Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,512,214	C→T	G187G (GGC→GGT)	ydcS →	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,512,214	0	C	T	100.0%	86.9 / NA	26	G187G (GGC→GGT)	ydcS	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/11);  total (15/11)

CAATGGGGGCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGA  >  NC_000913/1512080‑1512347                                                                                                                                       |                                                                                                                                      cAATGGGGGCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCctat                                                                                                                                  <  1:1057656/140‑1 (MQ=255)    tGGGGGCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCctatcta                                                                                                                               >  2:842278/1‑140 (MQ=255)      ggggCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCtata                                                                                                                             >  2:802754/1‑140 (MQ=255)                    tgATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCg                                                                                                               <  2:164680/140‑1 (MQ=255)                                            cGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCg                                                                                       <  2:625257/140‑1 (MQ=255)                                                gccgccGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGt                                                                                   >  1:756222/1‑140 (MQ=255)                                                             tGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGGTGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGAAGTTGGGCATCAGCGa                                                                      <  2:787315/140‑1 (MQ=255)                                                             tGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGa                                                                      <  1:751793/140‑1 (MQ=255)                                                               gCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATc                                                                    <  1:842278/140‑1 (MQ=255)                                                                         tttGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACt                                                          >  2:314617/1‑140 (MQ=255)                                                                             ttgAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCAcc                                                      >  1:1045732/1‑140 (MQ=255)                                                                              tgAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCg                                                     <  2:756222/140‑1 (MQ=255)                                                                                              gACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGc                                     <  1:533596/140‑1 (MQ=255)                                                                                                   cAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCATTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGc                                >  2:834439/1‑140 (MQ=255)                                                                                                   cAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGc                                >  2:721301/1‑140 (MQ=255)                                                                                                           aTAAAGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGtg                        >  2:144872/1‑140 (MQ=255)                                                                                                             aaaGGCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGtgct                      <  1:802754/140‑1 (MQ=255)                                                                                                                 gCCGCGTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTgcgc                  >  2:360363/1‑140 (MQ=255)                                                                                                                   cgcgTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTgcgcgc                >  1:67213/1‑140 (MQ=255)                                                                                                                   cgcgTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTgcgcgc                >  1:913028/1‑140 (MQ=255)                                                                                                                     cgTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTc              >  2:701112/1‑140 (MQ=255)                                                                                                                     cgTTCAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTc              >  1:443524/1‑140 (MQ=255)                                                                                                                         cAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAaca          <  2:209109/140‑1 (MQ=255)                                                                                                                         cAGGCTTATGATGGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAaca          <  1:762494/140‑1 (MQ=255)                                                                                                                                 tgatgGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGa  >  2:607650/1‑140 (MQ=255)                                                                                                                                 tgatgGTCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGa  >  2:978901/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      CAATGGGGGCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGA  >  NC_000913/1512080‑1512347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑