Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,456,220	A→T	I215F (ATC→TTC)	gspC →	Type II secretion system protein GspC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,456,220	0	A	T	100.0%	285.6 / NA	81	I215F (ATC→TTC)	gspC	Type II secretion system protein GspC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (45/36);  total (45/36)

AAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGATCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGAACTT  >  NC_000913/3456083‑3456354                                                                                                                                          |                                                                                                                                       aaaaTGTACTCGCTGATTATATTATCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTc                                                                                                                                      >  2:694608/1‑140 (MQ=255)        aCTCGCTGATTATATTATCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATc                                                                                                                               >  1:488319/1‑140 (MQ=255)        aCTCGCTGATTATATTATCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATc                                                                                                                               <  2:633809/140‑1 (MQ=255)        aCTCGCTGATTATATTATCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATc                                                                                                                               >  2:362955/1‑140 (MQ=255)         cTCGCTGATTATATTATCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCt                                                                                                                              >  1:21335/1‑140 (MQ=255)             cTGATTATATTATCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCg                                                                                                                          <  1:694608/140‑1 (MQ=255)                  tatattatCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGac                                                                                                                     <  2:313302/140‑1 (MQ=255)                   atattatCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGacc                                                                                                                    <  1:991606/140‑1 (MQ=255)                    tattatCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGaccc                                                                                                                   <  2:363642/140‑1 (MQ=255)                     attatCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGaccca                                                                                                                  <  1:704513/140‑1 (MQ=255)                          cGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCtg                                                                                                             >  2:198284/1‑140 (MQ=255)                          cGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCtg                                                                                                             >  1:344476/1‑140 (MQ=255)                             cACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCtgatg                                                                                                          <  1:935290/140‑1 (MQ=255)                                 ccTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGt                                                                                                      <  2:488319/140‑1 (MQ=255)                                    ataCGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTc                                                                                                   >  2:89023/1‑140 (MQ=255)                                     taCGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTca                                                                                                  <  1:734468/140‑1 (MQ=255)                                     taCGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTca                                                                                                  <  1:198284/140‑1 (MQ=255)                                       cGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTcaca                                                                                                <  2:318010/140‑1 (MQ=255)                                       cGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTcaca                                                                                                <  1:362955/140‑1 (MQ=255)                                          gATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAgct                                                                                             >  2:956341/1‑138 (MQ=255)                                          gATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGc                                                                                             >  1:1031318/1‑140 (MQ=255)                                          gATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGc                                                                                             >  2:952315/1‑140 (MQ=255)                                          gATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGc                                                                                             >  1:400394/1‑140 (MQ=255)                                          gATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGc                                                                                             >  2:458065/1‑140 (MQ=255)                                           aTGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCa                                                                                            >  2:391649/1‑140 (MQ=255)                                           aTGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCa                                                                                            >  1:861544/1‑140 (MQ=255)                                            tGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCAt                                                                                           >  2:736356/1‑140 (MQ=255)                                            tGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCAt                                                                                           >  2:1060005/1‑140 (MQ=255)                                               agaACAGATATACGGGCTTCGATTGAACCCTCGTAATGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTaa                                                                                        >  2:552719/1‑140 (MQ=255)                                                 aaCAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGt                                                                                      >  2:1053993/1‑140 (MQ=255)                                                      atatACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCAGAGGCATTAAGTTTACt                                                                                 >  2:988341/1‑140 (MQ=255)                                                      atatACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACt                                                                                 >  1:619580/1‑140 (MQ=255)                                                      atatACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACt                                                                                 <  1:458065/140‑1 (MQ=255)                                                         taCGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCt                                                                              <  2:634359/140‑1 (MQ=255)                                                          aCGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTa                                                                             <  2:1031318/140‑1 (MQ=255)                                                          aCGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTa                                                                             <  1:736356/140‑1 (MQ=255)                                                           cGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTaa                                                                            >  2:1068838/1‑140 (MQ=255)                                                           cGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTaa                                                                            >  2:101227/1‑140 (MQ=255)                                                           cGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTa                                                                             >  2:543799/1‑139 (MQ=255)                                                           cGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTa                                                                             <  1:543799/139‑1 (MQ=255)                                                              gCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTc                                                                         >  2:990306/1‑140 (MQ=255)                                                                ttCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTcaa                                                                       >  1:314885/1‑140 (MQ=255)                                                                 tCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTcaac                                                                      >  1:228531/1‑140 (MQ=255)                                                                   gATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTcaacaa                                                                    <  2:400394/140‑1 (MQ=255)                                                                    aTTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACaaa                                                                   <  1:1060005/140‑1 (MQ=255)                                                                         aCCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCt                                                              <  1:990306/140‑1 (MQ=255)                                                                         aCCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCt                                                              <  1:1068838/140‑1 (MQ=255)                                                                         aCCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCt                                                              <  1:159363/140‑1 (MQ=255)                                                                         aCCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCt                                                              <  2:387664/140‑1 (MQ=255)                                                                           ccTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCa                                                            <  1:952315/140‑1 (MQ=255)                                                                                       cTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATtcg                                                >  2:410764/1‑140 (MQ=255)                                                                                       cTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATtcg                                                >  1:739010/1‑140 (MQ=255)                                                                                       cTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATtcg                                                >  1:697403/1‑140 (MQ=255)                                                                                       cTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATtcg                                                >  2:151347/1‑140 (MQ=255)                                                                                       cTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATtcg                                                >  1:749705/1‑140 (MQ=255)                                                                                          aaTGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATtcgtcg                                             <  2:697403/140‑1 (MQ=255)                                                                                            tGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCa                                           <  2:852869/140‑1 (MQ=255)                                                                                            tGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCa                                           <  2:344476/140‑1 (MQ=255)                                                                                            tGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCa                                           <  1:410764/140‑1 (MQ=255)                                                                                            tGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCa                                           <  2:911599/140‑1 (MQ=255)                                                                                                 ttACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGc                                      >  2:778928/1‑140 (MQ=255)                                                                                                   accaccAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCaa                                          >  2:798731/1‑134 (MQ=255)                                                                                                   accaccAGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCaa                                          <  1:798731/134‑1 (MQ=255)                                                                                                       ccaGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTAccc                                <  1:552719/140‑1 (MQ=255)                                                                                                        caGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTAcccc                               >  2:798715/1‑140 (MQ=255)                                                                                                         aGCTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCg                              <  2:556298/140‑1 (MQ=255)                                                                                                           cTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCt                            >  2:299229/1‑140 (MQ=255)                                                                                                           cTTGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCt                            >  1:465784/1‑140 (MQ=255)                                                                                                             tGCTACAACCAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACACACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTg                          >  2:304922/1‑140 (MQ=255)                                                                                                                     ccAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTAccc                                <  1:224726/126‑1 (MQ=255)                                                                                                                     ccAGGGGATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTAccc                                >  2:224726/1‑126 (MQ=255)                                                                                                                       aGGGTATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGttt                <  2:549469/140‑1 (MQ=255)                                                                                                                        ggggATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTc               >  1:378630/1‑140 (MQ=255)                                                                                                                        ggggATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTc               >  2:326210/1‑140 (MQ=255)                                                                                                                         gggATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTcc              >  2:1055388/1‑140 (MQ=255)                                                                                                                         gggATATCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTcc              <  1:778928/140‑1 (MQ=255)                                                                                                                               tCGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTCCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCggg        >  1:898496/1‑140 (MQ=255)                                                                                                                                cGCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCgggg       >  2:226321/1‑140 (MQ=255)                                                                                                                                 gCCCTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGa      <  1:1055388/140‑1 (MQ=255)                                                                                                                                   ccTCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGAAc    >  1:903603/1‑140 (MQ=255)                                                                                                                                     tCAGGTTCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGAACtt  >  2:952211/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGATGGAGAACAGATATACGGGCTTCGATTGAACCCTCGTAAGGGCCTCAATGCATTTACCACCAGCTTGCTACAACCAGGGGATATCGCCCTCAGGATCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGAACTT  >  NC_000913/3456083‑3456354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑