Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,823	T→C	V293A (GTG→GCG)	yfeH →	putative solute:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,823	0	T	C	100.0%	183.7 / NA	50	V293A (GTG→GCG)	yfeH	putative solute:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/31);  total (19/31)

CTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTC  >  NC_000913/2527689‑2527956                                                                                                                                       |                                                                                                                                      cTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGcgctgc                                                                                                                                  >  1:222467/1‑140 (MQ=255)  tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                                                 >  1:46293/1‑140 (MQ=255)  tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                                                 >  1:703293/1‑140 (MQ=255)        gCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgat                                                                                                                           <  2:846576/140‑1 (MQ=255)        gCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgat                                                                                                                           <  2:668560/140‑1 (MQ=255)        gCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTCCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACCTTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgat                                                                                                                           <  2:222467/140‑1 (MQ=255)            gCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGAtt                                                                                                                       >  2:800273/1‑140 (MQ=255)                  tGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCAt                                                                                                                 <  1:926610/140‑1 (MQ=255)                   gAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATc                                                                                                                <  2:375415/140‑1 (MQ=255)                     gCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAg                                                                                                              <  1:904922/140‑1 (MQ=255)                       ttCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGAt                                                                                                            <  1:311617/140‑1 (MQ=255)                       ttCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGAt                                                                                                            <  1:943071/140‑1 (MQ=255)                       ttCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGAt                                                                                                            <  1:800273/140‑1 (MQ=255)                              aGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTg                                                                                                     >  1:342776/1‑140 (MQ=255)                                gCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGAt                                                                                                   <  2:9081/140‑1 (MQ=255)                                gCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGAt                                                                                                   <  2:46293/140‑1 (MQ=255)                                   gATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGt                                                                                                <  2:652948/140‑1 (MQ=255)                                    aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                               >  2:311186/1‑140 (MQ=255)                                    aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                               >  1:686847/1‑140 (MQ=255)                                        aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                           <  2:750779/140‑1 (MQ=255)                                        aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                           <  1:153866/140‑1 (MQ=255)                                        aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                           <  2:703293/140‑1 (MQ=255)                                        aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                           <  2:907635/140‑1 (MQ=255)                                        aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTCCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                           <  1:572408/140‑1 (MQ=255)                                                  gTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTgg                                                                                  <  2:544497/139‑1 (MQ=255)                                                  gTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTgg                                                                                  >  1:544497/1‑139 (MQ=255)                                                   tCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGgcg                                                                                >  1:649758/1‑140 (MQ=255)                                                   tCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGgcg                                                                                >  2:1002911/1‑140 (MQ=255)                                                          tgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGAt                                                                         >  1:1053657/1‑140 (MQ=255)                                                          tgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGAt                                                                         >  1:455640/1‑140 (MQ=255)                                                          tgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGAt                                                                         >  1:59093/1‑140 (MQ=255)                                                            gTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATAc                                                                       <  1:55543/140‑1 (MQ=255)                                                                 aaaaaGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACg                                                                  >  2:293064/1‑140 (MQ=255)                                                                      gagTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGa                                                             <  2:507646/140‑1 (MQ=255)                                                                           tGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGaa                                                        >  1:473099/1‑140 (MQ=255)                                                                            ggCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAAc                                                       >  2:513442/1‑140 (MQ=255)                                                                                 aTGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGa                                                             <  2:959121/129‑1 (MQ=255)                                                                                 aTGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGa                                                             >  1:959121/1‑129 (MQ=255)                                                                                   ggCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACa                                                <  1:293064/140‑1 (MQ=255)                                                                                        cccGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcgc                                           <  1:784024/140‑1 (MQ=255)                                                                                        cccGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcgc                                           <  1:513442/140‑1 (MQ=255)                                                                                                      ttCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAagcagc                             <  2:1053657/140‑1 (MQ=255)                                                                                                        cTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAgcgc                           <  2:455640/140‑1 (MQ=255)                                                                                                            tCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGAt                       <  2:356135/140‑1 (MQ=255)                                                                                                                cacaTCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGAT‑‑‑‑ACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTa               >  1:955870/1‑140 (MQ=255)                                                                                                                   aTCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCtt                >  1:547115/1‑140 (MQ=255)                                                                                                                           tCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGTAAAGCAGCGCCGATAAAGCTTAAAGCGGa        <  2:342776/140‑1 (MQ=255)                                                                                                                           tCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa        <  1:311186/140‑1 (MQ=255)                                                                                                                           tCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa        <  1:1034180/140‑1 (MQ=255)                                                                                                                               tATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCt    >  2:820863/1‑140 (MQ=255)                                                                                                                                 tgatgGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTc  <  2:649758/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTC  >  NC_000913/2527689‑2527956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑