Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	244,046	C→T	A168A (GCC→GCT)	lpcA →	D‑sedoheptulose 7‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	244,046	0	C	T	100.0%	206.7 / NA	59	A168A (GCC→GCT)	lpcA	D‑sedoheptulose 7‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/36);  total (23/36)

TCTGCAAACGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCCGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCC  >  NC_000913/243912‑244179                                                                                                                                       |                                                                                                                                      tctGCAAACGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCg                                                                                                                                  >  1:592018/1‑140 (MQ=255)   tGCAAACGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGAcc                                                                                                                                   >  2:439802/1‑137 (MQ=255)   tGCAAACGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGAcc                                                                                                                                   <  1:439802/137‑1 (MQ=255)   tGCAAACGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCa                                                                                                                                <  2:1040186/140‑1 (MQ=255)    gCAAACGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCAt                                                                                                                               <  1:504165/140‑1 (MQ=255)    gCAAACGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCAt                                                                                                                               >  2:1020009/1‑140 (MQ=255)      aaaCGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTc                                                                                                                             >  1:562523/1‑140 (MQ=255)       aaCGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCa                                                                                                                            <  2:991284/140‑1 (MQ=255)        aCGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAg                                                                                                                           <  2:359212/140‑1 (MQ=255)           tGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGgag                                                                                                                        >  1:38468/1‑140 (MQ=255)             aTCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCa                                                                                                                                <  2:810951/130‑1 (MQ=255)             aTCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCa                                                                                                                                >  1:810951/1‑130 (MQ=255)             aTCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGAt                                                                                                                      <  1:1020009/140‑1 (MQ=255)             aTCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGAt                                                                                                                      <  2:562523/140‑1 (MQ=255)             aTCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGAt                                                                                                                      >  2:881824/1‑140 (MQ=255)                aaaGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTca                                                                                                                   >  2:1068436/1‑140 (MQ=255)                  aGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTcaca                                                                                                                 >  1:735126/1‑140 (MQ=255)                  aGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTcaca                                                                                                                 >  2:591042/1‑140 (MQ=255)                   gCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACAt                                                                                                                <  2:69262/140‑1 (MQ=255)                    cGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACAtt                                                                                                               >  2:457563/1‑140 (MQ=255)                    cGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACAtt                                                                                                               >  1:487212/1‑140 (MQ=255)                      aTCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGATAAGCTGGCCGCATTCAGGAGATTCACATTaa                                                                                                             >  2:2540/1‑140 (MQ=255)                       tCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTaaa                                                                                                            >  2:1089341/1‑140 (MQ=255)                        cGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAg                                                                                                           <  1:547881/140‑1 (MQ=255)                        cGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAg                                                                                                           <  2:369323/140‑1 (MQ=255)                        cGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAg                                                                                                           >  2:491949/1‑140 (MQ=255)                         gCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGt                                                                                                          <  2:38468/140‑1 (MQ=255)                         gCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGt                                                                                                          <  1:457563/140‑1 (MQ=255)                         gCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGt                                                                                                          <  1:689002/140‑1 (MQ=255)                         gCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGt                                                                                                          <  2:487212/140‑1 (MQ=255)                         gCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGt                                                                                                          <  1:881824/140‑1 (MQ=255)                         gCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGt                                                                                                          <  2:585847/140‑1 (MQ=255)                         gCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGt                                                                                                          <  2:990937/140‑1 (MQ=255)                           aGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGa                                                                                                        <  1:20231/140‑1 (MQ=255)                                cgcgTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCat                                                                                                   >  2:250301/1‑140 (MQ=255)                                  cgTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCa                                                                                                    <  2:992453/137‑1 (MQ=255)                                  cgTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCa                                                                                                    >  1:992453/1‑137 (MQ=255)                                    tGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATcc                                                                                               <  2:126480/140‑1 (MQ=255)                                     gagaAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCt                                                                                              >  1:1035057/1‑140 (MQ=255)                                                      gATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGaaa                                                                             <  1:175327/140‑1 (MQ=255)                                                           cccTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAagag                                                                        <  1:1089341/140‑1 (MQ=255)                                                           cccTGACCGGTAAAGACGGCAGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAagag                                                                        <  1:250301/140‑1 (MQ=255)                                                            ccTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAgaga                                                                       >  1:17126/1‑140 (MQ=255)                                                            ccTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAgaga                                                                       >  1:427685/1‑140 (MQ=255)                                                               gACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATgg                                                                    <  2:345453/140‑1 (MQ=255)                                                               gACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATgg                                                                    <  1:605134/140‑1 (MQ=255)                                                               gACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATgg                                                                    <  1:491949/140‑1 (MQ=255)                                                                aCCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGaaa                                                                             >  2:493442/1‑130 (MQ=255)                                                                aCCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGaaa                                                                             <  1:493442/130‑1 (MQ=255)                                                                    gTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTtaag                                                               <  2:735126/140‑1 (MQ=255)                                                                     tAAAGACGGGGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTtaagt                                                              <  1:776386/140‑1 (MQ=255)                                                                     tAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTtaagt                                                              >  1:422096/1‑140 (MQ=255)                                                                         gACGGCGGCAAAATGGCTGGCACGGTGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTtaagtaagc                                                          >  2:159207/1‑139 (MQ=255)                                                                         gACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTaagtaagt                                                          <  1:1068436/140‑1 (MQ=255)                                                                          aCGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTc                                                         >  1:412597/1‑140 (MQ=255)                                                                              cggcAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTtaag                                                               <  1:44140/130‑1 (MQ=255)                                                                              cggcAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTtaag                                                               >  2:44140/1‑130 (MQ=255)                                                                               ggcAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCg                                                    <  1:591042/140‑1 (MQ=255)                                                                                    aaTGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGc                                               <  1:2540/140‑1 (MQ=255)                                                                                                        tCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTAcgccg                           <  2:592018/140‑1 (MQ=255)                                                                                                        tCGAAATTCGCGTACCGCACTTTGGTTATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTAcgccg                           <  2:412597/140‑1 (MQ=255)                                                                                                                                 ttATGCTGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcc  >  1:670469/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      TCTGCAAACGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAAGTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATTCGCGTACCGCACTTTGGTTATGCCGACCGCATTCAGGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCC  >  NC_000913/243912‑244179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑