Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,398,066	T→C	T105A (ACA→GCA)	yhdE ←	nucleoside triphosphate pyrophosphatase YhdE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,398,066	0	T	C	100.0%	203.7 / NA	55	T105A (ACA→GCA)	yhdE	nucleoside triphosphate pyrophosphatase YhdE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

TATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGC  >  NC_000913/3397930‑3398198                                                                                                                                         |                                                                                                                                     tATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGccat                                                                                                                                   <  2:647557/140‑1 (MQ=255)   tGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGccatca                                                                                                                                 <  1:814696/140‑1 (MQ=255)    gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCAc                                                                                                                                >  2:524600/1‑140 (MQ=255)    gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCAc                                                                                                                                >  2:602111/1‑140 (MQ=255)    gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCAc                                                                                                                                >  2:407969/1‑140 (MQ=255)        cTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGa                                                                                                                            <  2:690121/140‑1 (MQ=255)              tATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTc                                                                                                                      <  2:176000/140‑1 (MQ=255)                  tAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGAc                                                                                                                  >  2:699011/1‑140 (MQ=255)                    aCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGAccc                                                                                                                >  1:518584/1‑140 (MQ=255)                     cGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCg                                                                                                               >  1:24324/1‑140 (MQ=255)                     cGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCg                                                                                                               >  1:884462/1‑140 (MQ=255)                        ttCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATa                                                                                                            >  1:499479/1‑140 (MQ=255)                          cATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAAt                                                                                                          >  1:443750/1‑140 (MQ=255)                          cATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAAt                                                                                                          >  2:946191/1‑140 (MQ=255)                             cGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTg                                                                                                       <  1:602111/140‑1 (MQ=255)                                       aTAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCt                                                                                             >  1:392140/1‑140 (MQ=255)                                            ccGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGcgcc                                                                                        >  1:842920/1‑140 (MQ=255)                                            ccGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGcgcc                                                                                        >  1:509807/1‑140 (MQ=255)                                             cgcgATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGcgccg                                                                                       >  2:748620/1‑140 (MQ=255)                                              gcgATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGcgccgc                                                                                      <  1:407969/140‑1 (MQ=255)                                                         gTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGt                                                                           <  1:748620/140‑1 (MQ=255)                                                              ttAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGGCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTcccgc                                                                      >  2:266833/1‑140 (MQ=255)                                                                aaCGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCgg                                                                    >  1:1049377/1‑140 (MQ=255)                                                                aaCGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCgg                                                                    >  1:122112/1‑140 (MQ=255)                                                                 aCGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGt                                                                   <  2:958064/140‑1 (MQ=255)                                                                    ttCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTtc                                                                <  2:773466/140‑1 (MQ=255)                                                                      cTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTtctc                                                              >  2:193145/1‑140 (MQ=255)                                                                      cTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTtctc                                                              <  1:193145/140‑1 (MQ=255)                                                                        gAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCa                                                            >  2:556612/1‑140 (MQ=255)                                                                         aaaGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAg                                                           <  1:443427/140‑1 (MQ=255)                                                                                caTCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTtct                                                    <  2:1049377/140‑1 (MQ=255)                                                                                caTCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTtct                                                    <  1:990379/140‑1 (MQ=255)                                                                                caTCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTtct                                                    <  2:499479/140‑1 (MQ=255)                                                                                    ggTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGt                                                <  2:392140/140‑1 (MQ=255)                                                                                     gTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGtt                                               >  2:901337/1‑140 (MQ=255)                                                                                     gTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGtt                                               <  1:136889/140‑1 (MQ=255)                                                                                     gTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGtt                                               <  2:1058452/140‑1 (MQ=255)                                                                                      tGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTc                                              <  1:699011/140‑1 (MQ=255)                                                                                        accaccAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAg                                            <  1:556612/140‑1 (MQ=255)                                                                                        accaccAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAg                                            <  2:24324/140‑1 (MQ=255)                                                                                          caccaGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGa                                          <  2:518584/140‑1 (MQ=255)                                                                                           accaGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGAt                                         <  2:433999/140‑1 (MQ=255)                                                                                                    tCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGtt                                               >  2:951903/1‑125 (MQ=255)                                                                                                    tCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGtt                                               <  1:951903/125‑1 (MQ=255)                                                                                                         aaTGTGCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCg                           >  2:978026/1‑140 (MQ=255)                                                                                                             tgCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCAcc                       <  1:508448/140‑1 (MQ=255)                                                                                                             tgCTGGCTGTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCAcc                       <  2:842920/140‑1 (MQ=255)                                                                                                              gctggctgTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCAccc                      >  2:550249/1‑140 (MQ=255)                                                                                                              gctggctgTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCAccc                      >  2:515171/1‑140 (MQ=255)                                                                                                               ctggctgTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCa                     >  2:240167/1‑140 (MQ=255)                                                                                                                tggctgTCGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAg                    <  2:884462/140‑1 (MQ=255)                                                                                                                       cGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGc                   <  2:109981/134‑1 (MQ=255)                                                                                                                       cGGCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGc                   >  1:109981/1‑134 (MQ=255)                                                                                                                         gCCAACGCCACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGgag           <  2:1049834/140‑1 (MQ=255)                                                                                                                                ccACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTcg                                                                         <  2:941187/71‑1 (MQ=255)                                                                                                                                ccACTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTcg                                                                         >  1:941187/1‑71 (MQ=255)                                                                                                                                  aCTGCTGCCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGc  >  1:126283/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGC  >  NC_000913/3397930‑3398198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑