Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,543,373 A→G S230P (TCA→CCA)  narU ← nitrate/nitrite transporter NarU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,543,3730AG100.0% 191.0 / NA 51S230P (TCA→CCA) narUnitrate/nitrite transporter NarU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/25);  total (26/25)

CCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAG  >  NC_000913/1543240‑1543508
                                                                                                                                     |                                                                                                                                       
cccGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATgc                                                                                                                                   >  2:212422/1‑140 (MQ=255)
 ccGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATgcg                                                                                                                                  <  2:783968/140‑1 (MQ=255)
           aCCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTgg                                                                                                                        <  1:212422/140‑1 (MQ=255)
                tAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATc                                                                                                                   >  1:426316/1‑140 (MQ=255)
                                 gCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCa                                                                                                  <  2:11846/140‑1 (MQ=255)
                                  cAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCaa                                                                                                 >  2:517492/1‑140 (MQ=255)
                                   aaGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCaaa                                                                                                <  1:517492/140‑1 (MQ=255)
                                         aaGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATg                                                                                          <  1:979150/140‑1 (MQ=255)
                                              ggCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTATCCAAATCCATGCGGCa                                                                                     >  2:142617/1‑140 (MQ=255)
                                               gCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCAt                                                                                    <  1:911508/140‑1 (MQ=255)
                                                 tCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTc                                                                                  >  1:28589/1‑140 (MQ=255)
                                                 tCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTc                                                                                  >  2:580382/1‑140 (MQ=255)
                                                 tCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTc                                                                                  >  1:663190/1‑140 (MQ=255)
                                                       gcCAGAGATGCAGGCGTTGTAATACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGc                                                                            <  1:780438/140‑1 (MQ=255)
                                                           gagaTGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACa                                                                        <  1:580382/140‑1 (MQ=255)
                                                            agaTGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACAt                                                                       >  1:896906/1‑140 (MQ=255)
                                                                      gTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCg                                                             >  1:228960/1‑140 (MQ=255)
                                                                       ttGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGt                                                            >  2:547004/1‑140 (MQ=255)
                                                                       ttGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGt                                                            <  1:547004/140‑1 (MQ=255)
                                                                          tAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCgg                                                         >  1:549525/1‑140 (MQ=255)
                                                                           aaGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGc                                                        <  2:228960/140‑1 (MQ=255)
                                                                              aCAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTg                                                     >  2:295902/1‑140 (MQ=255)
                                                                                 gggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGcc                                                       <  1:209839/135‑1 (MQ=255)
                                                                                 gggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGcc                                                       >  2:209839/1‑135 (MQ=255)
                                                                                  ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGTCAGCGACATCACCGAACCGTCGGCCTGCGGt                                                 >  2:701557/1‑140 (MQ=255)
                                                                                  ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGCCATCACCGAACCGTCGGCCTGCgga                                                 >  1:994022/1‑139 (MQ=255)
                                                                                  ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGt                                                 >  2:711811/1‑140 (MQ=255)
                                                                                  ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGt                                                 >  1:112042/1‑140 (MQ=255)
                                                                                  ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGt                                                 >  1:688457/1‑140 (MQ=255)
                                                                                     gCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACg                                              <  2:720474/140‑1 (MQ=255)
                                                                                         gTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCAt                                          <  2:426316/140‑1 (MQ=255)
                                                                                          tCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCAtt                                         <  2:663190/140‑1 (MQ=255)
                                                                                            ggCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGa                                       >  1:693630/1‑140 (MQ=255)
                                                                                                 ttGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCg                                  <  2:896906/140‑1 (MQ=255)
                                                                                                 ttGAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCg                                  <  2:688457/140‑1 (MQ=255)
                                                                                                   gAGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCgag                                >  2:1078930/1‑140 (MQ=255)
                                                                                                    aGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGTCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                               <  1:701557/140‑1 (MQ=255)
                                                                                                    aGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                               <  1:957274/140‑1 (MQ=255)
                                                                                                    aGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                               <  2:135826/140‑1 (MQ=255)
                                                                                                    aGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                               <  1:245844/140‑1 (MQ=255)
                                                                                                    aGGCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                               <  2:112042/140‑1 (MQ=255)
                                                                                                     ggCGCGTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGaa                              <  2:784590/140‑1 (MQ=255)
                                                                                                         cgTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGc                          >  1:917227/1‑140 (MQ=255)
                                                                                                          gTGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCa                         >  1:937507/1‑140 (MQ=255)
                                                                                                           tGAACTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCaa                        >  1:625822/1‑140 (MQ=255)
                                                                                                               cTGGCGATATCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATAc                    <  1:1078930/140‑1 (MQ=255)
                                                                                                                       atCATTCATCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACaa            >  1:122845/1‑140 (MQ=255)
                                                                                                                        tcattcatCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACaaa           >  2:358533/1‑140 (MQ=255)
                                                                                                                          attcatCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACaaaaa         >  2:749960/1‑140 (MQ=255)
                                                                                                                              atCCCTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGAc     <  2:1057937/140‑1 (MQ=255)
                                                                                                                                 ccTGGCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAg  <  1:749960/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
CCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAG  >  NC_000913/1543240‑1543508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑