Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,331,475	T→C	T94A (ACG→GCG)	obgE ←	GTPase ObgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,331,475	0	T	C	100.0%	178.6 / NA	49	T94A (ACG→GCG)	obgE	GTPase ObgE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/22);  total (27/22)

TAACGGACGATTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCT  >  NC_000913/3331352‑3331611                                                                                                                            |                                                                                                                                         tAACGGACGATTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTa                                                                                                                          <  1:395024/140‑1 (MQ=255)   aCGGACGATTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAAt                                                                                                                        >  2:970296/1‑140 (MQ=255)   aCGGACGATTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAAt                                                                                                                        >  1:1067566/1‑140 (MQ=255)   aCGGACGATTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAAt                                                                                                                        >  1:637996/1‑140 (MQ=255)                aaCGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTa                                                                                                           <  1:970296/140‑1 (MQ=255)                     gTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCa                                                                                                                   >  2:577664/1‑127 (MQ=255)                     gTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCa                                                                                                                   <  1:577664/127‑1 (MQ=255)                     gTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACcgcg                                                                                                      <  2:910897/140‑1 (MQ=255)                     gTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACcgcg                                                                                                      <  1:919015/140‑1 (MQ=255)                     gTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACcgcg                                                                                                      <  2:903198/140‑1 (MQ=255)                     gTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACcgcg                                                                                                      >  1:48411/1‑140 (MQ=255)                     gTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACcgcg                                                                                                      <  2:946347/140‑1 (MQ=255)                       aTTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCTATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCt                                                                                                    <  1:740791/140‑1 (MQ=255)                             cAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCgg                                                                                              >  2:692421/1‑140 (MQ=255)                               gACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTa                                                                                                           >  1:616339/1‑125 (MQ=255)                               gACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTa                                                                                                           <  2:616339/125‑1 (MQ=255)                               gACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTa                                                                                            <  2:48411/140‑1 (MQ=255)                                aCCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTAc                                                                                           >  1:29618/1‑140 (MQ=255)                                  cGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAg                                                                                         >  1:748380/1‑140 (MQ=255)                                  cGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAg                                                                                         >  2:541986/1‑140 (MQ=255)                                    tGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACcgcg                                                                                                      >  1:236205/1‑125 (MQ=255)                                    tGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACcgcg                                                                                                      <  2:236205/125‑1 (MQ=255)                                    tGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTc                                                                                       >  2:575600/1‑140 (MQ=255)                                     gCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTcg                                                                                      >  2:935835/1‑140 (MQ=255)                                         gccgccCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGc                                                                                  <  1:692421/140‑1 (MQ=255)                                          ccgccCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCt                                                                                 >  1:17192/1‑140 (MQ=255)                                             cccTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTgc                                                                              <  1:128975/140‑1 (MQ=255)                                               cTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTgcgc                                                                            >  1:314372/1‑140 (MQ=255)                                                    cAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTc                                                                       <  2:748380/140‑1 (MQ=255)                                                         agcagACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGAcc                                                                  <  1:1090464/140‑1 (MQ=255)                                                            agACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACcgcg                                                               <  1:575600/140‑1 (MQ=255)                                                             gACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCgcgc                                                              <  2:29618/140‑1 (MQ=255)                                                                        tgtTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGaa                                                   >  1:974358/1‑140 (MQ=255)                                                                                   atCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCaa                                        >  1:722553/1‑140 (MQ=255)                                                                                          aTGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATa                                 <  2:637996/140‑1 (MQ=255)                                                                                          aTGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATa                                 <  2:1067566/140‑1 (MQ=255)                                                                                           tGGTTTCACCAGTACCCTGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATaa                                >  1:1086923/1‑140 (MQ=255)                                                                                                            tGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATa                                 <  1:931986/122‑1 (MQ=255)                                                                                                            tGGTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATa                                 >  2:931986/1‑122 (MQ=255)                                                                                                             ggTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGGGCTCTGCACGGAAAGATTTTTCAAAACGAAAATAGCTAAACGTGTTTAgg              >  2:1097585/1‑140 (MQ=255)                                                                                                              gTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCATGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGt             >  1:916198/1‑140 (MQ=255)                                                                                                              gTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGt             >  2:834147/1‑140 (MQ=255)                                                                                                              gTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGt             >  1:784835/1‑140 (MQ=255)                                                                                                              gTCGATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGt             >  1:365540/1‑140 (MQ=255)                                                                                                                 gATTACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTtct          >  2:364907/1‑140 (MQ=255)                                                                                                                   ttACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCtcg        >  1:91090/1‑140 (MQ=255)                                                                                                                   ttACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCtcg        >  1:813267/1‑140 (MQ=255)                                                                                                                    tACACGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCtcgt       <  2:649240/140‑1 (MQ=255)                                                                                                                       acGCGCACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCgg    <  2:91090/140‑1 (MQ=255)                                                                                                                         gcgcACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCt  <  2:784835/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                         TAACGGACGATTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGACGCTGACCGTGTTTGGTCATATCGCCCATGGTTTCACCAGTACCCTGGTCGATTACACGCGTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCT  >  NC_000913/3331352‑3331611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑