Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,460,707	G→A	I76I (ATC→ATT)	yfcZ ←	DUF406 domain‑containing protein YfcZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,460,707	0	G	A	100.0%	165.7 / NA	47	I76I (ATC→ATT)	yfcZ	DUF406 domain‑containing protein YfcZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/21);  total (26/21)

TGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGG  >  NC_000913/2460572‑2460842                                                                                                                                        |                                                                                                                                        tGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAatat                                                                                                                                     >  2:879762/1‑140 (MQ=255)  gACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATc                                                                                                                                    <  1:1090947/140‑1 (MQ=255)  gACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATc                                                                                                                                    <  1:879762/140‑1 (MQ=255)       agagagAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGgc                                                                                                                               >  1:868462/1‑140 (MQ=255)        gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGgcg                                                                                                                              >  1:1048438/1‑140 (MQ=255)        gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGgcg                                                                                                                              >  2:1008991/1‑140 (MQ=255)          gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGca                                                                                                                            <  1:1093805/140‑1 (MQ=255)            gaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGcaca                                                                                                                          <  2:609883/140‑1 (MQ=255)               aagaagaagAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGgcg                                                                                                                              >  2:355729/1‑133 (MQ=255)               aagaagaagAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGgcg                                                                                                                              <  1:355729/133‑1 (MQ=255)               aagaagaagAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCg                                                                                                                       >  2:499398/1‑140 (MQ=255)                   agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAg                                                                                                                   >  1:327813/1‑140 (MQ=255)                    gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGa                                                                                                                  <  1:507023/140‑1 (MQ=255)                     aagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGAc                                                                                                                 >  2:163849/1‑140 (MQ=255)                     aagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGAc                                                                                                                 >  2:1016021/1‑140 (MQ=255)                       gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGActc                                                                                                               <  1:1008991/140‑1 (MQ=255)                                 aaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa                                                                                                     >  2:963977/1‑140 (MQ=255)                                 aaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa                                                                                                     >  1:990476/1‑140 (MQ=255)                                 aaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa                                                                                                     >  1:642268/1‑140 (MQ=255)                                    gCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTc                                                                                                  >  2:365197/1‑140 (MQ=255)                                                aaCTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTg                                                                                      <  1:321282/140‑1 (MQ=255)                                                 aCTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGc                                                                                     <  1:762320/140‑1 (MQ=255)                                                 aCTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGc                                                                                     <  2:825383/140‑1 (MQ=255)                                                 aCTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGc                                                                                     <  1:163849/140‑1 (MQ=255)                                                  cTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCa                                                                                    <  2:455046/140‑1 (MQ=255)                                                   tGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCaa                                                                                   <  2:868462/140‑1 (MQ=255)                                                   tGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCaa                                                                                   >  2:195062/1‑140 (MQ=255)                                                      cAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGt                                                                                <  2:990476/140‑1 (MQ=255)                                                             gtacgtacCCGAGAGGAATTAACGCAGACCCAGCTGGAAAGTCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGAtt                                                                         >  2:493297/1‑140 (MQ=255)                                                                    cccGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCt                                                                  >  2:156389/1‑140 (MQ=255)                                                                    cccGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCt                                                                  >  1:238824/1‑140 (MQ=255)                                                                       gagaGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACg                                                               <  1:963977/140‑1 (MQ=255)                                                                        agagGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACgg                                                              >  1:747392/1‑140 (MQ=255)                                                                         gagGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACggg                                                             <  2:747392/140‑1 (MQ=255)                                                                               ttAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCttttt                                                       >  1:749132/1‑140 (MQ=255)                                                                               ttAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGATCAGATTCCACGCTACGGGCttttt                                                       >  2:256580/1‑140 (MQ=255)                                                                                    gCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTtcagtc                                                  >  2:23193/1‑140 (MQ=255)                                                                                      aGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTtcagtcag                                                <  2:1048438/140‑1 (MQ=255)                                                                                             gCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCTACGCTACGGGCTTTTTCAGTCAGCgccgcc                                         >  2:330217/1‑140 (MQ=255)                                                                                             gCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCgccgcc                                         <  1:365197/140‑1 (MQ=255)                                                                                                        aGCATTTCAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGctc                              >  2:966474/1‑140 (MQ=255)                                                                                                               cAGCTTCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCt                       >  2:906615/1‑140 (MQ=255)                                                                                                                   ttCGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTgcgc                   <  2:78076/140‑1 (MQ=255)                                                                                                                     cGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTgcg                    <  2:813163/137‑1 (MQ=255)                                                                                                                     cGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTgcg                    >  1:813163/1‑137 (MQ=255)                                                                                                                     cGCAGGCAAAAGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCgg                 >  1:287715/1‑140 (MQ=255)                                                                                                                               aGTGAAATCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAaca       >  2:834108/1‑140 (MQ=255)                                                                                                                                    aaTCAATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCgg  <  2:749132/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGG  >  NC_000913/2460572‑2460842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑