Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,763,967 T→C T15A (ACC→GCC)  sufB ← Fe‑S cluster scaffold complex subunit SufB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,763,9670TC100.0% 175.3 / NA 47T15A (ACC→GCC) sufBFe‑S cluster scaffold complex subunit SufB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/23);  total (24/23)

TCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCAC  >  NC_000913/1763837‑1764100
                                                                                                                                  |                                                                                                                                     
tCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGtttt                                                                                                                              <  2:177629/140‑1 (MQ=255)
 ccAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTg                                                                                                                             <  1:785542/140‑1 (MQ=255)
            tCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGt                                                                                                                  >  2:834042/1‑140 (MQ=255)
            tCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGt                                                                                                                  <  1:241715/140‑1 (MQ=255)
            tCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGt                                                                                                                  <  1:248894/140‑1 (MQ=255)
              cGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTACCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTg                                                                                                                >  1:708527/1‑140 (MQ=255)
                 cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCtt                                                                                                             <  1:283029/140‑1 (MQ=255)
                 cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCtt                                                                                                             <  1:846999/140‑1 (MQ=255)
                  tCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTc                                                                                                            <  1:431699/140‑1 (MQ=255)
                    aTTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAg                                                                                                          <  2:296526/140‑1 (MQ=255)
                    aTTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAg                                                                                                          <  1:499895/140‑1 (MQ=255)
                      tACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGATTTTGACATCGTCAGTTGCTTCAGTa                                                                                                        >  2:688731/1‑140 (MQ=255)
                         gCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGATTTTGACATCGTCAGTTGCTTCAGTATTa                                                                                                     <  1:688731/140‑1 (MQ=255)
                           ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCGGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACg                                                                                                   <  1:685597/140‑1 (MQ=255)
                           ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACg                                                                                                   <  1:447927/140‑1 (MQ=255)
                           ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACg                                                                                                   <  1:197417/140‑1 (MQ=255)
                           ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACg                                                                                                   <  1:1028585/140‑1 (MQ=255)
                              gCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACgaga                                                                                                >  2:410933/1‑140 (MQ=255)
                              gCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACgaga                                                                                                >  1:737589/1‑140 (MQ=255)
                              gCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAACAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACg                                                                                                   <  2:737278/137‑1 (MQ=255)
                              gCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAACAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACg                                                                                                   >  1:737278/1‑137 (MQ=255)
                                               caccTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGACACATAGTACCGCCTATAc                                                                               >  1:434418/1‑140 (MQ=255)
                                                       cGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGc                                                                                     >  2:404799/1‑126 (MQ=255)
                                                       cGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGc                                                                                     <  1:404799/126‑1 (MQ=255)
                                                       cGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGc                                                                       >  1:961561/1‑140 (MQ=255)
                                                             acagcTTTGCCAGCTCATCGGGGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCATTTTTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCgc                                                                 <  1:654571/136‑1 (MQ=255)
                                                                   ttcccAGCTCATCGGTGGCTACTGGGGTAAAGAATCTTTTTTTATATTCCAGCGGCCCGCCGCCCCGGTTTTTAACACCGTCATTTTCTTAATTATTCCGAAAAATAGTACCGCCTATACCCCAAAGCTTTCgccacagc                                                           <  2:811467/137‑1 (MQ=255)
                                                                   ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCgccacagc                                                           <  2:961561/140‑1 (MQ=255)
                                                                   ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCgccacagc                                                           >  2:126298/1‑140 (MQ=255)
                                                                    tGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCgccacagcc                                                          >  1:224289/1‑140 (MQ=255)
                                                                    tGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCgccacagcc                                                          >  1:339808/1‑140 (MQ=255)
                                                                    tGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCgcca                                                               >  2:326091/1‑135 (MQ=255)
                                                                    tGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCgcca                                                               <  1:326091/135‑1 (MQ=255)
                                                                    tGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCcccacagcc                                                          >  2:630986/1‑140 (MQ=255)
                                                                        aGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGACACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCAcat                                                      <  2:434418/140‑1 (MQ=255)
                                                                          cTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCAcattc                                                    >  2:673426/1‑140 (MQ=255)
                                                                            cATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTCCCCCACAGCCAcatctgt                                                  >  2:654571/1‑136 (MQ=255)
                                                                               gggTGGCTAACTGGGTGAGGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                               <  1:692350/139‑1 (MQ=255)
                                                                               gggTGGCTAACTGGGTAAAGAATCTTTCTTTATAATTCAGCGGGCCGCCGGCCCGGGTTTTGACATCGTCAGTTGCTTCATTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                               <  2:224289/139‑1 (MQ=255)
                                                                               gggTGGCAAACTGGGTAAAAAATCTTTCTTTATAACTCAGCGGCCCGCCGCCCCAGGTTTTGACACCGTCAGTTGCTCCAGTATTACGAGACATAGTCCCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                               <  2:759930/139‑1 (MQ=255)
                                                                               cGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCGGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                               <  1:834042/140‑1 (MQ=255)
                                                                                ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                              >  1:810853/1‑140 (MQ=255)
                                                                                     ctaccggggTGAAGAATCCTTTTTTATAATTCAGCGGGCCGCCGGCCCAGTTTTTGACATCGTAAGTTGTTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCtt                                         <  2:1034694/134‑1 (MQ=255)
                                                                                            ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGGCATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGtt                                  >  1:543068/1‑140 (MQ=255)
                                                                                            ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGtt                                  >  2:964728/1‑140 (MQ=255)
                                                                                            ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGtt                                  >  1:744668/1‑140 (MQ=255)
                                                                                                   aaTCCTTCTTTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAAt                           <  1:673426/140‑1 (MQ=255)
                                                                                                       cttcttTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                       >  2:1076747/1‑140 (MQ=255)
                                                                                                        ttttttATAATCCGGGGGGCCGCCGCCCCGGGTTTTGACATCTTCAGTTGTTTCATTTTTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGaa                      <  2:810853/137‑1 (MQ=255)
                                                                                                        ttcttTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGaa                      >  2:84565/1‑140 (MQ=255)
                                                                                                          cttTATAATTCAGCGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAata                    <  2:708527/140‑1 (MQ=255)
                                                                                                                       cGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc       >  2:724317/1‑140 (MQ=255)
                                                                                                                       cGGGCCGCCGGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc       >  1:539979/1‑140 (MQ=255)
                                                                                                                            cgccgGCCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCac  >  1:123810/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                     
TCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCAC  >  NC_000913/1763837‑1764100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑