Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,372,752	G→A	A66T (GCT→ACT)	arnE →	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnE subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,372,752	0	G	A	100.0%	247.1 / NA	71	A66T (GCT→ACT)	arnE	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnE subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/36);  total (35/36)

AGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGG  >  NC_000913/2372615‑2372887                                                                                                                                          |                                                                                                                                        aGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACt                                                                                                                                       <  1:541619/140‑1 (MQ=255)    accAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCAttacttac                                                                                                                                    <  1:276426/138‑1 (MQ=255)     aCAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTAcc                                                                                                                                   <  1:94729/140‑1 (MQ=255)           aaCCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGt                                                                                                                             <  1:1011866/140‑1 (MQ=255)                       ggCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAAtt                                                                                                                 >  2:65465/1‑140 (MQ=255)                           aTAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGt                                                                                                             >  1:699854/1‑140 (MQ=255)                              aacaaacGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTg                                                                                                          >  1:235008/1‑140 (MQ=255)                                caaacGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTggg                                                                                                        >  2:405208/1‑140 (MQ=255)                                caaacGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTggg                                                                                                        <  2:608706/140‑1 (MQ=255)                                     gtcgcAAACATATCGTGCTGCGGCTGGGACTGGCGCTGGCATGGCTTGGTCTTGCCATGGAGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGCAGGCATTACTTACCCGCTGTTTAGTCTGAATTTTGTCTGGGTGACg                                                                                                   >  2:1082528/3‑140 (MQ=255)                                       cgcAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgct                                                                                                 >  2:566514/1‑140 (MQ=255)                                          aaaCATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctggc                                                                                              <  2:699854/140‑1 (MQ=255)                                          aaaCATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGCAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctggc                                                                                              <  2:426187/140‑1 (MQ=255)                                            aCATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctggctg                                                                                            <  1:529300/140‑1 (MQ=255)                                               tatCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAg                                                                                         <  2:869210/140‑1 (MQ=255)                                                 tCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTa                                                                                       >  1:500607/1‑140 (MQ=255)                                                 tCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTa                                                                                       >  1:641584/1‑140 (MQ=255)                                                  cGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTaa                                                                                      <  2:850547/140‑1 (MQ=255)                                                    tGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTaaaa                                                                                    <  2:235008/140‑1 (MQ=255)                                                    tGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTaaaa                                                                                    >  1:223733/1‑140 (MQ=255)                                                      cTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGAACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACt                                                                                  >  1:782958/1‑140 (MQ=255)                                                        gtgGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACtgt                                                                                >  1:815302/1‑140 (MQ=255)                                                         tgGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACtgtg                                                                               >  1:384284/1‑140 (MQ=255)                                                            cTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCa                                                                            <  2:223733/140‑1 (MQ=255)                                                            cTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCa                                                                            <  2:815302/140‑1 (MQ=255)                                                              gggACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACg                                                                          >  1:622185/1‑140 (MQ=255)                                                                   tGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCg                                                                     >  1:954447/1‑140 (MQ=255)                                                                     gcgcTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACtg                                                                                 >  2:224950/1‑126 (MQ=255)                                                                     gcgcTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACtg                                                                                 <  1:224950/126‑1 (MQ=255)                                                                     gcgcTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGt                                                                   >  1:375862/1‑140 (MQ=255)                                                                     gcgcTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGt                                                                   >  1:326937/1‑140 (MQ=255)                                                                          ggCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATcgc                                                              >  1:825330/1‑140 (MQ=255)                                                                            cTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATcgccg                                                            <  2:638693/140‑1 (MQ=255)                                                                               gtcttggtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATcgccgc                                                           <  2:658797/138‑1 (MQ=255)                                                                               gtcttggtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATcgccgc                                                           >  1:658797/1‑138 (MQ=255)                                                                               gtcttggtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGt                                                         <  2:408649/140‑1 (MQ=255)                                                                                tcttggtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTc                                                        >  2:636385/1‑140 (MQ=255)                                                                                 cttggtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa                                                       <  1:405208/140‑1 (MQ=255)                                                                                 cttggtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa                                                       <  2:500607/140‑1 (MQ=255)                                                                                 cttggtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa                                                       <  2:641584/140‑1 (MQ=255)                                                                                 cttggtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa                                                       <  1:605857/140‑1 (MQ=255)                                                                                     gtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTgg                                                   >  2:940958/1‑140 (MQ=255)                                                                                     gtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTgg                                                   >  2:530885/1‑140 (MQ=255)                                                                                     gtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTgg                                                   >  1:723010/1‑140 (MQ=255)                                                                                      gcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGgt                                                  <  2:104342/139‑1 (MQ=255)                                                                                          gCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCcgcg                                                          <  1:984475/128‑1 (MQ=255)                                                                                          gCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa                                                       <  2:130042/131‑1 (MQ=255)                                                                                          gCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa                                                       >  1:130042/1‑131 (MQ=255)                                                                                          gCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCAc                                                      >  2:859155/1‑132 (MQ=255)                                                                                          gCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCAc                                                      <  1:859155/132‑1 (MQ=255)                                                                                          gCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTAAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCcgcg                                                          >  2:984475/1‑128 (MQ=255)                                                                                           ccATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTggg                                             >  2:1081594/1‑140 (MQ=255)                                                                                            cATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTgggg                                            <  1:936340/140‑1 (MQ=255)                                                                                             aTGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTA‑CCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTg                                          <  1:1040997/140‑1 (MQ=255)                                                                                              tGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTg                                          <  2:865601/140‑1 (MQ=255)                                                                                                gTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGc                                        <  1:1081594/140‑1 (MQ=255)                                                                                                      tGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCat                                  >  2:92844/1‑140 (MQ=255)                                                                                                        gctgctGGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCatta                                >  2:800607/1‑140 (MQ=255)                                                                                                            ctgGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTgg                            <  2:727301/140‑1 (MQ=255)                                                                                                            ctgGTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTgg                            <  1:92844/140‑1 (MQ=255)                                                                                                              ggTCTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTggcg                          <  1:800607/140‑1 (MQ=255)                                                                                                                 cTTGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCa                       <  2:384284/140‑1 (MQ=255)                                                                                                                  ttGCAGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCAt                      >  2:325962/1‑140 (MQ=255)                                                                                                                      aGAACGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATtgtg                  <  2:326937/140‑1 (MQ=255)                                                                                                                         aCGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATc               >  1:1048072/1‑140 (MQ=255)                                                                                                                          cGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTggcg                          <  1:485706/128‑1 (MQ=255)                                                                                                                          cGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTggcg                          >  2:485706/1‑128 (MQ=255)                                                                                                                          cGTACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATcc              >  1:129450/1‑140 (MQ=255)                                                                                                                            tACCGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCCGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTAGTGGCGGCATTCTGATCCTc            >  2:998831/1‑140 (MQ=255)                                                                                                                              ccGGTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCgg          <  1:1082528/140‑1 (MQ=255)                                                                                                                                ggTAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGa        <  1:940958/140‑1 (MQ=255)                                                                                                                                  tAGGCATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGt      >  2:364188/1‑140 (MQ=255)                                                                                                                                      cATTACTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACgg  >  1:933025/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGG  >  NC_000913/2372615‑2372887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑