Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,971,266	A→G	intergenic (+133/‑5)	ygdQ → / → ygdR	UPF0053 inner membrane protein YgdQ/DUF903 domain‑containing lipoprotein YgdR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,971,266	0	A	G	100.0%	259.2 / NA	69	intergenic (+133/‑5)	ygdQ/ygdR	UPF0053 inner membrane protein YgdQ/DUF903 domain‑containing lipoprotein YgdR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/23);  total (46/23)

CGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAACCTGA  >  NC_000913/2971139‑2971392                                                                                                                                |                                                                                                                               cGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCg                                                                                                                             <  2:80979/131‑1 (MQ=255) cGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCg                                                                                                                             >  1:80979/1‑131 (MQ=255)   tACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAAt                                                                                                                  >  1:904102/1‑140 (MQ=255)   tACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAAt                                                                                                                  >  1:322068/1‑140 (MQ=255)      gCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATggg                                                                                                               <  1:79461/140‑1 (MQ=255)      gCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATggg                                                                                                               <  2:4927/140‑1 (MQ=255)        tctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGct                                                                                                             >  1:103973/1‑139 (MQ=255)          tcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGt                                                                                                           >  2:167190/1‑140 (MQ=255)           ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGta                                                                                                          >  1:171400/1‑140 (MQ=255)           ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGta                                                                                                          <  1:208646/140‑1 (MQ=255)             tGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGtaat                                                                                                        >  1:238113/1‑140 (MQ=255)                    gggCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCt                                                                                                 >  1:593005/1‑140 (MQ=255)                    gggCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCt                                                                                                 >  2:445007/1‑140 (MQ=255)                    gggCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCt                                                                                                 >  2:735831/1‑140 (MQ=255)                    gggCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCt                                                                                                 >  2:775780/1‑140 (MQ=255)                      gCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGc                                                                                               >  2:129832/1‑140 (MQ=255)                        gTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAg                                                                                             <  1:784976/140‑1 (MQ=255)                                tcCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTg                                                                                     >  2:401685/1‑140 (MQ=255)                                     ttACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGtt                                                                                >  1:477455/1‑140 (MQ=255)                                      tACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGttt                                                                               >  1:828598/1‑140 (MQ=255)                                         ccTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCt                                                                            <  2:633869/140‑1 (MQ=255)                                          cTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTg                                                                           <  2:593005/140‑1 (MQ=255)                                          cTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTg                                                                           >  2:118612/1‑140 (MQ=255)                                          cTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTg                                                                           >  1:1080534/1‑140 (MQ=255)                                            cacaGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGtt                                                                         >  1:595449/1‑140 (MQ=255)                                            cacaGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGtt                                                                         >  1:1051352/1‑140 (MQ=255)                                            cacaGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGtt                                                                         >  1:539582/1‑140 (MQ=255)                                                 tttAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCgg                                                                    <  1:519742/140‑1 (MQ=255)                                                  ttAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCggg                                                                   <  2:828598/140‑1 (MQ=255)                                                  ttAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCggg                                                                   <  2:322068/140‑1 (MQ=255)                                                  ttAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCggg                                                                   <  2:595449/140‑1 (MQ=255)                                                       aTTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTc                                                              <  1:535997/140‑1 (MQ=255)                                                         tttCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCa                                                            <  2:66522/140‑1 (MQ=255)                                                           tCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCgg                                                                    <  1:327197/130‑1 (MQ=255)                                                           tCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCgg                                                                    >  2:327197/1‑130 (MQ=255)                                                           tCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGt                                                          <  1:775780/140‑1 (MQ=255)                                                                   tCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGAt                                                       <  1:835245/135‑1 (MQ=255)                                                                   tCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGAt                                                       >  2:835245/1‑135 (MQ=255)                                                                       gATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATg                                              <  1:735831/140‑1 (MQ=255)                                                                       gATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATg                                              <  1:400125/140‑1 (MQ=255)                                                                          atataACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCg                                           >  2:327866/1‑140 (MQ=255)                                                                               aCGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCaa                                      >  1:329769/1‑140 (MQ=255)                                                                                 gTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAg                                    >  1:255584/1‑140 (MQ=255)                                                                                  tCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGa                                   >  1:41046/1‑140 (MQ=255)                                                                                  tCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGa                                   >  1:545653/1‑140 (MQ=255)                                                                                   cGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGAt                                  >  1:753936/1‑140 (MQ=255)                                                                                    ggTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATg                                 >  2:922528/1‑140 (MQ=255)                                                                                     gTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATgg                                >  2:890268/1‑140 (MQ=255)                                                                                     gTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATgg                                >  2:59945/1‑140 (MQ=255)                                                                                     gTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATgg                                <  2:539582/140‑1 (MQ=255)                                                                                          taAACAGACTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTa                           <  1:118612/140‑1 (MQ=255)                                                                                                gACTATTCTCATAGGTAGGCCAAATGTGGATAGCCGGATGAAAAAAAAGACAGCAATAATTTCTGCAGTCGGACTGGCGTTTGCTGCATCCGGGAGTTCCAGTGATTACGTCATGGCGAACCAAGATGGTCTTACGAttt                     >  2:479166/1‑140 (MQ=255)                                                                                                 aCTATTATCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCCACCAAAGATGGCCGTATGAtttt                    >  1:542463/1‑140 (MQ=255)                                                                                                     ttatCATAGGTAGGCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGAcc                >  2:777820/1‑140 (MQ=255)                                                                                                           taggtaggCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGa          >  1:45060/1‑140 (MQ=255)                                                                                                           taggtaggCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGa          >  2:383611/1‑140 (MQ=255)                                                                                                           taggtaggCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGa          >  1:155655/1‑140 (MQ=255)                                                                                                           taggtagaCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGa          >  2:849669/1‑140 (MQ=255)                                                                                                             ggtaggCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGaaa        >  1:294862/1‑140 (MQ=255)                                                                                                               taggCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAAc      >  2:526207/1‑140 (MQ=255)                                                                                                               taggCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAAc      >  2:1076001/1‑140 (MQ=255)                                                                                                                 ggCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGTCGTATGATTTTGACCGATGGAAAACCt    <  2:545653/140‑1 (MQ=255)                                                                                                                 ggCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGTCGTATGATTTTGACCGATGGAAAACCt    <  2:41046/140‑1 (MQ=255)                                                                                                                  gCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTTATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAACCTg   >  2:528582/1‑140 (MQ=255)                                                                                                                  gCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGAc                 <  1:471649/126‑1 (MQ=255)                                                                                                                  gCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGAc                 >  2:471649/1‑126 (MQ=255)                                                                                                                  gCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAACCTg   >  1:945748/1‑140 (MQ=255)                                                                                                                  gCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAACCTg   >  1:488815/1‑140 (MQ=255)                                                                                                                  gCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAACCTg   >  2:731594/1‑140 (MQ=255)                                                                                                                  gCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGAT‑GAAAACCTGa  >  1:213531/1‑140 (MQ=255)                                                                                                                  gCCAAATGAGGATAGCCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGAGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAACCTg   >  2:466918/1‑140 (MQ=255)                                                                                                                                |                                                                                                                               CGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAACCTGA  >  NC_000913/2971139‑2971392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑