Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,159,541	T→C	M536V (ATG→GTG)	ygiQ ←	radical SAM superfamily protein YgiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,159,541	0	T	C	100.0%	287.8 / NA	78	M536V (ATG→GTG)	ygiQ	radical SAM superfamily protein YgiQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/34);  total (44/34)

ATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCATAACGCACACCAGA  >  NC_000913/3159404‑3159667                                                                                                                                          |                                                                                                                               aTTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTtcacca                                                                                                                              <  1:772844/140‑1 (MQ=255)          atCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATa                                                                                                                     <  2:599931/140‑1 (MQ=255)           tCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATaa                                                                                                                    >  2:413090/1‑140 (MQ=255)           tCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATaa                                                                                                                    >  1:442355/1‑140 (MQ=255)            cTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAATTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAAc                                                                                                                   <  2:982631/140‑1 (MQ=255)               catcaCGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGc                                                                                                                >  2:546956/1‑140 (MQ=255)               catcaCGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACATGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGc                                                                                                                >  1:14184/1‑140 (MQ=255)                atcaCGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGcc                                                                                                               >  1:783221/1‑140 (MQ=255)                atcaCGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGcc                                                                                                               >  1:597768/1‑140 (MQ=255)                atcaCGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGcc                                                                                                               <  1:546956/140‑1 (MQ=255)                atcaCGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGcc                                                                                                               <  2:43111/140‑1 (MQ=255)                 tcaCGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGccc                                                                                                              >  2:560165/1‑140 (MQ=255)                    cgcgTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGc                                                                                                                >  2:336653/1‑135 (MQ=255)                    cgcgTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGc                                                                                                                <  1:336653/135‑1 (MQ=255)                    cgcgTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGc                                                                                                                <  1:856689/135‑1 (MQ=255)                    cgcgTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGc                                                                                                                >  2:856689/1‑135 (MQ=255)                    cgcgTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCcttc                                                                                                           >  1:932579/1‑140 (MQ=255)                     gcgTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCcttct                                                                                                          <  1:817932/140‑1 (MQ=255)                          ccGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGt                                                                                                     <  1:535608/140‑1 (MQ=255)                          ccGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGt                                                                                                     <  2:675304/140‑1 (MQ=255)                          ccGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGt                                                                                                     <  1:559590/140‑1 (MQ=255)                               gTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGtt                                                                                                >  1:825657/1‑140 (MQ=255)                                  cgcgGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCg                                                                                             >  2:817031/1‑140 (MQ=255)                                  cgcgGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCg                                                                                             >  2:138377/1‑140 (MQ=255)                                  cgcgGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCg                                                                                             <  1:138377/140‑1 (MQ=255)                                     ggAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCgggg                                                                                          <  2:932579/140‑1 (MQ=255)                                     ggAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCgggg                                                                                          <  2:118979/140‑1 (MQ=255)                                         aTGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAAt                                                                                      <  1:560165/140‑1 (MQ=255)                                                aacagaccAGAAACTGCTCTTTACCTGCATGTTTTGAGTAAGTATCGACCAGCTCTTAAAAGCGGTCATAGCCGCCACCCCCCGGATTCTCCAGCTACGAAAGCGCCCCTTCTTGGGTCTTTTCCGGGAAAATCTTCAGa                                                                               <  2:170564/133‑1 (MQ=255)                                                 ggggATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGAt                                                                              <  2:452626/140‑1 (MQ=255)                                                 ggggATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGAt                                                                              >  1:1083602/1‑140 (MQ=255)                                                    gATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAc                                                                           >  2:225422/1‑140 (MQ=255)                                                       cAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAAccgc                                                                        >  1:375119/1‑140 (MQ=255)                                                         gATACTGCTCTTTCCCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCCCCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGAAATCTTCAGATAAccgccg                                                                      <  1:44962/140‑1 (MQ=255)                                                           tcttGCTTTTTCCCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCCCCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATTTTCAGATAACCGCCGAc                                                                    <  2:226355/137‑1 (MQ=255)                                                               gCTTTTTCCCTGCCTGTTTGGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTAAGATAACCGCCGACGTGa                                                                <  2:442355/140‑1 (MQ=255)                                                               gCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGa                                                                >  1:194632/1‑140 (MQ=255)                                                               gCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGa                                                                >  2:707284/1‑140 (MQ=255)                                                                ctctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGAt                                                               >  2:306859/1‑140 (MQ=255)                                                                ctctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGAt                                                               >  2:604118/1‑140 (MQ=255)                                                                ctctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGAt                                                               >  2:9777/1‑140 (MQ=255)                                                                ctctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGAt                                                               >  2:840244/1‑140 (MQ=255)                                                                ctctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACCTgtc                                                               >  1:170564/1‑138 (MQ=255)                                                                 tctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATg                                                              >  2:770114/1‑140 (MQ=255)                                                                  ttttACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATgg                                                             <  1:407964/139‑1 (MQ=255)                                                                  ctTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATgg                                                             <  2:375119/140‑1 (MQ=255)                                                                    ttACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGt                                                           >  1:294523/1‑140 (MQ=255)                                                                    ttACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGt                                                           >  1:913326/1‑140 (MQ=255)                                                                        ctgcctgTTTTGAGTAAGTATCGAACAGCTTTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGcc                                                       <  1:865366/140‑1 (MQ=255)                                                                        ctgcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGcc                                                       <  1:615177/140‑1 (MQ=255)                                                                        ctgcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGcc                                                       >  1:838098/1‑140 (MQ=255)                                                                        ctgcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGcc                                                       >  2:793305/1‑140 (MQ=255)                                                                          gcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCCCCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAg                                                     <  2:584718/140‑1 (MQ=255)                                                                          gcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAg                                                     <  2:14184/140‑1 (MQ=255)                                                                          gcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAg                                                     >  1:750417/1‑140 (MQ=255)                                                                          gcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAg                                                     <  1:604118/140‑1 (MQ=255)                                                                          gcctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAg                                                     <  1:817031/140‑1 (MQ=255)                                                                           cctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAg                                                     <  2:421727/139‑1 (MQ=255)                                                                           cctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAg                                                     >  1:421727/1‑139 (MQ=255)                                                                            ctgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGtt                                                   <  2:294523/140‑1 (MQ=255)                                                                             tgTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTc                                                  <  1:225422/140‑1 (MQ=255)                                                                               ttttGAGTAAGTATGGAACATCTCTTAAAGGCGGTAATAGCTCCCCATCCCCGGCTTCACCTCCTTCGATACCGGCCCTTCTTCGGTATTTTCCGGGGCATTCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCtt                                                <  2:825657/140‑1 (MQ=255)                                                                               ttttGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCtt                                                >  2:1054321/1‑140 (MQ=255)                                                                               ttttGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCtt                                                >  1:399696/1‑140 (MQ=255)                                                                               ttttGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCtt                                                >  1:720033/1‑140 (MQ=255)                                                                                     gtaagtaTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGatat                                          >  1:279960/1‑140 (MQ=255)                                                                                         gtaTCGTACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAgcg                                      >  2:384898/1‑140 (MQ=255)                                                                                         gtaTCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAgcg                                      <  2:913326/140‑1 (MQ=255)                                                                                             cGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGa                                  <  2:720033/140‑1 (MQ=255)                                                                                                aCAGCTCTTTAAAGCGGTCATAGCTGCCCATACCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGAtct                               >  1:743576/1‑140 (MQ=255)                                                                                                    ctctTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGGTAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTa                           >  2:364781/1‑140 (MQ=255)                                                                                                     ttttaaaaGGGGTCATAGCCGCCCTTCCCCGGCTTCACCATCTTCGAAAAGGCCCTTTCTTGGGTATGTTCCGGGGAAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTAc                          <  2:1083602/135‑1 (MQ=255)                                                                                                       tttAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACgg                        <  1:840244/140‑1 (MQ=255)                                                                                                         tAAAGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCt                      <  1:364781/140‑1 (MQ=255)                                                                                                          aaaGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTa                     <  2:399696/140‑1 (MQ=255)                                                                                                          aaaGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTa                     >  2:756420/1‑140 (MQ=255)                                                                                                          aaaGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTa                     <  1:384898/140‑1 (MQ=255)                                                                                                            aGCGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATg                   <  2:838098/140‑1 (MQ=255)                                                                                                              cGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGAtcttc                             >  2:979542/1‑128 (MQ=255)                                                                                                              cGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGAtcttc                             <  1:979542/128‑1 (MQ=255)                                                                                                              cGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTc                 >  1:738018/1‑140 (MQ=255)                                                                                                              cGGTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTc                 >  1:40918/1‑140 (MQ=255)                                                                                                               ggTCATAGCTGCCCATGCCCGGCTTCCCCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCa                <  2:912071/140‑1 (MQ=255)                                                                                                               ggTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCa                <  2:40918/140‑1 (MQ=255)                                                                                                               ggTCATAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCa                >  1:315574/1‑140 (MQ=255)                                                                                                                    tAGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCATAACg           <  2:783221/140‑1 (MQ=255)                                                                                                                    tAGCTGCCCACGCCCGGCTTCACCTCCGTAGAAAACGGCCTTTTTCGGGTATTTTCCGGGAAATTCTTCAGACAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCATAACg           <  2:682228/140‑1 (MQ=255)                                                                                                                     aGCTGCCCATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCATAACGc          <  1:9777/140‑1 (MQ=255)                                                                                                                          cccATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCATAACGCACAcc     >  2:1096662/1‑140 (MQ=255)                                                                                                                            cATGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGCCAATCTTCAGATACCCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCATAACGCACACCag   <  2:597768/140‑1 (MQ=255)                                                                                                                             aTGCCCGGCTTCACCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCATAACGCACACCaga  >  1:1091989/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                               ATTCACCATATCTTCATCACGCGTACCGGGGTGCGCGGAGATGAAATAGGGGATCAGATACTGCTCTTTACCTGCCTGTTTTGAGTAAGTATCGAACAGCTCTTTAAAGCGGTCATAGCTGCCCATGCCCGGCTTCATCATCTTCGATAACGGCCCTTCTTCGGTATGTTCCGGGGCAATCTTCAGATAACCGCCGACGTGATGGGTCGCCAGTTCTTTGATATAGCGCGGATCTTCTACGGCTATGTCATAACGCACACCAGA  >  NC_000913/3159404‑3159667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑