Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,928,600	A→C	F335C (TTT→TGT)	viaA ←	protein ViaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,928,600	0	A	C	96.9%	231.7 / ‑1.5	64	F335C (TTT→TGT)	viaA	protein ViaA
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (31/31);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

CTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCA  >  NC_000913/3928463‑3928735                                                                                                                                          |                                                                                                                                        cTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAg                                                                                                                                       >  1:1035708/1‑140 (MQ=255)    gTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAgccg                                                                                                                                    >  1:200076/1‑140 (MQ=255)         aTGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCAt                                                                                                                               <  2:176845/140‑1 (MQ=255)          tGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCAtt                                                                                                                              >  1:480502/1‑140 (MQ=255)                gtgGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCg                                                                                                                        <  1:225207/140‑1 (MQ=255)                  gggCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGa                                                                                                                      <  2:624863/140‑1 (MQ=255)                  gggCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGa                                                                                                                      <  1:213440/140‑1 (MQ=255)                  gggCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGa                                                                                                                      <  1:404499/140‑1 (MQ=255)                  gggCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGa                                                                                                                      <  1:349301/140‑1 (MQ=255)                  gggCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCACGCAGACCGCTTTCGCACTCTGCTCATTACACCCGCCCATTAAGctcga                                                                                                                      <  2:584076/140‑5 (MQ=255)                   ggCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGaa                                                                                                                     <  2:1057789/140‑1 (MQ=255)                     ccTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGt                                                                                                                   <  1:448246/140‑1 (MQ=255)                      cTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGaa                                                                                                                     >  2:773192/1‑137 (MQ=255)                      cTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGaa                                                                                                                     <  1:773192/137‑1 (MQ=255)                      cTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTa                                                                                                                  <  2:426480/140‑1 (MQ=255)                      cTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTa                                                                                                                  >  1:846676/1‑140 (MQ=255)                         aaaGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGa                                                                                                                      >  1:465525/1‑133 (MQ=255)                         aaaGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATCGAGCCGGa                                                                                                                      <  2:465525/133‑1 (MQ=255)                         aaaGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAACGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATcc                                                                                                               >  1:425230/1‑140 (MQ=255)                            gCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCcaca                                                                                                            <  1:405691/140‑1 (MQ=255)                            gCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCcaca                                                                                                            <  2:518198/140‑1 (MQ=255)                             cTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCcacac                                                                                                           >  2:989824/1‑140 (MQ=255)                             cTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCcacac                                                                                                           <  2:480502/140‑1 (MQ=255)                                       aCGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATaaa                                                                                                 <  1:959558/140‑1 (MQ=255)                                            ctcGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGcc                                                                                            >  1:1056264/1‑140 (MQ=255)                                                gTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCcgcg                                                                                        <  1:436545/140‑1 (MQ=255)                                                   gAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCgg                                                                                     <  1:528639/140‑1 (MQ=255)                                                   gAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCgg                                                                                     >  2:384690/1‑140 (MQ=255)                                                      aaTAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCCGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTg                                                                                  >  2:422047/1‑140 (MQ=255)                                                       aTAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGt                                                                                 <  1:384690/140‑1 (MQ=255)                                                          gCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCa                                                                              <  1:207642/140‑1 (MQ=255)                                                                 ataGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAAt                                                                       <  2:271711/140‑1 (MQ=255)                                                                     cagcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGt                                                                          >  2:616381/1‑133 (MQ=255)                                                                     cagcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGt                                                                          <  1:616381/133‑1 (MQ=255)                                                                     cagcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCttt                                                                   >  1:999817/1‑140 (MQ=255)                                                                     cagcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTA‑CCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTa                                                                  >  1:783756/1‑140 (MQ=255)                                                                     cagcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGtt                                                                                <  2:236937/127‑1 (MQ=255)                                                                     cagcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGtt                                                                                >  1:236937/1‑127 (MQ=255)                                                                        cgcCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATg                                                                <  2:513684/140‑1 (MQ=255)                                                                        cgcCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATg                                                                >  2:341931/1‑140 (MQ=255)                                                                        cgcCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATg                                                                >  1:513684/1‑140 (MQ=255)                                                                          ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATg                                                                <  1:463893/138‑1 (MQ=255)                                                                          ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATg                                                                >  2:463893/1‑138 (MQ=255)                                                                          ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTa                                                              >  1:1079413/1‑140 (MQ=255)                                                                          ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTa                                                              >  2:218394/1‑140 (MQ=255)                                                                          ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTa                                                              >  1:549006/1‑140 (MQ=255)                                                                          ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTa                                                              >  1:800850/1‑140 (MQ=255)                                                                                          gCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGAt                                              <  2:549006/140‑1 (MQ=255)                                                                                                   aTCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGCAATCTTTATGTACCACCGGACGTTCGATCACTTTTTc                                     >  1:149557/1‑140 (MQ=255)                                                                                                      aaGGCCAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACg                                  >  1:196366/1‑140 (MQ=255)                                                                                                          ccAGGCAGAACGCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCAc                              >  2:339189/1‑140 (MQ=255)                                                                                                                ggAACGCTTTCGCACGCTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGGCAATAAACGGCCCGCGCGGCTGTTCATCGTAATGTTTATGTACCACCGGGCGTTCGATCGCTTTTTCACGCCACGACTCa                        <  2:182742/139‑1 (MQ=255)                                                                                                                     gCTTTCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTg                   <  2:1035708/140‑1 (MQ=255)                                                                                                                       tttCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCa                        <  1:286156/133‑1 (MQ=255)                                                                                                                       tttCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCa                        >  2:286156/1‑133 (MQ=255)                                                                                                                       tttCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCa                 <  2:1056264/140‑1 (MQ=255)                                                                                                                        ttCGCACACTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAg                >  1:708566/1‑140 (MQ=255)                                                                                                                            cacaCTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGa            >  1:821064/1‑140 (MQ=255)                                                                                                                            cacaCTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGa            <  1:345321/140‑1 (MQ=255)                                                                                                                              cacTGTTCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATa          <  2:536753/140‑1 (MQ=255)                                                                                                                                cTGTTCATCACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAgg        <  2:86634/140‑1 (MQ=255)                                                                                                                                   ttCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATACACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGa     >  1:194811/1‑140 (MQ=255)                                                                                                                                   ttCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGa     >  1:823960/1‑140 (MQ=255)                                                                                                                                   ttCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGa     >  1:914641/1‑140 (MQ=255)                                                                                                                                   ttCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGa     >  1:808347/1‑140 (MQ=255)                                                                                                                                   ttCATTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGa     >  1:1012596/1‑140 (MQ=255)                                                                                                                                      aTTACAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCa  <  2:149557/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCA  >  NC_000913/3928463‑3928735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑