Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,484	A→G	F113L (TTC→CTC)	ilvY ←	DNA‑binding transcriptional dual regulator IlvY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,484	0	A	G	100.0%	167.1 / NA	45	F113L (TTC→CTC)	ilvY	DNA‑binding transcriptional dual regulator IlvY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/29);  total (16/29)

GGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTT  >  NC_000913/3957363‑3957612                                                                                                                          |                                                                                                                                 ggCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATcggcgg                                                                                                                >  2:792633/1‑140 (MQ=255)    aaGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAg                                                                                                             <  2:790030/140‑1 (MQ=255)     aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGa                                                                                                            <  2:352745/140‑1 (MQ=255)     aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGa                                                                                                            <  2:210923/140‑1 (MQ=255)     aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGa                                                                                                            <  2:664150/140‑1 (MQ=255)     aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGa                                                                                                            <  2:937934/140‑1 (MQ=255)          tCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCt                                                                                                       <  2:408908/140‑1 (MQ=255)                  aCCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATAGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAg                                                                                               <  2:351617/140‑1 (MQ=255)                        aaTCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCa                                                                                         >  2:663432/1‑140 (MQ=255)                         aTCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCAc                                                                                        <  2:453068/140‑1 (MQ=255)                         aTCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCAc                                                                                        >  1:332069/1‑140 (MQ=255)                             ccAGATCCGCTTTACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAg                                                                                    <  2:706726/140‑1 (MQ=255)                             ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAg                                                                                    <  1:257815/140‑1 (MQ=255)                               aGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCa                                                                                  <  2:815085/140‑1 (MQ=255)                                 aTCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCaga                                                                                >  1:395327/1‑140 (MQ=255)                                      cTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGc                                                                                   <  2:899101/132‑1 (MQ=255)                                      cTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGc                                                                                   >  1:899101/1‑132 (MQ=255)                                                 accaccTTTTCCATCGCATCTGCCGCATCACAAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGcc                                                                >  2:641794/1‑140 (MQ=255)                                                         ttCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCg                                                        <  1:190208/140‑1 (MQ=255)                                                         ttCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCg                                                        >  1:450285/1‑140 (MQ=255)                                                         ttCCATCGCATCTGCCGCATCACAAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCg                                                        <  1:641794/140‑1 (MQ=255)                                                              tCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGc                                                   >  1:871800/1‑140 (MQ=255)                                                                gCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGccc                                                 <  1:1036932/140‑1 (MQ=255)                                                                   tCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTg                                              <  1:56380/140‑1 (MQ=255)                                                                           aTCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGa                                      <  2:332069/140‑1 (MQ=255)                                                                            tCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGAt                                     >  2:999139/1‑140 (MQ=255)                                                                              aCCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATgg                                   <  1:663432/140‑1 (MQ=255)                                                                                 agtagtAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGgtgt                                <  1:1049809/140‑1 (MQ=255)                                                                                     gtaAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGgcg                            <  2:275491/140‑1 (MQ=255)                                                                                     gtaAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGgcg                            <  1:280890/140‑1 (MQ=255)                                                                                       aaGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCa                          <  1:792633/140‑1 (MQ=255)                                                                                         gTTTAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAAc                        <  2:1047833/140‑1 (MQ=255)                                                                                            tAATCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAActgc                     >  1:667814/1‑140 (MQ=255)                                                                                             aaTCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAActgct                    <  2:385119/140‑1 (MQ=255)                                                                                             aaTCTCCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAActgct                    >  1:385119/1‑140 (MQ=255)                                                                                             aaTCTCCACCGACGGGGGTTGCGCGCGGAGGCGATCCAGAATCGGCGGCAAATGGCTGGAGGCAGCGGTCGCCGGGCAGAAGAAATGGAATTCGCCAGAGAGCGCCGGCCCTTGCTGAGCGCTGGTGTGGCGCAAgtgct                    >  2:731612/1‑140 (MQ=255)                                                                                               tctcCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGa                  <  2:450285/140‑1 (MQ=255)                                                                                               tctcCACCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGa                  >  1:692486/1‑140 (MQ=255)                                                                                                    aCCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGgcg                            >  1:914342/1‑125 (MQ=255)                                                                                                    aCCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGgcg                            <  2:914342/125‑1 (MQ=255)                                                                                                    aCCGACGGGTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTg             >  1:754606/1‑140 (MQ=255)                                                                                                          gggTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAg       <  2:395327/140‑1 (MQ=255)                                                                                                           ggTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGc      <  2:754606/140‑1 (MQ=255)                                                                                                           ggTGTTCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGc      >  2:749775/1‑140 (MQ=255)                                                                                                               ttCCGCGCGGAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGTCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGttt  >  2:981309/1‑140 (MQ=255)                                                                                                                       ggAGGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCa           <  1:33650/123‑1 (MQ=255)                                                                                                                          |                                                                                                                                 GGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTT  >  NC_000913/3957363‑3957612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑