Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,010,382	A→G	N140S (AAC→AGC)	uup →	ATP‑binding protein with possible role in replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,010,382	0	A	G	100.0%	194.6 / NA	52	N140S (AAC→AGC)	uup	ATP‑binding protein with possible role in replication
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/27);  total (25/27)

ATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAAC  >  NC_000913/1010251‑1010518                                                                                                                                    |                                                                                                                                         atatCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGc                                                                                                                                  <  2:946609/140‑1 (MQ=255)       gAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGgcgc                                                                                                                            <  2:419554/140‑1 (MQ=255)       gAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGgcgc                                                                                                                            >  1:626100/1‑140 (MQ=255)        aaaCGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCa                                                                                                                           >  2:630930/1‑140 (MQ=255)          aCGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAAc                                                                                                                         <  2:431485/140‑1 (MQ=255)          aCGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAAc                                                                                                                         <  2:626100/140‑1 (MQ=255)          aCGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAAc                                                                                                                         >  1:617841/1‑140 (MQ=255)              tATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTggg                                                                                                                     >  2:257624/1‑140 (MQ=255)                tCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGt                                                                                                                   <  1:543150/140‑1 (MQ=255)                 cACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGtt                                                                                                                  <  2:558140/140‑1 (MQ=255)                  aCGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTa                                                                                                                 >  1:471063/1‑140 (MQ=255)                  aCGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTa                                                                                                                 <  1:637517/140‑1 (MQ=255)                  aCGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTa                                                                                                                 <  1:630930/140‑1 (MQ=255)                     atatTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGAt                                                                                                              >  1:35050/1‑140 (MQ=255)                           cgcgCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACt                                                                                                                        >  2:602284/1‑124 (MQ=255)                           cgcgCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACt                                                                                                                        <  1:602284/124‑1 (MQ=255)                           cgcgCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAAc                                                                                                        >  1:60037/1‑140 (MQ=255)                                 tGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTgcg                                                                                                  >  2:67692/1‑140 (MQ=255)                                       tgaACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTgtcg                                                                                            <  2:617841/140‑1 (MQ=255)                                          aCGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGtcgtcg                                                                                         <  1:686798/140‑1 (MQ=255)                                              cccGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCttt                                                                                     >  1:706577/1‑140 (MQ=255)                                              cccGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCttt                                                                                     >  1:473568/1‑140 (MQ=255)                                              cccGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCttt                                                                                     >  1:418421/1‑140 (MQ=255)                                                                  aCGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCggcggc                                                                             <  2:793231/128‑1 (MQ=255)                                                                  aCGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCggcggc                                                                             >  1:793231/1‑128 (MQ=255)                                                                  aCGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTaaa                                                                 <  2:60037/140‑1 (MQ=255)                                                                   cGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCGCAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAg                                                                <  2:168028/140‑1 (MQ=255)                                                                   cGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAg                                                                <  1:67692/140‑1 (MQ=255)                                                                   cGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAg                                                                <  1:845207/140‑1 (MQ=255)                                                                   cGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAg                                                                <  1:277473/140‑1 (MQ=255)                                                                         ggCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAg                                                                <  1:800011/134‑1 (MQ=255)                                                                         ggCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAg                                                                >  2:800011/1‑134 (MQ=255)                                                                                    aGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGAcgcgcg                                               <  1:257624/140‑1 (MQ=255)                                                                                    aGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGAcgcgcg                                               <  2:473568/140‑1 (MQ=255)                                                                                    aGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGAcgcgcg                                               <  2:471063/140‑1 (MQ=255)                                                                                          aGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAGGGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGAGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGTATTAGGACGCGCGCTGGTg                                         <  2:949398/140‑1 (MQ=255)                                                                                                    ccacAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACgcgcgc                                              >  1:831169/1‑125 (MQ=255)                                                                                                    ccacAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACgcgcgc                                              <  2:831169/125‑1 (MQ=255)                                                                                                    ccacAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCcgc                               >  2:254820/1‑140 (MQ=255)                                                                                                    ccacAACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCcgc                               >  2:231407/1‑140 (MQ=255)                                                                                                      acaACCTGTGGCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGTGGCATTAGGACGCGCGCTGGTGAGTAATCcgcgc                             >  2:917352/1‑140 (MQ=255)                                                                                                             gtgGCAGCTGGAAAACCGCATCCGCGAAGTCAGGGCGCAACTGTGGTTCGATCCTAACGTGGCGCTTTCGACGCTTTCCGGCGGCTGGGTGCGTAAAGCGGCATTCGGACGCGCGCTGGTGAGCAATCCGCGCGTGCTga                      >  2:288982/1‑139 (MQ=255)                                                                                                               ggCAGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTg                    >  1:734549/1‑140 (MQ=255)                                                                                                                  aGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCtt                 <  2:734549/140‑1 (MQ=255)                                                                                                                  aGCTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCtt                 >  2:879486/1‑140 (MQ=255)                                                                                                                    cTGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTtga               >  2:987482/1‑140 (MQ=255)                                                                                                                     tGGAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTtgat              >  1:255024/1‑140 (MQ=255)                                                                                                                      ggAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTtgatg             >  1:554027/1‑140 (MQ=255)                                                                                                                      ggAAAACCGCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTtgatg             <  2:554027/140‑1 (MQ=255)                                                                                                                              gCATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACa     >  1:638620/1‑140 (MQ=255)                                                                                                                               cATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACaa    >  1:485260/1‑140 (MQ=255)                                                                                                                               cATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACaa    <  1:523962/140‑1 (MQ=255)                                                                                                                               cATCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACaa    <  2:255024/140‑1 (MQ=255)                                                                                                                                 tCAGCGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAac  <  1:808486/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         ATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAAC  >  NC_000913/1010251‑1010518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑