Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,353,702	T→C	T117A (ACC→GCC)	yhcC ←	radical SAM family oxidoreductase YhcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,353,702	0	T	C	100.0%	256.0 / NA	70	T117A (ACC→GCC)	yhcC	radical SAM family oxidoreductase YhcC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/35);  total (35/35)

TCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGTACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGTTTAGCGCGGTTAACTAA  >  NC_000913/3353566‑3353834                                                                                                                                         |                                                                                                                                     tCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCAcc                                                                                                                                   <  1:126451/140‑1 (MQ=255)    tGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCcac                                                                                                                                >  1:979763/1‑140 (MQ=255)     ggCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCcaca                                                                                                                               >  2:962847/1‑140 (MQ=255)          cgGTTGATGCGATGCAGTGTTTTATCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACaa                                                                                                                          <  1:109052/140‑1 (MQ=255)                     atgcAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAatat                                                                                                               >  2:389492/1‑140 (MQ=255)                     atgcAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAatat                                                                                                               >  1:791048/1‑140 (MQ=255)                     atgcAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAatat                                                                                                               >  1:355805/1‑140 (MQ=255)                       gcAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGTACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTg                                                                                                             >  2:158422/1‑140 (MQ=255)                           tgtTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCt                                                                                                         <  1:255367/140‑1 (MQ=255)                                tGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTc                                                                                                    <  1:565238/140‑1 (MQ=255)                                tGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTc                                                                                                    >  2:156984/1‑140 (MQ=255)                                tGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTc                                                                                                    <  1:925366/140‑1 (MQ=255)                                tGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTc                                                                                                    >  1:199305/1‑140 (MQ=255)                                    gTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCg                                                                                                <  1:1070586/140‑1 (MQ=255)                                     tGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCg                                                                                                <  2:500969/139‑1 (MQ=255)                                     tGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCg                                                                                                >  1:500969/1‑139 (MQ=255)                                     tGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGc                                                                                               <  2:908830/140‑1 (MQ=255)                                     tGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGc                                                                                               >  1:426524/1‑140 (MQ=255)                                      gggCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGcc                                                                                              <  1:728594/140‑1 (MQ=255)                                      gggCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGcc                                                                                              <  2:878636/140‑1 (MQ=255)                                       ggCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCAcc                                                                                                 <  1:541980/136‑1 (MQ=255)                                       ggCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCAcc                                                                                                 >  2:541980/1‑136 (MQ=255)                                       ggCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCAcc                                                                                                 >  2:815871/1‑136 (MQ=255)                                       ggCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCAcc                                                                                                 <  1:815871/136‑1 (MQ=255)                                          ggTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCctgct                                                                                          <  1:763530/140‑1 (MQ=255)                                             ttGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGAt                                                                                       <  2:37222/140‑1 (MQ=255)                                                tAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACa                                                                                    >  1:661502/1‑140 (MQ=255)                                                   ccccAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAg                                                                                 >  1:598098/1‑140 (MQ=255)                                                        gCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGTACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgc                                                                            <  1:158422/140‑1 (MQ=255)                                                        gCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgc                                                                            >  1:1159/1‑140 (MQ=255)                                                          tCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgcag                                                                          <  2:661502/140‑1 (MQ=255)                                                                   acTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAAc                                                                 <  1:59800/140‑1 (MQ=255)                                                                          aaCCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCa                                                          <  2:598098/140‑1 (MQ=255)                                                                               tGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAAc                                                                 <  1:330808/128‑1 (MQ=255)                                                                               tGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAAc                                                                 >  2:330808/1‑128 (MQ=255)                                                                               tGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGt                                                     <  2:426524/140‑1 (MQ=255)                                                                                ggTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTg                                                    <  2:199305/140‑1 (MQ=255)                                                                                 gTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGATAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGc                                                   <  1:962847/140‑1 (MQ=255)                                                                                 gTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGc                                                   >  2:644533/1‑140 (MQ=255)                                                                                      tataTTCGCAAAGCAGATTCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGta                                              >  1:382054/1‑140 (MQ=255)                                                                                      tataTTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGta                                              >  2:39606/1‑140 (MQ=255)                                                                                      tataTTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGta                                              >  2:706359/1‑140 (MQ=255)                                                                                       atatTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGtat                                             >  2:384451/1‑140 (MQ=255)                                                                                       atatTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGtat                                             >  1:82639/1‑140 (MQ=255)                                                                                        tatTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGtata                                            <  1:389492/140‑1 (MQ=255)                                                                                        tatTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGtata                                            <  1:39606/140‑1 (MQ=255)                                                                                        tatTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGtata                                            <  2:535114/140‑1 (MQ=255)                                                                                           tCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGc                                         >  2:800767/1‑140 (MQ=255)                                                                                            cGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGcc                                        >  1:426327/1‑140 (MQ=255)                                                                                              cAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTg                                      >  1:255688/1‑140 (MQ=255)                                                                                                 aGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGaaa                                   >  2:139878/1‑140 (MQ=255)                                                                                                 aGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGaaa                                   <  2:979763/140‑1 (MQ=255)                                                                                                       tCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGc                             <  2:1159/140‑1 (MQ=255)                                                                                                           gCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGa                         <  1:800767/140‑1 (MQ=255)                                                                                                            cACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGAt                        <  1:156984/140‑1 (MQ=255)                                                                                                            cACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGAt                        <  1:74460/140‑1 (MQ=255)                                                                                                             aCCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATa                       >  1:769177/1‑140 (MQ=255)                                                                                                               cGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGc                     >  2:142811/1‑140 (MQ=255)                                                                                                                 cATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGt                   >  2:116276/1‑140 (MQ=255)                                                                                                                 cATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGt                   >  1:354277/1‑140 (MQ=255)                                                                                                                 cATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGt                   >  2:32965/1‑140 (MQ=255)                                                                                                                 cATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGt                   >  1:307277/1‑140 (MQ=255)                                                                                                                 cATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGt                   >  1:1066346/1‑140 (MQ=255)                                                                                                                  aTCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGtt                  <  1:139878/140‑1 (MQ=255)                                                                                                                      ggCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGTTTAgc              <  2:426327/140‑1 (MQ=255)                                                                                                                       gCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGTTTAgcg             <  1:706359/140‑1 (MQ=255)                                                                                                                           gCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGTTTAGCGCGGt         <  2:355478/140‑1 (MQ=255)                                                                                                                           gCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGTTTAGCGCGGt         >  2:205843/1‑140 (MQ=255)                                                                                                                                  gggcgggcACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGTTTAGCGCGGTTAACTaa  >  2:356352/1‑140 (MQ=255)                                                                                                                                    gcgggCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGTTTAGCGCgg          <  1:64636/130‑1 (MQ=255)                                                                                                                                    gcgggCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGTTTAGCGCgg          >  2:64636/1‑130 (MQ=255)                                                                                                                                         |                                                                                                                                     TCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGTACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACGCCTGAAAGTAGGCCAGATAGCGTTTAGCGCGGTTAACTAA  >  NC_000913/3353566‑3353834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑