Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,996,387 T→C R297R (AGA→AGG)  insD‑4 ← IS2 insertion element protein InsB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,996,3870TC100.0% 229.4 / NA 62R297R (AGA→AGG) insD‑4IS2 insertion element protein InsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/33);  total (29/33)

TAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGC  >  NC_000913/2996253‑2996491
                                                                                                                                      |                                                                                                        
tAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGtt                                                                                                     >  1:646840/1‑140 (MQ=255)
tAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGtt                                                                                                     <  1:530114/140‑1 (MQ=255)
tAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGtt                                                                                                     >  2:216974/1‑140 (MQ=255)
 aGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTa                                                                                                    <  1:203759/140‑1 (MQ=255)
   tACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATc                                                                                                  >  1:685317/1‑140 (MQ=255)
   tACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATc                                                                                                  >  1:362438/1‑140 (MQ=255)
      aTCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACt                                                                                               <  1:637577/140‑1 (MQ=255)
       tCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACtt                                                                                              >  1:508183/1‑140 (MQ=255)
        cAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTa                                                                                             >  1:474622/1‑140 (MQ=255)
        cAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTa                                                                                             >  2:140231/1‑140 (MQ=255)
           gAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTa                                                                                                    >  2:167767/1‑130 (MQ=255)
           gAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTa                                                                                                    <  1:167767/130‑1 (MQ=255)
           gAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAAcc                                                                                          <  2:508183/140‑1 (MQ=255)
           gAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAAcc                                                                                          >  2:1034924/1‑140 (MQ=255)
           gAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAAcc                                                                                          <  2:898790/140‑1 (MQ=255)
             aCCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACt                                                                                               >  2:688354/1‑133 (MQ=255)
             aCCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACt                                                                                               <  1:688354/133‑1 (MQ=255)
             aCCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCa                                                                                        >  2:170596/1‑140 (MQ=255)
             aCCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCa                                                                                        >  2:134023/1‑140 (MQ=255)
                ttATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTa                                                                                     >  1:524566/1‑140 (MQ=255)
                 tataAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTa                                                                                             >  1:319912/1‑131 (MQ=255)
                 tataAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTa                                                                                             <  2:319912/131‑1 (MQ=255)
                 tataAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTAc                                                                                    >  2:149098/1‑140 (MQ=255)
                 tataAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTAc                                                                                    >  2:602908/1‑140 (MQ=255)
                  ataAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTa                                                                                                    >  1:894767/1‑123 (MQ=255)
                  ataAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTa                                                                                                    <  2:894767/123‑1 (MQ=255)
                  ataAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACa                                                                                   <  1:608647/140‑1 (MQ=255)
                        aaaCCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCg                                                                             <  2:362438/140‑1 (MQ=255)
                        aaaCCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCg                                                                             <  2:1035825/140‑1 (MQ=255)
                         aaCCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGc                                                                            <  1:149098/140‑1 (MQ=255)
                         aaCCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGc                                                                            >  1:671985/1‑140 (MQ=255)
                           cccGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTg                                                                          <  2:100500/140‑1 (MQ=255)
                            ccGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGc                                                                         <  1:519726/140‑1 (MQ=255)
                              gcgTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                       <  1:170596/140‑1 (MQ=255)
                                              tctcGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgc                                                       <  1:512126/140‑1 (MQ=255)
                                              tctcGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgc                                                       <  2:646840/140‑1 (MQ=255)
                                               ctcGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                      <  1:134023/140‑1 (MQ=255)
                                               ctcGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                      <  2:524566/140‑1 (MQ=255)
                                                              aaCCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                       <  2:685317/140‑1 (MQ=255)
                                                              aaCCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                       <  1:462972/140‑1 (MQ=255)
                                                              aaCCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                       <  1:259414/140‑1 (MQ=255)
                                                                    tGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTAt                                 >  2:300207/1‑140 (MQ=255)
                                                                         aaaCAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGa                            <  2:474622/140‑1 (MQ=255)
                                                                          aaCAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGAt                           >  1:851682/1‑140 (MQ=255)
                                                                           aCAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATg                          <  2:671985/140‑1 (MQ=255)
                                                                              gATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCa                       <  1:216073/140‑1 (MQ=255)
                                                                              gATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCa                       <  2:310068/140‑1 (MQ=255)
                                                                                tCAATGGAAGTAAATTCCAGTTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGCGCGATGACCCACCGCACTATGCGGATGCCAtt                     >  1:277692/1‑140 (MQ=17)
                                                                                tCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                     <  2:391503/140‑1 (MQ=255)
                                                                                tCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                     >  1:133822/1‑140 (MQ=255)
                                                                                tCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                     >  1:832290/1‑140 (MQ=255)
                                                                                  aaTGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCg                   >  1:610186/1‑140 (MQ=255)
                                                                                  aaTGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCg                   >  2:678367/1‑140 (MQ=255)
                                                                                  aaTGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCg                   >  2:809345/1‑140 (MQ=255)
                                                                                         gTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAAt            >  2:449302/1‑140 (MQ=255)
                                                                                         gTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAAt            >  2:88264/1‑140 (MQ=255)
                                                                                         gTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAAt            >  2:893790/1‑140 (MQ=255)
                                                                                            aaTTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCt         >  1:874944/1‑140 (MQ=255)
                                                                                                ccAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa     <  1:678367/140‑1 (MQ=38)
                                                                                                ccAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa     <  1:300207/140‑1 (MQ=38)
                                                                                                ccAATTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa     <  1:893790/140‑1 (MQ=38)
                                                                                                   aTTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGc  <  2:610186/140‑1 (MQ=25)
                                                                                                   aTTGTTTATTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGc  <  1:944130/140‑1 (MQ=25)
                                                                                                                                      |                                                                                                        
TAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGC  >  NC_000913/2996253‑2996491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑