Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,701,467	G→A	P88L (CCA→CTA)	pdxJ ←	pyridoxine 5'‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,701,467	0	G	A	100.0%	164.3 / NA	47	P88L (CCA→CTA)	pdxJ	pyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/25);  total (22/25)

AACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACG  >  NC_000913/2701332‑2701601                                                                                                                                        |                                                                                                                                       aaCAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCtt                                                                                                                                    <  2:273250/140‑1 (MQ=255) aaCAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCtt                                                                                                                                    <  1:687062/140‑1 (MQ=255)      agaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGtct                                                                                                                               >  1:1056077/1‑140 (MQ=255)      agaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGAACCAGGCAGCAAAAATGTAGCTTCGtct                                                                                                                               >  2:734223/1‑140 (MQ=255)       gaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGtctc                                                                                                                              >  2:971752/1‑140 (MQ=255)       gaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGtctc                                                                                                                              >  2:810093/1‑140 (MQ=255)       gaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGtctc                                                                                                                              >  2:576943/1‑140 (MQ=255)        aaaCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCa                                                                                                                             >  2:797325/1‑140 (MQ=255)         aaCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCaa                                                                                                                            <  1:810093/140‑1 (MQ=255)                    cGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGcc                                                                                                                 <  1:699740/140‑1 (MQ=255)                      gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAg                                                                                                               <  1:971752/140‑1 (MQ=255)                             ccAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATctct                                                                                                        >  1:977607/1‑140 (MQ=255)                                    tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCa                                                                                                 >  1:452363/1‑140 (MQ=255)                                    tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCa                                                                                                 <  2:452363/140‑1 (MQ=255)                                      tGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCAcc                                                                                               >  2:988329/1‑140 (MQ=255)                                         aGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGcc                                                                                            <  1:576943/140‑1 (MQ=255)                                         aGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGcc                                                                                            <  1:26080/140‑1 (MQ=255)                                               cgcgCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTcc                                                                                      >  2:86047/1‑140 (MQ=255)                                               cgcgCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTcc                                                                                      >  1:1091090/1‑140 (MQ=255)                                               cgcgCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTcc                                                                                      >  2:236675/1‑140 (MQ=255)                                                          tCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCg                                                                           >  2:828432/1‑140 (MQ=255)                                                                  cccTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCa                                                                   >  2:137912/1‑140 (MQ=255)                                                                      gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGt                                                               <  2:657976/140‑1 (MQ=255)                                                                        gACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCt                                                             <  2:977607/140‑1 (MQ=255)                                                                            tCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACg                                                         >  1:9515/1‑140 (MQ=255)                                                                            tCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACg                                                         >  2:739811/1‑140 (MQ=255)                                                                              cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCCTCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCa                                                       <  2:1056077/140‑1 (MQ=255)                                                                                      cTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGGTgcgc                                               >  1:576231/1‑140 (MQ=255)                                                                                      cTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATgcgc                                               >  1:980656/1‑140 (MQ=255)                                                                                       ttCGGTTGTTACTTGCTGACGCTTTTCCGGCACCAGGCAGCAAAACTGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGAGGCGCa                                              >  2:816295/1‑140 (MQ=255)                                                                                           gttgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTc                                          <  2:551015/140‑1 (MQ=255)                                                                                             tgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                        <  1:988329/140‑1 (MQ=255)                                                                                             tgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                        <  2:625784/140‑1 (MQ=255)                                                                                              gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                       <  1:325490/140‑1 (MQ=255)                                                                                              gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                       <  1:426035/140‑1 (MQ=255)                                                                                                tACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                     <  2:563143/140‑1 (MQ=255)                                                                                                tACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                     <  2:698126/140‑1 (MQ=255)                                                                                                     ccTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTa                                >  2:305679/1‑140 (MQ=255)                                                                                                      cTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                        >  2:94548/1‑131 (MQ=255)                                                                                                      cTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                        <  1:94548/131‑1 (MQ=255)                                                                                                      cTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTaa                               >  1:934968/1‑140 (MQ=255)                                                                                                                tCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCg                     >  1:829656/1‑140 (MQ=255)                                                                                                                   ggTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATc                  <  1:737221/140‑1 (MQ=255)                                                                                                                               caAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg      <  1:797325/140‑1 (MQ=255)                                                                                                                               caAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg      >  1:189375/1‑140 (MQ=255)                                                                                                                               caAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg      <  1:137912/140‑1 (MQ=255)                                                                                                                                 aaaaTGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCa    <  2:9515/140‑1 (MQ=255)                                                                                                                                 aaaaTGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCa    <  2:1091090/140‑1 (MQ=255)                                                                                                                                   aaTGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACg  <  1:734223/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       AACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACG  >  NC_000913/2701332‑2701601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑