Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,376,381	T→C	R116R (CGT→CGC)	ycjR →	putative ketohexose‑3‑epimerase YcjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,376,381	0	T	C	100.0%	185.5 / NA	50	R116R (CGT→CGC)	ycjR	putative ketohexose‑3‑epimerase YcjR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/23);  total (27/23)

CGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAC  >  NC_000913/1376250‑1376506                                                                                                                                     |                                                                                                                              cgtcgtCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGa                                                                                                                       >  1:476696/1‑140 (MQ=255)       cTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGa                                                                                                                 <  1:601104/140‑1 (MQ=255)                ttAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAAt                                                                                                        >  2:878554/1‑140 (MQ=255)                ttAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAAt                                                                                                        >  1:979035/1‑140 (MQ=255)                        gATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGgtgagtg                                                                                                >  1:163267/1‑140 (MQ=255)                             aGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTcc                                                                                           >  1:169356/1‑140 (MQ=255)                             aGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTcc                                                                                           >  2:680934/1‑140 (MQ=255)                              gcgcATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTccc                                                                                          >  1:379129/1‑140 (MQ=255)                                  tttgtggAAGCGCGGGCAG‑AGGGGGGGGGAAAAGCATCGGTCTTCCGGGCTGGGTGGGGCATGTTAACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTgcg                                                                                      <  2:286147/134‑1 (MQ=255)                                        gAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACt                                                                                >  1:994869/1‑140 (MQ=255)                                        gAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACt                                                                                >  1:688128/1‑140 (MQ=255)                                              cTGGCAGAAGTGGGGGGTAAGGGCACC‑GGCGTGCCGGCGGCGGGGGGCTTTTTTACCTCCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACa                                                                          <  2:613425/140‑1 (MQ=255)                                               tGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAg                                                                         >  1:261833/1‑140 (MQ=255)                                                ggCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAgg                                                                        >  1:466544/1‑140 (MQ=255)                                                ggCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAgg                                                                        >  1:969779/1‑140 (MQ=255)                                                           gCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGt                                                             >  2:1034154/1‑140 (MQ=255)                                                           gCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGt                                                             >  2:342592/1‑140 (MQ=255)                                                             ggTAAGGGCATC‑GTCGTTCCGGCGGGGGGGGGCTTGTTTCCCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTAc                                                           <  2:379129/140‑1 (MQ=255)                                                               tAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACATGTCGCCGCGCGTACCg                                                         >  1:967287/1‑140 (MQ=255)                                                                 aaGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGa                                                       >  2:229788/1‑140 (MQ=255)                                                                  aGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGaa                                                      <  1:878554/140‑1 (MQ=255)                                                                    gCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAAcc                                                    <  2:994869/140‑1 (MQ=255)                                                                    gCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAAcc                                                    <  2:688128/140‑1 (MQ=255)                                                                          gtcgtTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCgtggt                                               >  1:742159/1‑140 (MQ=255)                                                                          gtcgtTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCgtggt                                               >  2:214711/1‑140 (MQ=255)                                                                              ttCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTAt                                           >  1:1075705/1‑140 (MQ=255)                                                                                   gCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGa                                      >  2:172131/1‑140 (MQ=255)                                                                                            ggCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTaaa                             <  2:979035/140‑1 (MQ=255)                                                                                               aTGTTTCCCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCg                          <  2:169356/140‑1 (MQ=255)                                                                                                 gTTTACCTTCCGCTTACCGCTGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCt                        >  1:758949/1‑140 (MQ=255)                                                                                                 gTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCt                        >  1:307279/1‑140 (MQ=255)                                                                                                  tttACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTa                       <  1:229788/140‑1 (MQ=255)                                                                                                   ttACCTTCCGCTTACCGCTGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTAt                      <  2:758949/140‑1 (MQ=255)                                                                                                   ttACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTAt                      <  2:307279/140‑1 (MQ=255)                                                                                                     cccTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGTGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCa                    <  2:986374/139‑1 (MQ=255)                                                                                                      ccTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAg                   <  1:342592/140‑1 (MQ=255)                                                                                                       cTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAgg                  <  2:261833/140‑1 (MQ=255)                                                                                                       cTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAgg                  <  1:340872/140‑1 (MQ=255)                                                                                                       cTTCCGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAgg                  <  2:969779/140‑1 (MQ=255)                                                                                                       cTTCCGCTACACGCTGATGGCCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAgg                  <  2:679840/140‑1 (MQ=255)                                                                                                          ccGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATc               >  2:166159/1‑140 (MQ=255)                                                                                                          ccGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATc               >  1:67473/1‑140 (MQ=255)                                                                                                           cGCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCa              >  1:857988/1‑140 (MQ=255)                                                                                                            gCTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCat             <  2:466544/140‑1 (MQ=255)                                                                                                             cTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCata            <  2:1075705/140‑1 (MQ=255)                                                                                                             cTTACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCata            <  2:476696/140‑1 (MQ=255)                                                                                                              ttACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCatat           <  1:171598/140‑1 (MQ=255)                                                                                                               tACCGCCGATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATg          <  2:967287/140‑1 (MQ=255)                                                                                                                   gccgATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCa      <  1:214711/140‑1 (MQ=255)                                                                                                                   gccgATGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCa      >  2:425750/1‑140 (MQ=255)                                                                                                                       aTGACCTCGCCGCGCAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAacac  >  1:217228/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                              CGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATC‑GTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAC  >  NC_000913/1376250‑1376506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑