Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,360,334	A→G	V192A (GTT→GCT)	puuA ←	glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,360,334	0	A	G	100.0%	219.4 / NA	59	V192A (GTT→GCT)	puuA	glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/30);  total (29/30)

TGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAACCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCA  >  NC_000913/1360199‑1360469                                                                                                                                        |                                                                                                                                        tGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTg                                                                                                                                     <  2:313802/140‑1 (MQ=255)  ggTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTgg                                                                                                                                    >  1:1053492/1‑140 (MQ=255)   gTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTGAGCCTGGc                                                                                                                                   >  1:879640/1‑140 (MQ=255)   gTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGc                                                                                                                                   <  2:731347/140‑1 (MQ=255)   gTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGc                                                                                                                                   >  2:488732/1‑140 (MQ=255)    tAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTg                                                                                                                                     <  2:904093/137‑1 (MQ=255)    tAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTg                                                                                                                                     >  1:904093/1‑137 (MQ=255)       aGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTtg                                                                                                                               >  1:50068/1‑140 (MQ=255)         gTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGcc                                                                                                                                       >  1:519587/1‑130 (MQ=255)         gTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTg                                                                                                                                     <  1:625091/132‑1 (MQ=255)         gTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTg                                                                                                                                     >  2:625091/1‑132 (MQ=255)             aTTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAATGGAGTAAGCCTGGCTTTGTGTAtt                                                                                                                         >  2:778566/1‑140 (MQ=255)             aTTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTGAGCCTGGCTTTGTGTAtt                                                                                                                         <  2:879640/140‑1 (MQ=255)                               gAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAgg                                                                                                       >  2:1069908/1‑140 (MQ=255)                                      cAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAg                                                                                                <  2:50068/140‑1 (MQ=255)                                        gCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAggg                                                                                              <  2:545646/140‑1 (MQ=255)                                        gCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAggg                                                                                              <  2:333690/140‑1 (MQ=255)                                          gACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCg                                                                                            <  2:713160/140‑1 (MQ=255)                                           aCCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCgg                                                                                           >  1:670456/1‑140 (MQ=255)                                            ccGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGt                                                                                          <  2:670456/140‑1 (MQ=255)                                            ccGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGt                                                                                          <  2:1053492/140‑1 (MQ=255)                                            ccGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGt                                                                                          <  2:866208/140‑1 (MQ=255)                                             cgcgCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCg                                                                                            <  2:917902/137‑1 (MQ=255)                                             cgcgCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCg                                                                                            >  1:917902/1‑137 (MQ=255)                                             cgcgCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGtt                                                                                         >  1:977926/1‑140 (MQ=255)                                                 ccATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAg                                                                                     >  1:259851/1‑140 (MQ=255)                                                    tCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATa                                                                                  <  1:251553/140‑1 (MQ=255)                                                              tCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCg                                                                        >  1:196529/1‑140 (MQ=255)                                                              tCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCg                                                                        <  2:196529/140‑1 (MQ=255)                                                                       aCTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTgg                                                               <  2:736390/140‑1 (MQ=255)                                                                         tGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGTTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCg                                                             <  2:678497/140‑1 (MQ=255)                                                                         tGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCg                                                             <  2:547147/140‑1 (MQ=255)                                                                           cgcCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGAt                                                           >  1:296058/1‑140 (MQ=255)                                                                           cgcCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGAt                                                           >  1:599256/1‑140 (MQ=255)                                                                            gcCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATc                                                          <  2:124653/140‑1 (MQ=255)                                                                            gcCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATc                                                          <  2:977926/140‑1 (MQ=255)                                                                              cAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCa                                                        <  1:1069908/140‑1 (MQ=255)                                                                                 ttCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTa                                                     <  1:488732/140‑1 (MQ=255)                                                                                      caATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAATGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAg                                                <  1:778566/140‑1 (MQ=255)                                                                                           tCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTc                                           >  1:853975/1‑140 (MQ=255)                                                                                           tCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTc                                           <  1:794930/140‑1 (MQ=255)                                                                                                  gCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGctct                                    <  2:599256/140‑1 (MQ=255)                                                                                                   cACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTa                                   >  1:237992/1‑140 (MQ=255)                                                                                                   cACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGGAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTa                                   >  2:976593/1‑140 (MQ=255)                                                                                                      gTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCg                                >  2:593512/1‑140 (MQ=255)                                                                                                      gTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCg                                >  2:212503/1‑140 (MQ=255)                                                                                                      gTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCg                                >  1:614505/1‑140 (MQ=255)                                                                                                       tCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAg                                        <  2:29240/131‑1 (MQ=255)                                                                                                       tCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAg                                        >  1:29240/1‑131 (MQ=255)                                                                                                                            tCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCcgcg          >  1:745963/1‑140 (MQ=255)                                                                                                                            tCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCcgcg          >  2:322953/1‑140 (MQ=255)                                                                                                                            tCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCcgcg          >  1:793148/1‑140 (MQ=255)                                                                                                                            tCAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCcgcg          <  2:160646/140‑1 (MQ=255)                                                                                                                             cAACGGAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCgcgc         <  1:322953/140‑1 (MQ=255)                                                                                                                                  gAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGgcg    <  2:793148/140‑1 (MQ=255)                                                                                                                                  gAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGgcg    >  1:77481/1‑140 (MQ=255)                                                                                                                                  gAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGgcg    >  1:118591/1‑140 (MQ=255)                                                                                                                                  gAGTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGgcg    >  2:210057/1‑140 (MQ=255)                                                                                                                                    gTAAGCCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCa  <  2:259851/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCATCAATATCATTGAGCACGTCGGCGAAGTGGTTGAGGTTATCAACGGAGTAAACCTGGCTTTGTGTATTGCGGTCATCGGTGCCAGGCGCGCAGGGCGGTTGCAGATACCCTTCAGCGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCA  >  NC_000913/1360199‑1360469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑