Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,303,164	G→A	R112C (CGC→TGC)	napF ←	ferredoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,303,164	0	G	A	100.0%	172.1 / NA	50	R112C (CGC→TGC)	napF	ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/23);  total (27/23)

ATGGCTGATACCGGACAGCTGGCTGCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCGGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAGGCGTAACAGAAGCTGCA  >  NC_000913/2303031‑2303293                                                                                                                                      |                                                                                                                                  aTGGCTGATACCGGACAGCTGGCTGCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCa                                                                                                                             <  1:917947/140‑1 (MQ=255)  tGGCTGATACCGGACAGCTGGCTGCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCAt                                                                                                                            >  1:83180/1‑140 (MQ=255)    gCTGATACCGGACAGCTGGCTGCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTc                                                                                                                          >  2:1079992/1‑140 (MQ=255)        aTACCGGACAGCTGGCTGCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAact                                                                                                                      >  2:213086/1‑140 (MQ=255)        aTACCGGACAGCTGGCTGCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAact                                                                                                                      <  2:480831/140‑1 (MQ=255)              gACAGCTGGCTGCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAActgactga                                                                                                                >  2:414415/1‑140 (MQ=255)                   ctggctgCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGc                                                                                                           >  2:336941/1‑140 (MQ=255)                     ggctgCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCa                                                                                                         >  1:143315/1‑140 (MQ=255)                      gctgCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAg                                                                                                        >  2:809143/1‑140 (MQ=255)                        tgCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGc                                                                                                      >  1:728784/1‑140 (MQ=255)                         gCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGAAACGCCAGGCa                                                                                                     >  1:758161/1‑140 (MQ=255)                            caTGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCAcgc                                                                                                  >  1:589332/1‑140 (MQ=255)                             aTGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCAcgcg                                                                                                 >  1:409180/1‑140 (MQ=255)                                           ttGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGaa                                                                                   >  2:167454/1‑140 (MQ=255)                                           ttGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGaa                                                                                   >  2:1011028/1‑140 (MQ=255)                                            tGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAAc                                                                                  >  2:65553/1‑140 (MQ=255)                                                aaGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCa                                                                              <  1:445527/140‑1 (MQ=255)                                                aaGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCa                                                                              <  1:1079992/140‑1 (MQ=255)                                                      gCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATccc                                                                        <  2:589332/140‑1 (MQ=255)                                                         aTTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAgg                                                                     <  2:340486/140‑1 (MQ=255)                                                                  cGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCa                                                                              <  2:778157/122‑1 (MQ=255)                                                                  cGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCa                                                                              >  1:778157/1‑122 (MQ=255)                                                                     cTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGgc                                                         <  2:83180/140‑1 (MQ=255)                                                                       ggTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGgcgc                                                       >  2:174907/1‑140 (MQ=255)                                                                          aGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGga                                                    <  2:409180/140‑1 (MQ=255)                                                                          aGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGga                                                    <  2:333224/140‑1 (MQ=255)                                                                          aGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGga                                                    <  1:167454/140‑1 (MQ=255)                                                                               ccGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGaaaa                                               >  1:460867/1‑140 (MQ=255)                                                                                  gagaGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAg                                            >  1:1046951/1‑140 (MQ=255)                                                                                  gagaGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAg                                            >  2:329597/1‑140 (MQ=255)                                                                                  gagaGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAg                                            >  2:144218/1‑140 (MQ=255)                                                                                       cGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGgc                                                         >  2:744860/1‑122 (MQ=255)                                                                                       cGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGgc                                                         <  1:744860/122‑1 (MQ=255)                                                                                       cGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGAtt                                       <  1:1011028/140‑1 (MQ=255)                                                                                       cGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGAtt                                       <  1:794201/140‑1 (MQ=255)                                                                                       cGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGAtt                                       <  2:728784/140‑1 (MQ=255)                                                                                                   gATAATTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCt                           <  1:213086/140‑1 (MQ=255)                                                                                                       aTTGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTgcgc                       <  1:754969/140‑1 (MQ=255)                                                                                                        ttGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTgcgcg                      <  2:481040/140‑1 (MQ=255)                                                                                                        ttGCCATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTgcgcg                      <  2:873515/140‑1 (MQ=255)                                                                                                           ccATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAg                   <  2:143315/140‑1 (MQ=255)                                                                                                            cATTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAgg                  <  2:460867/140‑1 (MQ=255)                                                                                                             aTTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAGGc                 >  2:462132/1‑140 (MQ=255)                                                                                                             aTTGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCCTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGAGGCAAGCCTGCGCGCAGGc                 >  2:11100/1‑140 (MQ=255)                                                                                                              ttGGCTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAGGCg                >  2:523564/1‑140 (MQ=255)                                                                                                                  cTCACAGCTATCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAGGCGTAAc            <  1:336941/140‑1 (MQ=255)                                                                                                                           aTCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAGGCGTAACAGAAGCTGc   >  2:320896/1‑140 (MQ=255)                                                                                                                           aTCCTGACAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAGGCGTAACAGAAGCTGc   >  1:655939/1‑140 (MQ=255)                                                                                                                                 aCAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCC‑ATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAGGCGTAACAGAAGCTGCa  >  1:300841/1‑134 (MQ=255)                                                                                                                                 aCAGCAGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCC‑ATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAGGCGTAACAGAAGCTGCa  <  2:300841/134‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  ATGGCTGATACCGGACAGCTGGCTGCACATGCTCCACATCCGTTGCAAAGTTGGCTATTAAGTTGCGGCTGGTAGATCCCGGAGAGCGTCGGGCGAAAGATAATTGCCATTGGCTCACAGCTATCCTGACAGCGGCGGCATTCAACTGACTGATACGCCAGGCACGCGTCCCCGATGGTGAACTGCAAATCCCAGGCCCTGGTGTGGCGCGGAGAAAATAGCGATTCGGGGCAAGCCTGCGCGCAGGCGTAACAGAAGCTGCA  >  NC_000913/2303031‑2303293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑