Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,165,953	T→C	S96P (TCC→CCC)	yegQ →	putative peptidase YegQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,953	0	T	C	100.0%	131.1 / NA	36	S96P (TCC→CCC)	yegQ	putative peptidase YegQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/20);  total (16/20)

GGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGTCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCAAATGGGCCTGACCCGCGTGAT  >  NC_000913/2165818‑2166083                                                                                                                                        |                                                                                                                                   ggCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGa                                                                                                                                >  1:502888/1‑140 (MQ=255)        aTGAAGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAggg                                                                                                                         <  2:499135/140‑1 (MQ=255)            aGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGa                                                                                                                     <  2:502888/140‑1 (MQ=255)             gCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGAt                                                                                                                    >  1:928197/1‑140 (MQ=255)             gCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCAGATCCAGGGCTGAt                                                                                                                    >  1:922245/1‑140 (MQ=255)                 aCGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATg                                                                                                                >  1:64131/1‑140 (MQ=255)                       tGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTg                                                                                                          >  1:614936/1‑140 (MQ=255)                         gggAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCg                                                                                                        >  2:93746/1‑140 (MQ=255)                          ggAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGt                                                                                                       <  1:954921/140‑1 (MQ=255)                             aaaaGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAg                                                                                                    <  2:713248/140‑1 (MQ=255)                             aaaaGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAg                                                                                                    <  2:541566/140‑1 (MQ=255)                             aaaaGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAg                                                                                                    >  2:39303/1‑140 (MQ=255)                             aaaaGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAg                                                                                                    <  1:110707/140‑1 (MQ=255)                               aaGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCa                                                                                                  <  2:348866/140‑1 (MQ=255)                               aaGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCa                                                                                                  <  1:261057/140‑1 (MQ=255)                                                    aTTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCa                                                                             <  2:64131/140‑1 (MQ=255)                                                     ttGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCAc                                                                            >  1:947585/1‑140 (MQ=255)                                                        cACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCtt                                                                         >  2:692679/1‑140 (MQ=255)                                                           cGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCg                                                                      >  2:4325/1‑140 (MQ=255)                                                              acaACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTg                                                                   <  2:928197/140‑1 (MQ=255)                                                              acaACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCAGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTg                                                                   <  2:922245/140‑1 (MQ=255)                                                                  cGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAgg                                                               >  1:245707/1‑140 (MQ=255)                                                                          tGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGcc                                                       >  2:406653/1‑140 (MQ=255)                                                                                   ttATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTgg                                              >  2:504475/1‑140 (MQ=255)                                                                                                     cGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTgg                            <  1:4325/140‑1 (MQ=255)                                                                                                     cGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTgg                            <  2:828298/140‑1 (MQ=255)                                                                                                      ggtggtGGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGgc                           <  1:93746/140‑1 (MQ=255)                                                                                                       gtggtgGAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGgca                          >  1:526397/1‑140 (MQ=255)                                                                                                            ggAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCaaa                     <  1:39303/140‑1 (MQ=255)                                                                                                            ggAAATGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCaaa                     <  2:1092482/140‑1 (MQ=255)                                                                                                               aaTGGGGCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCAAATgg                  >  2:829004/1‑140 (MQ=255)                                                                                                                     gCCGGATGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCAAATGGGCCTGa            >  1:504881/1‑140 (MQ=255)                                                                                                                          aTGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCAAATGGGCCTGACCcgc       <  1:829004/140‑1 (MQ=255)                                                                                                                           tGCGCTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCAAATGGGCCTGACCcgcg      <  2:442950/140‑1 (MQ=255)                                                                                                                               cTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCAAATGGGCCTGACCCGCGTGAt  <  2:526397/140‑1 (MQ=255)                                                                                                                               cTGATTATGCCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCAAATGGGCCTGACCCGCGTGAt  <  1:692679/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   GGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGCCGGATGCGCTGATTATGTCCGATCCAGGGCTGATTATGCTGGTGCGTGAGCACTTCCCTGAAATGCCGATCCACCTTTCGGTGCAGGCTAACGCCGTGAACTGGGCGACGGTGAAATTCTGGCAGCAAATGGGCCTGACCCGCGTGAT  >  NC_000913/2165818‑2166083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑