Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,732,254	T→C	K640K (AAA→AAG)	clpB ←	ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,254	0	T	C	100.0%	157.5 / NA	42	K640K (AAA→AAG)	clpB	ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/21);  total (21/21)

ACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGTTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGA  >  NC_000913/2732117‑2732389                                                                                                                                          |                                                                                                                                        aCATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGctt                                                                                                                                       <  2:1058951/140‑1 (MQ=255)   aTCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTtct                                                                                                                                     >  1:443564/1‑140 (MQ=255)     ccGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTcc                                                                                                                                   >  1:86103/1‑140 (MQ=255)     ccGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTcc                                                                                                                                   <  1:734249/140‑1 (MQ=255)     ccGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTcc                                                                                                                                   >  1:530315/1‑140 (MQ=255)            cgcTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACt                                                                                                                            >  2:585547/1‑140 (MQ=255)                ttttCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGa                                                                                                                        <  1:8900/140‑1 (MQ=255)                ttttCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGa                                                                                                                        <  2:774318/140‑1 (MQ=255)                ttttCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGa                                                                                                                        <  2:165671/140‑1 (MQ=255)                 tttCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGAc                                                                                                                       >  1:93745/1‑140 (MQ=255)                  ttCCACTTCATCCATCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACa                                                                                                                      <  2:570762/140‑1 (MQ=255)                   tCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACat                                                                                                                     <  1:846413/140‑1 (MQ=255)                       cTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCg                                                                                                                 <  2:697134/140‑1 (MQ=255)                         tCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGAt                                                                                                               >  1:975413/1‑140 (MQ=255)                                 cagGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGa                                                                                                                        <  2:750203/123‑1 (MQ=255)                                 cagGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGa                                                                                                                        >  1:750203/1‑123 (MQ=255)                                     aTGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGc                                                                                                   <  1:67131/140‑1 (MQ=255)                                      tGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATa                                                                                                              >  1:899900/1‑128 (MQ=255)                                      tGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATa                                                                                                              <  2:899900/128‑1 (MQ=255)                                      tGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGcc                                                                                                  >  2:1071588/1‑140 (MQ=255)                                      tGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGcc                                                                                                  >  2:141146/1‑140 (MQ=255)                                       gACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCt                                                                                                 <  2:779985/140‑1 (MQ=255)                                        aCGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCtc                                                                                                >  1:11083/1‑140 (MQ=255)                                              tACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCt                                                                                          >  2:119035/1‑140 (MQ=255)                                                        gacgCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATa                                                                                >  2:111738/1‑140 (MQ=255)                                                         acgCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATaa                                                                               <  1:585547/140‑1 (MQ=255)                                                                  ttCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCa                                                                      <  2:86103/140‑1 (MQ=255)                                                                  ttCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCa                                                                      <  2:530315/140‑1 (MQ=255)                                                                                 aCCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGct                                                       <  1:141146/140‑1 (MQ=255)                                                                                       ttcATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGttt                                                 >  1:900317/1‑140 (MQ=255)                                                                                                           gAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCg                             <  2:93745/140‑1 (MQ=255)                                                                                                           gAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCg                             <  2:900317/140‑1 (MQ=255)                                                                                                           gAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCg                             <  2:443564/140‑1 (MQ=255)                                                                                                           gAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCg                             <  1:119035/140‑1 (MQ=255)                                                                                                           gAGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTAGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCTAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCg                             >  2:759153/1‑140 (MQ=255)                                                                                                            aGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGa                            >  2:630492/1‑140 (MQ=255)                                                                                                            aGGCGCACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGa                            >  2:407868/1‑140 (MQ=255)                                                                                                              gcgcACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAgg                          <  1:111738/140‑1 (MQ=255)                                                                                                                 cACCAACCAAACGAGACACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAAc                       >  2:61957/1‑140 (MQ=255)                                                                                                                              agaCACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCaa          >  1:340960/1‑140 (MQ=255)                                                                                                                               gaCACCGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAAt         <  1:407868/140‑1 (MQ=255)                                                                                                                                    cGAGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGc    >  2:226371/1‑140 (MQ=255)                                                                                                                                      aGTGCTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGa  >  1:72195/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        ACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGACACCGAGTGTTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGA  >  NC_000913/2732117‑2732389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑