Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,379,740	T→C	T302A (ACG→GCG)	menF ←	isochorismate synthase MenF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,379,740	0	T	C	100.0%	190.1 / NA	54	T302A (ACG→GCG)	menF	isochorismate synthase MenF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/33);  total (21/33)

CAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATT  >  NC_000913/2379608‑2379863                                                                                                                                     |                                                                                                                            cAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTa                                                                                                                      <  2:408480/140‑1 (MQ=255)  aaCTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTAt                                                                                                                     <  2:101433/140‑1 (MQ=255)   aCTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATc                                                                                                                    <  1:363812/140‑1 (MQ=255)       ctgcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGcc                                                                                                                >  2:824160/1‑140 (MQ=255)         gcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTg                                                                                                              <  2:180999/140‑1 (MQ=255)           cGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTa                                                                                                            <  2:811488/140‑1 (MQ=255)                 cTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGtt                                                                                                      >  1:219259/1‑140 (MQ=255)                  tGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTg                                                                                                     <  1:986416/140‑1 (MQ=255)                          aTGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGatat                                                                                             >  2:306451/1‑140 (MQ=255)                           tGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATc                                                                                            >  1:227340/1‑140 (MQ=255)                                   gATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccacca                                                                                    <  1:767123/140‑1 (MQ=255)                                    aTCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccaccac                                                                                   >  1:191313/1‑140 (MQ=255)                                    aTCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccaccac                                                                                   >  1:405748/1‑140 (MQ=255)                                    aTCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccaccac                                                                                   >  1:1476/1‑140 (MQ=255)                                    aTCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccaccac                                                                                   >  2:141765/1‑140 (MQ=255)                                    aTCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccaccac                                                                                   >  1:685855/1‑140 (MQ=255)                                       acaTCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAAAATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccaccaccac                                                                                >  1:538012/1‑139 (MQ=255)                                         atcatcCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccac                                                                                      >  1:53859/1‑132 (MQ=255)                                         atcatcCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccac                                                                                      <  2:53859/132‑1 (MQ=255)                                            atcCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGt                                                                           >  2:745239/1‑140 (MQ=255)                                              ccGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtc                                                                         >  2:853716/1‑140 (MQ=255)                                              ccGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtc                                                                         >  1:752024/1‑140 (MQ=255)                                               cGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtct                                                                        >  2:122875/1‑140 (MQ=255)                                                    tgttgAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCgcgc                                                                   <  1:824160/140‑1 (MQ=255)                                                     gttgAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCt                                                                  >  2:914520/1‑140 (MQ=255)                                                           tGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATTCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGtttt                                                            >  2:46906/1‑140 (MQ=255)                                                           tGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGtttt                                                            >  1:954370/1‑140 (MQ=255)                                                                 ccAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTatcat                                                      <  1:122875/140‑1 (MQ=255)                                                                  cAGATACAGCGGCGAAGATGCTGCCCTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTatcatc                                                     <  2:611214/140‑1 (MQ=255)                                                                  cAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTatcatc                                                     <  2:191313/140‑1 (MQ=255)                                                                    gATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCg                                                   <  2:193536/140‑1 (MQ=255)                                                                           cggcgAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAg                                            <  1:745239/140‑1 (MQ=255)                                                                             gcgAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGcc                                          <  2:685855/140‑1 (MQ=255)                                                                             gcgAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGcc                                          <  2:313401/140‑1 (MQ=255)                                                                              cgAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCa                                         <  1:891800/140‑1 (MQ=255)                                                                              cgAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCa                                         <  1:853716/140‑1 (MQ=255)                                                                              cgAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCa                                         <  2:1476/140‑1 (MQ=255)                                                                                aaGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCAct                                       <  1:522983/140‑1 (MQ=255)                                                                                aaGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCAct                                       <  1:46906/140‑1 (MQ=255)                                                                                                 gcagacgcagTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGcc                      <  2:954370/140‑1 (MQ=255)                                                                                                 gcagacgcagTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGcc                      <  1:100531/140‑1 (MQ=255)                                                                                                 gcagacgcagTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGcc                      <  2:971709/140‑1 (MQ=255)                                                                                                 gcagacgcagTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGcc                      <  1:914520/140‑1 (MQ=255)                                                                                                      cgcagTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCtt                 <  2:757485/140‑1 (MQ=255)                                                                                                         agTACCTGCGGCGGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTatc              <  2:405748/140‑1 (MQ=255)                                                                                                                gcggcgGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGa       <  2:219259/140‑1 (MQ=255)                                                                                                                gcggcgGTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGa       <  2:1095324/140‑1 (MQ=255)                                                                                                                 cggcggTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGat      >  2:18479/1‑140 (MQ=255)                                                                                                                  ggcggTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGatt     <  1:306451/140‑1 (MQ=255)                                                                                                                  ggcggTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGatt     <  1:18479/140‑1 (MQ=255)                                                                                                                   gcggTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGcc                      >  1:759110/1‑122 (MQ=255)                                                                                                                   gcggTAAAACATCCAGCGCCTGGGCATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGTTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGcc                      <  2:759110/122‑1 (MQ=255)                                                                                                                     ggTAAAACATCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGattatt  >  2:82569/1‑140 (MQ=255)                                                                                                                              tCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTAtcatca          <  2:342226/123‑1 (MQ=255)                                                                                                                              tCCAGCGCCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTAtcatca          >  1:342226/1‑123 (MQ=255)                                                                                                                                     |                                                                                                                            CAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATT  >  NC_000913/2379608‑2379863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑