| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,008,069 | T→C | V187A (GTA→GCA) | ygeX → | 2,3‑diaminopropionate ammonia‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,008,069 | 0 | T | C | 98.3% | 211.5 / ‑5.8 | 58 | V187A (GTA→GCA) | ygeX | 2,3‑diaminopropionate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (33/24); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGACGCACGTTCTGCTGCAA > NC_000913/3007936‑3008199 | ccGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGa < 2:143375/140‑1 (MQ=255) gAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGaca < 2:872767/140‑1 (MQ=255) aaGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACg > 2:424005/1‑140 (MQ=255) aGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACgg < 1:218456/140‑1 (MQ=255) cTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGaa > 1:795229/1‑140 (MQ=255) ggAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATggg > 2:668765/1‑134 (MQ=255) ggAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATggg < 1:668765/134‑1 (MQ=255) cgTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCa > 1:538671/1‑140 (MQ=255) tGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCaaaa > 1:922620/1‑140 (MQ=255) aCGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATc < 1:417018/140‑1 (MQ=255) aCGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATc < 2:795229/140‑1 (MQ=255) aCGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATc < 1:657187/140‑1 (MQ=255) aCGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATc > 2:914136/1‑140 (MQ=255) aCGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATc < 2:724417/140‑1 (MQ=255) aCGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATc < 2:922620/140‑1 (MQ=255) cGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATcc < 2:196295/140‑1 (MQ=255) cATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCaa < 2:164078/140‑1 (MQ=255) cATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCaa > 2:1008089/1‑140 (MQ=255) aTTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAAc < 1:424005/140‑1 (MQ=255) ttCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAAcc > 1:590926/1‑140 (MQ=255) cTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTg < 1:434803/140‑1 (MQ=255) gAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGa < 2:538671/140‑1 (MQ=255) gAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGa < 1:1008089/140‑1 (MQ=255) cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGa > 1:801455/1‑134 (MQ=255) cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGa < 2:801455/134‑1 (MQ=255) cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGc < 2:66120/140‑1 (MQ=255) cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGc > 2:860875/1‑140 (MQ=255) cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGc > 2:328235/1‑140 (MQ=255) gTGCCGAGTGCATCGGCACGGATATGAACTATGACGATACCGGTCGCCTGACCATGCGCCACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACCCGGCATGGGCAGGGTACACGCAAATCCACACCTGGCTCTTGCaa > 2:546010/1‑140 (MQ=255) tGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAAc > 1:268990/1‑126 (MQ=255) tGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAAc < 2:268990/126‑1 (MQ=255) ccGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGc > 1:813611/1‑140 (MQ=255) aTCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAc > 2:131542/1‑140 (MQ=255) cACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTgg < 1:914136/140‑1 (MQ=255) tGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGaa > 1:910087/1‑140 (MQ=255) tATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAcc < 1:1041537/123‑1 (MQ=255) tATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAcc > 2:1041537/1‑123 (MQ=255) tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCg > 2:965487/1‑140 (MQ=255) aCGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAg > 1:727664/1‑140 (MQ=255) aCGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAg > 2:502506/1‑140 (MQ=255) aCGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAg > 2:532542/1‑140 (MQ=255) gATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCa < 1:502506/140‑1 (MQ=255) aTACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCaa > 2:209894/1‑140 (MQ=255) gTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCg > 1:786787/1‑140 (MQ=255) ttCGCCTGACCATGCAACACGCGCAGCAGCACGGGTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGt > 2:976213/1‑140 (MQ=255) ttCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGt > 2:105221/1‑140 (MQ=255) ttCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGt > 2:337497/1‑140 (MQ=255) ttCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGt > 2:179537/1‑140 (MQ=255) gCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa > 1:554883/1‑140 (MQ=255) gCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa < 1:167853/140‑1 (MQ=255) cTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa > 1:219673/1‑138 (MQ=255) cTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa < 2:219673/138‑1 (MQ=255) gCAACACGCGCAGCAGCACGGCTGGGCAGAGGCACAGGACACGGCATGGGACGGCTACACCAAAATCCCAACCTGGCTCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTaa > 2:371195/1‑140 (MQ=255) aaCACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAAcc > 1:85131/1‑140 (MQ=255) aaCACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAAcc > 1:360042/1‑140 (MQ=255) aaCACGCGCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGAACATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAAcc > 2:525615/1‑140 (MQ=255) cgcgCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGa > 1:880564/1‑140 (MQ=255) cgcgCAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGa > 2:391213/1‑140 (MQ=255) gcgcAGCAGCACGGCTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGAc < 2:590926/140‑1 (MQ=255) cTGGGAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGACGCACGTTCtgctgc > 1:1066876/1‑140 (MQ=255) gggAAGTGGCACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGACGCACGTTCTGCTGCaa > 2:57425/1‑140 (MQ=255) | CCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGACGCACGTTCTGCTGCAA > NC_000913/3007936‑3008199 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |