Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	241,304	A→G	V667A (GTG→GCG)	fadE ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	241,304	0	A	G	100.0%	235.6 / NA	64	V667A (GTG→GCG)	fadE	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/35);  total (29/35)

TGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCA  >  NC_000913/241169‑241438                                                                                                                                        |                                                                                                                                       tGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACcgcgcg                                                                                                                                    >  2:958576/1‑140 (MQ=255)   aCCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGt                                                                                                                                  >  2:425354/1‑140 (MQ=255)   aCCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGt                                                                                                                                  >  1:913195/1‑140 (MQ=255)    ccGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGtt                                                                                                                                 >  2:427322/1‑140 (MQ=255)       cgACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCgg                                                                                                                              >  2:317042/1‑140 (MQ=255)        gACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCggg                                                                                                                             <  1:1096027/140‑1 (MQ=255)        gACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCggg                                                                                                                             <  1:165485/140‑1 (MQ=255)        gACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCggg                                                                                                                             <  1:940501/140‑1 (MQ=255)          ccAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGaa                                                                                                                           >  1:351018/1‑140 (MQ=255)              tGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGttt                                                                                                                       >  2:55304/1‑140 (MQ=255)              tGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGttt                                                                                                                       >  1:759452/1‑140 (MQ=255)                  ggAACGGGTGTCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCa                                                                                                                   <  2:679142/140‑1 (MQ=255)                  ggAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCCCCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCa                                                                                                                   <  2:759452/140‑1 (MQ=255)                    aaCGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGt                                                                                                                 <  2:299200/140‑1 (MQ=255)                        ggTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAat                                                                                                             <  1:1043293/140‑1 (MQ=255)                        ggTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAat                                                                                                             >  1:550180/1‑140 (MQ=255)                         gTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAatc                                                                                                            >  2:39427/1‑140 (MQ=255)                          tGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAatca                                                                                                           >  1:608760/1‑140 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGTAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  1:765971/140‑1 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  2:259099/140‑1 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  2:138189/140‑1 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  2:1030732/140‑1 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  1:96464/140‑1 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  1:39427/140‑1 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  1:427322/140‑1 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  2:370476/140‑1 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  2:608760/140‑1 (MQ=255)                                       ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTATTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  1:666308/140‑1 (MQ=255)                                         gTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              >  2:501502/1‑138 (MQ=255)                                         gTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                              <  1:501502/138‑1 (MQ=255)                                            aaaTCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGcc                                                                                         >  2:1093749/1‑140 (MQ=255)                                                cTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGAt                                                                                     <  2:351018/140‑1 (MQ=255)                                                  tCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATAc                                                                                   <  1:317042/140‑1 (MQ=255)                                                  tCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATAc                                                                                   <  2:913195/140‑1 (MQ=255)                                                     ccACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGTATCCGCGCGTTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAgc                                                                                <  2:290835/140‑1 (MQ=255)                                                     ccACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAgc                                                                                <  2:550180/140‑1 (MQ=255)                                                     ccACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAgc                                                                                <  1:102270/140‑1 (MQ=255)                                                           tATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCt                                                                          >  2:646684/1‑140 (MQ=255)                                                               aTCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTa                                                                      <  1:425354/140‑1 (MQ=255)                                                                   aGCTTGTCAGCAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGcc                                                                  >  2:680486/1‑140 (MQ=255)                                                                   aGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGcc                                                                  >  1:476886/1‑140 (MQ=255)                                                                   aGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGcc                                                                  >  2:933204/1‑140 (MQ=255)                                                                   aGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGcc                                                                  >  2:627039/1‑140 (MQ=255)                                                                   aGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGc                                                                   <  2:211035/139‑1 (MQ=255)                                                                   aGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGc                                                                   >  1:211035/1‑139 (MQ=255)                                                                    gCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCATCCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGccc                                                                 <  2:357202/140‑1 (MQ=255)                                                                       tGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATGCAGCGCATCTTGTACGCCCCAg                                                              >  1:737720/1‑140 (MQ=255)                                                                                  gCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCgg                                                   >  1:349528/1‑140 (MQ=255)                                                                                   ccAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGc                                                  >  2:889489/1‑140 (MQ=255)                                                                                                tCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCAt                                     >  1:830487/1‑140 (MQ=255)                                                                                                    gTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGt                                              <  2:545848/127‑1 (MQ=255)                                                                                                    gTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGt                                              >  1:545848/1‑127 (MQ=255)                                                                                                    gTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACg                                 >  2:463728/1‑140 (MQ=255)                                                                                                            gATCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTtcgt                         <  1:646684/140‑1 (MQ=255)                                                                                                             aTCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTtcgtc                        <  1:627039/140‑1 (MQ=255)                                                                                                             aTCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTtcgt                         <  1:769894/139‑1 (MQ=255)                                                                                                             aTCACCACATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTtcgt                         >  2:769894/1‑139 (MQ=255)                                                                                                               caccacATTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTcgtcgt                      >  1:266426/1‑140 (MQ=255)                                                                                                                    caTTCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATaa                 <  2:830487/140‑1 (MQ=255)                                                                                                                       tCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGc              <  1:958576/140‑1 (MQ=255)                                                                                                                       tCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGc              >  1:290483/1‑140 (MQ=255)                                                                                                                       tCAGCAGCCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGc              >  1:434330/1‑140 (MQ=255)                                                                                                                         agcagcCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCtt            >  2:545877/1‑140 (MQ=255)                                                                                                                            agcCCGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAg         <  1:463728/140‑1 (MQ=255)                                                                                                                                cGGCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACg     >  1:356281/1‑140 (MQ=255)                                                                                                                                  gCAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGc   <  2:349528/140‑1 (MQ=255)                                                                                                                                   cAACCGCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCa  <  2:356281/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCA  >  NC_000913/241169‑241438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑