Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,052,204	T→C	E38E (GAA→GAG)	ubiI ←	2‑octaprenylphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,052,204	0	T	C	100.0%	129.0 / NA	36	E38E (GAA→GAG)	ubiI	2‑octaprenylphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/18);  total (18/18)

ATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTATTC  >  NC_000913/3052076‑3052326                                                                                                                                 |                                                                                                                           aTACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTAcgcg                                                                                                                 <  2:658844/140‑1 (MQ=255) aTACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTAcgcg                                                                                                                 <  2:642261/140‑1 (MQ=255) aTACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTAcgcg                                                                                                                 <  1:876537/140‑1 (MQ=255) aTACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTAcgcg                                                                                                                 <  1:758629/140‑1 (MQ=255) aTACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTAcgcg                                                                                                                 <  1:436727/140‑1 (MQ=255)  tACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACgcgc                                                                                                                <  1:953221/140‑1 (MQ=255)     cGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCgctgc                                                                                                             >  1:170250/1‑140 (MQ=255)     cGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCgctgc                                                                                                             <  2:531007/140‑1 (MQ=255)     cGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCgctgc                                                                                                             >  1:171819/1‑140 (MQ=255)       tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTAcgcg                                                                                                                 >  1:597085/1‑134 (MQ=255)       tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTAcgcg                                                                                                                 <  2:597085/134‑1 (MQ=255)       tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACgcgc                                                                                                                >  1:484031/1‑135 (MQ=255)       tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACgcgc                                                                                                                <  2:484031/135‑1 (MQ=255)       tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTc                                                                                                           >  1:217174/1‑140 (MQ=255)       tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTc                                                                                                           >  1:763753/1‑140 (MQ=255)           aaCAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGt                                                                                                       <  1:853969/140‑1 (MQ=255)               gCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTAcgcg                                                                                                                 >  2:559679/1‑126 (MQ=255)               gCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTAcgcg                                                                                                                 <  1:559679/126‑1 (MQ=255)                         cGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTaa                                                                                         >  1:764496/1‑140 (MQ=255)                              gagaATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGc                                                                                    >  1:90609/1‑140 (MQ=255)                               agaATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCt                                                                                   >  1:694430/1‑140 (MQ=255)                                   tGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTcccc                                                                               >  1:385071/1‑140 (MQ=255)                                     tCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTc                                                                                  >  1:405765/1‑135 (MQ=255)                                     tCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTc                                                                                  <  2:405765/135‑1 (MQ=255)                                         gCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAg                                                                         <  1:323049/140‑1 (MQ=255)                                             gACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCcac                                                                     <  1:996225/140‑1 (MQ=255)                                              aCGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCcaca                                                                    >  1:320800/1‑140 (MQ=255)                                                 ccAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGc                                                                 <  1:73231/140‑1 (MQ=255)                                                      gcGGGTGAGTATTTTTTCGCTGGCGGCATTGATACCCGAAACGCGCAGTTGTGGTGGTCCATGCGCCCCCAGGGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCg                                                            <  2:170250/139‑1 (MQ=255)                                                      aCGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCg                                                            <  2:171819/140‑1 (MQ=255)                                                        gggTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGcc                                                          >  1:1036972/1‑140 (MQ=255)                                                          gTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAg                                                        >  1:735568/1‑140 (MQ=255)                                                                        gCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATgccgc                                          <  2:385071/140‑1 (MQ=255)                                                                               gcATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCcaac                                   <  1:571981/140‑1 (MQ=255)                                                                                     aaaGCCGAACCGCGCGGTTGGGGGGGTGCATGCCCCCCCAGAGGCTCCTGTGCCCGCTGCTCCGGTCGGGCAACGCGTAAGCCGCTCCCCTGAAACCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGc                             <  2:694430/138‑1 (MQ=255)                                                                                     aTAGCCGAAACGCGCAGTTGTGGTGGTGCATCCGCCGCCAGAGGCTCCTGTACGCGCTGCCCCAGTACGGCACCGCGTAAGCCGCCCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGc                             <  2:1037642/140‑1 (MQ=255)                                                                                      tAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCt                            <  2:320800/140‑1 (MQ=255)                                                                                      tAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCt                            >  2:17801/1‑140 (MQ=255)                                                                                          cGAAACGCGCAGTTGGGGTGGTGTATCCCCCGCCAGAGGCCCCTGTACGCGCTCCCCCAGTCCGGCACCGCGTAAGCCGCCCCCCTGAAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACAt                        <  2:17425/140‑1 (MQ=255)                                                                                                 cgcAGTTGTGGTGGTGCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCCCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACt                 <  2:217174/140‑1 (MQ=255)                                                                                                             ggtgCATCCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTCCGGCACCGCGTAAGCCGCCCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTa     <  2:735568/140‑1 (MQ=255)                                                                                                             ggtgCATCCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTa     <  2:90609/140‑1 (MQ=255)                                                                                                              gtgCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt    >  2:49216/1‑140 (MQ=255)                                                                                                                gCATTCGCCGCCAGAGGCTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTATTc  >  2:776159/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                           ATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTATTC  >  NC_000913/3052076‑3052326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑