Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,748,407	T→C	V426A (GTC→GCC)	ydhS →	FAD/NAD(P) binding domain‑containing protein YdhS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,748,407	0	T	C	100.0%	171.7 / NA	46	V426A (GTC→GCC)	ydhS	FAD/NAD(P) binding domain‑containing protein YdhS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/25);  total (21/25)

GGTTTTCATCGATAATTATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCC  >  NC_000913/1748273‑1748539                                                                                                                                       |                                                                                                                                     ggTTTTCATCGATAATTATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGa                                                                                                                                 >  2:184446/1‑140 (MQ=255)    tttCATCGATAATTATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGaaaa                                                                                                                              <  1:129095/140‑1 (MQ=255)       cATCGATAATTATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACgg                                                                                                                           <  2:630478/140‑1 (MQ=255)       cATCGATAATTATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACgg                                                                                                                           <  1:76420/140‑1 (MQ=255)            aTAATTATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGac                                                                                                                      <  2:243302/140‑1 (MQ=255)            aTAATTATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGac                                                                                                                      <  1:639446/140‑1 (MQ=255)             tAATTATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGaca                                                                                                                     <  1:291270/140‑1 (MQ=255)                 tATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAg                                                                                                                 <  1:183972/140‑1 (MQ=255)                   tGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCt                                                                                                               <  2:193236/140‑1 (MQ=255)                   tGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCt                                                                                                               >  2:263928/1‑140 (MQ=255)                       gCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTc                                                                                                           >  2:578802/1‑140 (MQ=255)                          aTCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGtt                                                                                                        <  2:449443/140‑1 (MQ=255)                           tCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGttt                                                                                                       >  1:727154/1‑140 (MQ=255)                             ccTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGa                                                                                                     <  1:1018621/140‑1 (MQ=255)                             ccTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGa                                                                                                     <  1:848896/140‑1 (MQ=255)                                tCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGt                                                                                                  <  1:578802/140‑1 (MQ=255)                                         aTTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGa                                                                                                     >  1:133915/1‑128 (MQ=255)                                         aTTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGa                                                                                                     <  2:133915/128‑1 (MQ=255)                                         aTTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGa                                                                                         <  2:46818/140‑1 (MQ=255)                                          ttCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGAt                                                                                        >  1:276486/1‑140 (MQ=255)                                               cgCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCcg                                                                                   <  1:393123/140‑1 (MQ=255)                                                gCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCcgc                                                                                  >  2:138940/1‑140 (MQ=255)                                                        gCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACg                                                                          <  1:966755/140‑1 (MQ=255)                                                              cGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCt                                                                    >  1:563204/1‑140 (MQ=255)                                                               gTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCtt                                                                   <  1:138940/140‑1 (MQ=255)                                                                  aaGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTaaa                                                                >  1:988477/1‑140 (MQ=255)                                                                     cGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTg                                                             <  2:727154/140‑1 (MQ=255)                                                                                catATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATccc                                                  <  1:184446/140‑1 (MQ=255)                                                                                       tcGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCt                                           >  2:132469/1‑140 (MQ=255)                                                                                         gCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATccc                                                  >  2:734180/1‑131 (MQ=255)                                                                                         gCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATccc                                                  <  1:734180/131‑1 (MQ=255)                                                                                         gCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTcc                                             <  1:627035/136‑1 (MQ=255)                                                                                         gCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTcc                                             >  2:627035/1‑136 (MQ=255)                                                                                         gCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTgg                                         >  2:247850/1‑140 (MQ=255)                                                                                         gCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTgg                                         >  1:470922/1‑140 (MQ=255)                                                                                         gCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTgg                                         >  1:233393/1‑140 (MQ=255)                                                                                               ggTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATccc                                                  >  2:942017/1‑125 (MQ=255)                                                                                               ggTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATccc                                                  <  1:942017/125‑1 (MQ=255)                                                                                               ggTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAcg                                   >  1:180982/1‑140 (MQ=255)                                                                                                      aCTACAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCaa                            <  2:988477/140‑1 (MQ=255)                                                                                                         aCAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTa                         >  1:1055246/1‑140 (MQ=255)                                                                                                          cAAAATGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTAc                        >  2:955630/1‑140 (MQ=255)                                                                                                              aTGGAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATAACAGaa                    <  2:563204/140‑1 (MQ=255)                                                                                                                ggAAATTAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGaaaa                  >  1:1030725/1‑140 (MQ=255)                                                                                                                     ttAATGAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAggg             <  2:1030725/140‑1 (MQ=255)                                                                                                                          gAGTCGCGCACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGa        <  2:233393/140‑1 (MQ=255)                                                                                                                                cgcACCGCCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATccc  >  1:949924/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     GGTTTTCATCGATAATTATGCGGCAATCCCTTCAGAGTCTATTCGTCGCCTACTTGCCTTACGTGAAGCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCC  >  NC_000913/1748273‑1748539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑