Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,514,062	T→C	T110A (ACG→GCG)	gph ←	phosphoglycolate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,514,062	0	T	C	100.0%	273.7 / NA	73	T110A (ACG→GCG)	gph	phosphoglycolate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/43);  total (30/43)

GCTGAAGTATTTGGCGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGTCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGC  >  NC_000913/3513925‑3514197                                                                                                                                          |                                                                                                                                        gCTGAAGTATTTGGCGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGccc                                                                                                                                       >  1:1044731/1‑140 (MQ=255) gCTGAAGTATTTGGCCATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGTCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGccc                                                                                                                                       >  1:689994/1‑140 (MQ=255)  cTGAAGTATTTGGCGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGcccc                                                                                                                                      >  2:832120/1‑140 (MQ=255)     aaGTATTTGGCGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCctc                                                                                                                                   >  2:930178/1‑140 (MQ=255)         aTTTGGCGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCg                                                                                                                               <  1:1022854/140‑1 (MQ=255)           ttGGCGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGc                                                                                                                             <  1:703061/140‑1 (MQ=255)             ggCGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCaa                                                                                                                           <  1:513562/140‑1 (MQ=255)               cGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGc                                                                                                                             >  1:843118/1‑136 (MQ=255)               cGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGc                                                                                                                             <  2:843118/136‑1 (MQ=255)               cGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAAcc                                                                                                                         >  1:897208/1‑140 (MQ=255)               cGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAAcc                                                                                                                         >  2:657330/1‑140 (MQ=255)                gATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCt                                                                                                                        <  2:547184/140‑1 (MQ=255)                gATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCt                                                                                                                        <  2:16929/140‑1 (MQ=255)                gATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCt                                                                                                                        <  2:1050350/140‑1 (MQ=255)                gATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCt                                                                                                                        <  1:930178/140‑1 (MQ=255)                 atatCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTc                                                                                                                       <  1:93634/140‑1 (MQ=255)                  tatCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCg                                                                                                                      >  2:1074964/1‑140 (MQ=255)                           cTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCAtagtag                                                                                                             <  2:385236/140‑1 (MQ=255)                            ttCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAAC‑AAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCg                                                                                                           >  1:833306/1‑140 (MQ=255)                              cGAGCCGAGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCg                                                                                                                      >  2:191900/1‑128 (MQ=255)                              cGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGATCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGa                                                                                                          >  2:1022379/1‑140 (MQ=255)                              cGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCg                                                                                                                      <  1:191900/128‑1 (MQ=255)                              cGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGa                                                                                                          >  1:469329/1‑140 (MQ=255)                              cGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGa                                                                                                          >  1:115573/1‑140 (MQ=255)                              cGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGa                                                                                                          >  2:113229/1‑140 (MQ=255)                                  cagcGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGa                                                                                                      <  1:386675/140‑1 (MQ=255)                                  cagcGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGa                                                                                                      <  1:832120/140‑1 (MQ=255)                                  cagcGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGa                                                                                                      <  1:245128/140‑1 (MQ=255)                                  cagcGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGa                                                                                                      <  1:657330/140‑1 (MQ=255)                                   agcGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGaa                                                                                                     <  1:997464/140‑1 (MQ=255)                                    gcggcgCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGTAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAAc                                                                                                    <  1:137813/140‑1 (MQ=255)                                       gcgcGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGt                                                                                                 <  1:1074964/140‑1 (MQ=255)                                          cgacgaACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTa                                                                                              >  1:312238/1‑140 (MQ=255)                                            acgaACGGCGTCGGTTTGTTGGTGACCAGGTCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACg                                                                                            <  2:689994/140‑1 (MQ=255)                                            acgaACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACg                                                                                            <  2:1044731/140‑1 (MQ=255)                                            acgaACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAAC‑AAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGc                                                                                           <  2:833306/140‑1 (MQ=255)                                             cgaACGGCGTCGGTTTGTTGGTGATCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGc                                                                                           <  1:1022379/140‑1 (MQ=255)                                             cgaACGGCGTCGGTTTGTTGGTGACCAGGACTAGCTGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGc                                                                                           <  1:167699/140‑1 (MQ=255)                                              gaACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGACCAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCa                                                                                          <  2:312238/140‑1 (MQ=255)                                              gaACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCa                                                                                          <  2:102770/140‑1 (MQ=255)                                               aaCGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAg                                                                                         >  1:140604/1‑140 (MQ=255)                                                   gCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATa                                                                                     <  1:195231/140‑1 (MQ=255)                                                      tCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGt                                                                                  <  1:415653/140‑1 (MQ=255)                                                                        ggCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGa                                                                >  2:97499/1‑140 (MQ=255)                                                                        ggCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGa                                                                >  1:518188/1‑140 (MQ=255)                                                                                   aGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGtcatc                                                     >  1:479155/1‑140 (MQ=255)                                                                                   aGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGtcatc                                                     >  2:601548/1‑140 (MQ=255)                                                                                   aGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGtcatc                                                     >  1:146696/1‑140 (MQ=255)                                                                                        tttAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACgg                                                <  1:97499/140‑1 (MQ=255)                                                                                        tttAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACgg                                                <  1:365597/140‑1 (MQ=255)                                                                                        tttAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACgg                                                <  1:272936/140‑1 (MQ=255)                                                                                        tttAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACgg                                                <  1:146665/140‑1 (MQ=255)                                                                                              cTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGtt                                          <  2:897208/140‑1 (MQ=255)                                                                                               tGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAAc                                                  >  1:633484/1‑131 (MQ=255)                                                                                               tGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAAc                                                  <  2:633484/131‑1 (MQ=255)                                                                                                 cAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTAc                                       >  1:613468/1‑140 (MQ=255)                                                                                                  aaCGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTAcc                                      <  1:113229/140‑1 (MQ=255)                                                                                                    cgcgCCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCa                                    <  2:130360/140‑1 (MQ=255)                                                                                                      cgcCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCTACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGt                                           <  2:963464/131‑1 (MQ=255)                                                                                                      cgcCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGt                                           >  1:963464/1‑131 (MQ=255)                                                                                                      cgcCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCa                                    <  1:196085/138‑1 (MQ=255)                                                                                                      cgcCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCa                                    >  2:196085/1‑138 (MQ=255)                                                                                                      cgcCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCAtt                                  >  1:191083/1‑140 (MQ=255)                                                                                                      cgcCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCAtt                                  >  1:111956/1‑140 (MQ=255)                                                                                                      cgcCCAACGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCAtt                                  >  1:444179/1‑140 (MQ=255)                                                                                                           aaCGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGtttt                             >  2:557283/1‑140 (MQ=255)                                                                                                            aCGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCa                                    <  2:917555/132‑1 (MQ=255)                                                                                                            aCGTATCGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCa                                    >  1:917555/1‑132 (MQ=255)                                                                                                                  cGGCAACGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGa                      <  1:601548/140‑1 (MQ=255)                                                                                                                        cGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCa                <  2:191083/140‑1 (MQ=255)                                                                                                                        cGTGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCa                >  1:588311/1‑140 (MQ=255)                                                                                                                          tGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGc                         <  1:589286/129‑1 (MQ=255)                                                                                                                          tGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGc                         >  2:589286/1‑129 (MQ=255)                                                                                                                          tGCGGGAACAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACg              >  1:388750/1‑140 (MQ=255)                                                                                                                                  cAAAAACGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGAc      <  2:444179/140‑1 (MQ=255)                                                                                                                                     aaaCGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGAcgcg   >  1:258441/1‑140 (MQ=255)                                                                                                                                      aaCGCCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACgcgc  <  2:258441/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GCTGAAGTATTTGGCGATATCTAAGGCTTCGAGCAGCGGCGCGACGAACGGCGTCGGTTTGTTGGTGACCAGGCCTAGCGGCAGGCCTTTAGCCTGCAACGCGCCCAACGTATCGGCAACGTGCGGGAACAAAAACGTCCCCTCTTCGGCAACCTCGCCATAGTAGCGATCGAACAGTTTACGCAGAATACGTACCTGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGC  >  NC_000913/3513925‑3514197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑