                                                 gACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGt                                                                                    <  2:1052286/140‑1 (MQ=255)                                                     aGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGt                                                                                <  2:10080/140‑1 (MQ=255)                                                          tATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCa                                                                           >  1:858419/1‑140 (MQ=255)                                                              cATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCaaaaa                                                                       <  1:981288/140‑1 (MQ=255)                                                                 aCCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGt                                                                                <  1:269855/128‑1 (MQ=255)                                                                 aCCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGt                                                                                >  2:269855/1‑128 (MQ=255)                                                                 aCCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTc                                                                    >  1:229692/1‑140 (MQ=255)                                                                    gTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCcg                                                                 >  1:846900/1‑140 (MQ=255)                                                                    gTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCcg                                                                 >  2:46079/1‑140 (MQ=255)                                                                    gTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCcg                                                                 >  2:629208/1‑140 (MQ=255)                                                                    gTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCcg                                                                 >  2:806900/1‑140 (MQ=255)                                                                     tGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCcgc                                                                >  2:514493/1‑140 (MQ=255)                                                                      ggCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCcgcg                                                               >  1:12027/1‑140 (MQ=255)                                                                          tcctTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCAtt                                                           <  2:671642/140‑1 (MQ=255)                                                                          tcctTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCAtt                                                           >  1:74763/1‑140 (MQ=255)                                                                          tcctTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCAtt                                                           <  1:806900/140‑1 (MQ=255)                                                                            ctTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCa                                                         >  1:718854/1‑140 (MQ=255)                                                                              tCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATc                                                       <  2:229692/140‑1 (MQ=255)                                                                                aaaTGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGc                                                     <  1:227742/140‑1 (MQ=255)                                                                                aaaTGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGc                                                     <  1:92501/140‑1 (MQ=255)                                                                                 aaTGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGcc                                                    >  2:722931/1‑140 (MQ=255)                                                                                    gATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAGTAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGa                                                 <  1:307850/140‑1 (MQ=255)                                                                                    gATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGa                                                 <  1:629208/140‑1 (MQ=255)                                                                                          ccGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCa                                           >  1:499401/1‑140 (MQ=255)                                                                                             ggTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATg                                        <  1:1072527/140‑1 (MQ=255)                                                                                              gTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATc                                                       >  1:642148/1‑124 (MQ=255)                                                                                              gTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATc                                                       <  2:642148/124‑1 (MQ=255)                                                                                               ttCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGcc                                      >  1:932614/1‑140 (MQ=255)                                                                                                   aGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGaa                                  >  2:101534/1‑140 (MQ=255)                                                                                                    gcgcAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAAt                                 >  1:200223/1‑140 (MQ=255)                                                                                                    gcgcAACCAGAGGCGCTCTAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAAt                                 <  1:530570/140‑1 (MQ=255)                                                                                                                   ctctAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGttt                  <  2:12027/140‑1 (MQ=255)                                                                                                                   ctctAACAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGttt                  >  2:888240/1‑140 (MQ=255)                                                                                                                       aaCAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGc              <  1:888240/140‑1 (MQ=255)                                                                                                                       aaCAGCTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGc              <  2:718854/140‑1 (MQ=255)                                                                                                                            cTGCGCGCTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGcaacaa         >  2:710668/1‑140 (MQ=255)                                                                                                                              gcgcgcTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGttt                  >  1:596176/1‑129 (MQ=255)                                                                                                                              gcgcgcTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGttt                  <  2:596176/129‑1 (MQ=255)                                                                                                                               cgcgcTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCa      >  1:456892/1‑140 (MQ=255)                                                                                                                                gcgcTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAg     >  2:516251/1‑140 (MQ=255)                                                                                                                                 cgcTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGc    >  2:296331/1‑140 (MQ=255)                                                                                                                                  gcTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCg   <  2:200223/140‑1 (MQ=255)                                                                                                                                   cTGATGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGt  <  1:722931/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTGCCGAGCAGGGCGTAAAGAATAATAGCGACTACCACCACGTCCGTTTGCAAAAACTCCCGCGCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGT  >  NC_000913/993981‑994250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑