Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,331,932	T→C	pseudogene (2471/4605 nt)	yfaS ←	putative macroglobulin family protein, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,331,932	0	T	C	100.0%	118.9 / NA	33	pseudogene (2471/4605 nt)	yfaS	putative macroglobulin family protein, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/20);  total (13/20)

TTCACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGTTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAGGTTTACCTTTCAGCGACGAGGTTAATT  >  NC_000913/2331802‑2332064                                                                                                                                   |                                                                                                                                     ttCACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCa                                                                                                                             >  2:111318/1‑140 (MQ=255)   cACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGccc                                                                                                                               >  2:247933/1‑136 (MQ=255)   cACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGccc                                                                                                                               <  1:247933/136‑1 (MQ=255)           ggACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGaa                                                                                                                   >  2:361295/1‑140 (MQ=255)               gTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAAt                                                                                                               <  1:1068988/140‑1 (MQ=255)                ttCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAAtt                                                                                                              <  1:361295/140‑1 (MQ=255)                               tCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGgcg                                                                                               <  2:394688/140‑1 (MQ=255)                                   cgctcgctGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGAt                                                                                           >  1:554988/1‑140 (MQ=255)                                       cgctGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCt                                                                                       >  2:208842/1‑140 (MQ=255)                                              gTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCa                                                                                <  1:64404/140‑1 (MQ=255)                                                  gTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAgcgc                                                                            <  1:1039102/140‑1 (MQ=255)                                                  gTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAgcgc                                                                            <  1:691470/140‑1 (MQ=255)                                                   ttGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGcgcg                                                                           <  1:410090/140‑1 (MQ=255)                                                                     gCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGAcc                                                         >  2:342651/1‑140 (MQ=255)                                                                         gCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGc                                                     <  1:342651/140‑1 (MQ=255)                                                                         gCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGc                                                     <  1:219824/140‑1 (MQ=255)                                                                         gCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGc                                                     <  1:208842/140‑1 (MQ=255)                                                                          cTGGAGAGCGCCTGGTAGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACAcc                                                    >  2:345676/1‑140 (MQ=255)                                                                                    ccTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGAc                                                          <  1:269700/124‑1 (MQ=255)                                                                                    ccTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGAc                                                          >  2:269700/1‑124 (MQ=255)                                                                                             aGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGaa                                 <  2:338278/140‑1 (MQ=255)                                                                                              gCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGaaa                                <  2:296552/140‑1 (MQ=255)                                                                                              gCTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGaaa                                <  1:111318/140‑1 (MQ=255)                                                                                               cTGATAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAAc                               <  1:217396/140‑1 (MQ=255)                                                                                                  aTAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAgg                            >  1:1063447/1‑140 (MQ=255)                                                                                                  aTAAACGACAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAgg                            >  1:198485/1‑140 (MQ=255)                                                                                                         aCAGACTGGAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAGGTTTACCt                     >  2:1073229/1‑140 (MQ=255)                                                                                                                ggAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAGGTTTACCTTTCAGcg              <  2:198485/140‑1 (MQ=255)                                                                                                                ggAGCTGGTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAGGTTTACCTTTCAGcg              <  2:1063447/140‑1 (MQ=255)                                                                                                                       gTACGCACATTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACACCTACCGTTAGCTGCGCAGAAACAGGTTTACCTTTCAGCGACGAGGt       <  1:345676/140‑1 (MQ=255)                                                                                                                            cacaTTGCTACGCCCCAGCGGATAGAAAAATTTGCTGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAGGTTTACCTTTCAGCGACGAGGTTAAtt  >  1:367540/1‑140 (MQ=255)                                                                                                                            cacaTTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAGGTTTACCTTTCAGCGACGAGGTTAAtt  >  2:490829/1‑140 (MQ=255)                                                                                                                            cacaTTGCTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAGGTTTACCTTTCAGCGACGAGGTTAAtt  <  2:554988/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                     TTCACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGTTACGCCCCAGCGGATAGAAAAATTTGCCGATATTCGGCGCGATTTCTGGTTGCAGCGCGTAGATCATTTCATCGACCACGCCTACCGTTAGCTGCGCAGAAACAGGTTTACCTTTCAGCGACGAGGTTAATT  >  NC_000913/2331802‑2332064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑