Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,514,354	T→C	V175A (GTG→GCG)	ydcU →	putative ABC transporter membrane subunit YdcU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,514,354	0	T	C	100.0%	107.7 / NA	30	V175A (GTG→GCG)	ydcU	putative ABC transporter membrane subunit YdcU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

GCCGATGTGGGCGAGCTACATTGTTAAAGCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCT  >  NC_000913/1514220‑1514487                                                                                                                                       |                                                                                                                                      gCCGATGTGGGCGAGCTACATTGTTAAAGCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCggcgggcg                                                                                                                                  >  2:184993/1‑140 (MQ=255) gCCGATGTGGGCGAGCTACATTGTTAAAGCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCggggg                                                                                                                                  >  1:297731/1‑138 (MQ=255)      tgtgGGCGAGCTACATTGTTAAAGCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAAt                                                                                                                             >  2:79835/1‑140 (MQ=255)         gggCGAGCTACATTGTTAAAGCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACg                                                                                                                          <  1:393030/140‑1 (MQ=255)                cTACATTGTTAAAGCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCaa                                                                                                                   >  2:1066318/1‑140 (MQ=255)                         tAAAGCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGc                                                                                                          >  2:481195/1‑140 (MQ=255)                             gCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGGGGAAATACGCTTGCAACTTCCCGGCTcgg                                                                                                      >  2:959846/1‑140 (MQ=255)                             gCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTggg                                                                                                      >  1:507433/1‑140 (MQ=255)                                tATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGGAATACGCCGTTAACTTTCCGGCCCGGgcg                                                                                                   >  1:138015/1‑140 (MQ=255)                                tATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGgcg                                                                                                   <  1:481195/140‑1 (MQ=255)                                        gACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTgg                                                                                           <  1:145623/140‑1 (MQ=255)                                          cGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGGAATACGCGGGCTACTTCCGGGCTGGGGCGCTTTCTgggg                                                                                         >  2:772111/1‑138 (MQ=255)                                          cGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGgtg                                                                                         <  2:297731/140‑1 (MQ=255)                                           gTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGgtgt                                                                                        <  2:507433/140‑1 (MQ=255)                                            ttATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGGAATACGCCGTCAACTTTCGGGCTGGGGCGCTTTTTGGGGtt                                                                                       >  2:539429/1‑140 (MQ=255)                                            ttATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGtt                                                                                       <  1:866737/140‑1 (MQ=255)                                             tATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGttt                                                                                      >  2:163124/1‑140 (MQ=255)                                              aTTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGgc                                                                                                    >  1:18789/1‑125 (MQ=255)                                              aTTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGgc                                                                                                    <  2:18789/125‑1 (MQ=255)                                               ttGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCTGGCTGGGGCGCTTTCTGGTGTTtct                                                                                    <  2:496014/140‑1 (MQ=255)                                               ttGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTtct                                                                                    <  1:184993/140‑1 (MQ=255)                                                 gCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTctct                                                                                  <  1:1066318/140‑1 (MQ=255)                                                            aTGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTg                                                                       <  1:79835/140‑1 (MQ=255)                                                            aTGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTg                                                                       <  1:539429/140‑1 (MQ=255)                                                                       cAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGAt                                                            <  1:959846/140‑1 (MQ=255)                                                                                     acaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAgg                                              <  1:163124/140‑1 (MQ=255)                                                                                                  gAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCg                                 >  1:344112/1‑140 (MQ=255)                                                                                                        ctgctgACTGCGTTCCTTACATTACCTGCGGCGGGCGGAAAAACGCTGTCAACTTCCGGGCTGGGGGGCTTTCTGGGGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTgcc                           >  2:740538/1‑140 (MQ=255)                                                                                                                 gCGTTCCTTACATTACCTGCGGCGGGCGGGAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAtt                  >  2:881647/1‑140 (MQ=255)                                                                                                                     agacagcATTACCTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCGTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgttg              <  1:747101/134‑1 (MQ=255)                                                                                                                                 cTGCGGCGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGGGCTTTCTGGTGTTTCTCTATATCTTGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCt  >  2:813378/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      GCCGATGTGGGCGAGCTACATTGTTAAAGCCTATGCCTGGACGTTATTGCTGGCAAAAGATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCT  >  NC_000913/1514220‑1514487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑