Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	253,476	T→C	pseudogene (10/267 nt)	ykfJ →	conserved protein YkfJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	253,476	0	T	C	100.0%	347.4 / NA	92	pseudogene (10/267 nt)	ykfJ	conserved protein YkfJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/46);  total (46/46)

ATACTCTAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGTACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGCGCGTAGTGGGGAT  >  NC_000913/253340‑253612                                                                                                                                         |                                                                                                                                         ataCTCTAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATGAATGGAATGGcaca                                                                                                                                       <  2:635142/140‑1 (MQ=255)     tctAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATggg                                                                                                                                   >  2:450355/1‑140 (MQ=255)     tctAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATggg                                                                                                                                   >  2:471821/1‑140 (MQ=255)     tctAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATggg                                                                                                                                   >  2:818534/1‑140 (MQ=255)      ctAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGc                                                                                                                                  >  2:351958/1‑140 (MQ=255)       tAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGcaca                                                                                                                                       <  1:878927/134‑1 (MQ=255)       tAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGcaca                                                                                                                                       >  2:878927/1‑134 (MQ=255)       tAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCa                                                                                                                                 >  2:955816/1‑140 (MQ=255)       tAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCa                                                                                                                                 >  1:892915/1‑140 (MQ=255)       tAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCa                                                                                                                                 >  1:527663/1‑140 (MQ=255)       tAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCa                                                                                                                                 >  1:431543/1‑140 (MQ=255)       tAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCa                                                                                                                                 >  2:746790/1‑140 (MQ=255)        aaaTAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCaa                                                                                                                                <  2:229892/140‑1 (MQ=255)         aataatTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCaaa                                                                                                                               <  1:818534/140‑1 (MQ=255)         aataatTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCaaa                                                                                                                               >  1:1036540/1‑140 (MQ=255)          ataatTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATggg                                                                                                                                   <  1:33667/135‑1 (MQ=255)          ataatTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATggg                                                                                                                                   >  2:33667/1‑135 (MQ=255)              ttCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAatatat                                                                                                                          >  1:623677/1‑140 (MQ=255)               tCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATAtt                                                                                                                         <  2:885516/140‑1 (MQ=255)                    ttGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTcc                                                                                                                    <  1:836444/140‑1 (MQ=255)                       cAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCtt                                                                                                                 >  1:116476/1‑140 (MQ=255)                               cGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATg                                                                                                         <  2:1008110/140‑1 (MQ=255)                               cGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATg                                                                                                         <  2:411822/140‑1 (MQ=255)                               cGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATg                                                                                                         >  2:696686/1‑140 (MQ=255)                               cGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATg                                                                                                         <  1:471821/140‑1 (MQ=255)                                 aCAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCg                                                                                                       <  1:351958/140‑1 (MQ=255)                                 aCAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCg                                                                                                       >  1:702861/1‑140 (MQ=255)                                 aCAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCg                                                                                                       <  2:1072720/140‑1 (MQ=255)                                 aCAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCg                                                                                                       <  1:450355/140‑1 (MQ=255)                                      cGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTAtt                                                                                                  >  1:143347/1‑140 (MQ=255)                                        aGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTa                                                                                                >  2:146098/1‑140 (MQ=255)                                                      aGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  <  2:431543/140‑1 (MQ=255)                                                      aGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  <  2:828012/140‑1 (MQ=255)                                                          tACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGAcc                                                                              <  2:527663/140‑1 (MQ=255)                                                          tACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGAcc                                                                              <  2:143347/140‑1 (MQ=255)                                                               aagtaagtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTgg                                                                         <  2:724199/140‑1 (MQ=255)                                                                agtaagtGACTGGGGTGAATGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  >  1:1033763/1‑130 (MQ=255)                                                                agtaagtGACTGGGGTGAATGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  <  2:1033763/130‑1 (MQ=255)                                                                 gtaagtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  <  1:315806/129‑1 (MQ=255)                                                                 gtaagtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  >  2:315806/1‑129 (MQ=255)                                                                 gtaagtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGAt                                                                       >  2:510706/1‑140 (MQ=255)                                                                 gtaagtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGAt                                                                       <  2:892915/140‑1 (MQ=255)                                                                  taagtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATc                                                                      <  1:955816/140‑1 (MQ=255)                                                                   aagtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  <  1:519602/127‑1 (MQ=255)                                                                   aagtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  >  2:519602/1‑127 (MQ=255)                                                                    agtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  <  1:1057728/126‑1 (MQ=255)                                                                    agtGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCtgat                                                                                  >  2:1057728/1‑126 (MQ=255)                                                                      tGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCgaga                                                                  >  2:459956/1‑140 (MQ=255)                                                                      tGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGGGGATCgaga                                                                  >  2:521108/1‑140 (MQ=255)                                                                          tGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCga                                                                    <  2:410289/134‑1 (MQ=255)                                                                          tGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCga                                                                    >  1:410289/1‑134 (MQ=255)                                                                           ggggTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTgg                                                                         >  2:451740/1‑128 (MQ=255)                                                                           ggggTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTgg                                                                         <  1:451740/128‑1 (MQ=255)                                                                            gggTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAg                                                            >  1:506158/1‑140 (MQ=255)                                                                                  acgaacgCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAg                                                            <  1:707978/134‑1 (MQ=255)                                                                                  acgaacgCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAg                                                            >  2:707978/1‑134 (MQ=255)                                                                                  acgaacgCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATcc                                                      <  1:696686/140‑1 (MQ=255)                                                                                    gaacgCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCcaa                                                    <  2:1036540/140‑1 (MQ=255)                                                                                    gaacgCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCcaa                                                    <  2:623677/140‑1 (MQ=255)                                                                                       cgcagtcgcagtACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCcaac                                                   <  1:102927/138‑1 (MQ=255)                                                                                       cgcagtcgcagtACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCcaac                                                   >  2:102927/1‑138 (MQ=255)                                                                                           gtcgcagtACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTac                                             >  1:760356/1‑140 (MQ=255)                                                                                           gtcgcagtACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTa                                              >  1:845175/1‑139 (MQ=255)                                                                                           gtcgcagtACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTa                                              <  2:845175/139‑1 (MQ=255)                                                                                            tcgcagtACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATcc                                                      >  2:780990/1‑130 (MQ=255)                                                                                            tcgcagtACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATcc                                                      <  1:780990/130‑1 (MQ=255)                                                                                            tcgcagtACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTaca                                            >  2:742723/1‑140 (MQ=255)                                                                                                  tACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACAcaccac                                      >  1:596969/1‑140 (MQ=255)                                                                                                    cATGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACgg                                    <  1:746790/140‑1 (MQ=255)                                                                                                      tGCAACTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCa                                  >  1:804574/1‑140 (MQ=255)                                                                                                         aaCTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAAt                               <  2:180867/140‑1 (MQ=255)                                                                                                          aCTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAAtt                              >  1:390447/1‑140 (MQ=255)                                                                                                          aCTTGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAAtt                              <  2:714213/140‑1 (MQ=255)                                                                                                            ttGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTa                            >  1:1075536/1‑140 (MQ=255)                                                                                                            ttGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTa                            >  1:446932/1‑140 (MQ=255)                                                                                                             tGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTAc                           <  1:395334/140‑1 (MQ=255)                                                                                                             tGAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTAc                           <  2:702861/140‑1 (MQ=255)                                                                                                              gAAGTATGACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTAcc                          >  1:447463/1‑140 (MQ=255)                                                                                                                     gACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATg                   <  2:804574/140‑1 (MQ=255)                                                                                                                     gACGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATg                   <  1:459956/140‑1 (MQ=255)                                                                                                                      aCGAGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGc                  <  2:1075536/140‑1 (MQ=255)                                                                                                                         aGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAgc               <  2:506158/140‑1 (MQ=255)                                                                                                                         aGTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAgc               >  1:876768/1‑140 (MQ=255)                                                                                                                          gTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAgcg              >  1:682881/1‑140 (MQ=255)                                                                                                                          gTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAgcg              <  2:603682/140‑1 (MQ=255)                                                                                                                          gTATAAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAgcg              <  2:116476/140‑1 (MQ=255)                                                                                                                            ataAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGcgcg            >  1:392279/1‑140 (MQ=255)                                                                                                                            ataAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGcgcg            >  1:1028682/1‑140 (MQ=255)                                                                                                                            ataAATGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGcgcg            <  1:510706/140‑1 (MQ=255)                                                                                                                              aaaTGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGCGCGTa          <  2:682881/140‑1 (MQ=255)                                                                                                                              aaaTGGAATGGCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGCGCGTa          <  2:1028682/140‑1 (MQ=255)                                                                                                                                atggaatggCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGCGCGTAGt        >  1:1062707/1‑140 (MQ=255)                                                                                                                                     atggCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCGTCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGCGCGTAGTGGGGa   >  2:103130/1‑140 (MQ=255)                                                                                                                                     atggCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGCGCGTAGTGGGGa   >  1:1087478/1‑140 (MQ=255)                                                                                                                                      tggCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGCGCGTAGTGGGGAt  >  2:568424/1‑140 (MQ=255)                                                                                                                                      tggCACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGCGCGTAGTGGGGAt  <  2:1083482/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATACTCTAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCGCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGGAATGGTACATGGGCAAATATATTCGTCCCTTATCCGATGCGGTATTTACCATCGCATCTGATGACCTGTGGATCGAGAGTTTAGCGATCCAACAATTACACACCACGGCAAATTTACCCAACATGCAGCGCGTAGTGGGGAT  >  NC_000913/253340‑253612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑