Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,961,438	T→C	R254R (CGT→CGC)	rep →	ATP‑dependent DNA helicase Rep

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,961,438	0	T	C	100.0%	335.9 / NA	91	R254R (CGT→CGC)	rep	ATP‑dependent DNA helicase Rep
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/36);  total (55/36)

TATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAA  >  NC_000913/3961304‑3961574                                                                                                                                       |                                                                                                                                         tATCTGCTGGTGGATGCGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCa                                                                                                                                     <  2:31360/140‑1 (MQ=255) tATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCa                                                                                                                                     >  2:634713/1‑140 (MQ=255)     tgctgGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAAc                                                                                                                                 <  1:869375/140‑1 (MQ=255)             gATGAGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGtgct                                                                                                                         >  1:121567/1‑140 (MQ=255)             gATGAGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGtgct                                                                                                                         >  2:705372/1‑140 (MQ=255)               tGAGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGtgctgc                                                                                                                       >  1:833482/1‑140 (MQ=255)               tGAGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGtgctgc                                                                                                                       >  1:359589/1‑140 (MQ=255)               tGAGTATCAGGACACCAACACCAGCC‑GTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTgctgct                                                                                                                      >  1:861936/1‑140 (MQ=255)                 aGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGctgctg                                                                                                                     >  2:606399/1‑140 (MQ=255)                       aGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCCGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAg                                                                                                               >  2:737403/1‑140 (MQ=255)                           caccaacaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGAtt                                                                                                           >  1:126421/1‑140 (MQ=255)                           caccaacaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGAtt                                                                                                           >  2:916258/1‑140 (MQ=255)                           caccaacaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCACAAAACCTGGTGCTGCTGAGTCAGGAtt                                                                                                           >  2:7249/1‑140 (MQ=255)                            accaacaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGAttt                                                                                                          >  2:8722/1‑140 (MQ=255)                             ccaacaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGAtttt                                                                                                         >  1:1043106/1‑140 (MQ=255)                              caacaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTc                                                                                                        >  2:196552/1‑140 (MQ=255)                              caacaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTc                                                                                                        >  2:695440/1‑140 (MQ=255)                               aacaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTcc                                                                                                       <  1:8722/140‑1 (MQ=255)                                acaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTcc                                                                                                       <  2:628168/139‑1 (MQ=255)                                acaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTcc                                                                                                       >  1:628168/1‑139 (MQ=255)                                acaccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCg                                                                                                      >  2:628818/1‑140 (MQ=255)                                 caccaGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCgg                                                                                                     >  1:455927/1‑140 (MQ=255)                                   ccagccagTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGgcg                                                                                                   >  1:295058/1‑140 (MQ=255)                                   ccagccagTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGCCAGGATTTTCCGgcg                                                                                                   >  1:679093/1‑140 (MQ=255)                                     agccagTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCCGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCt                                                                                                 <  1:737403/140‑1 (MQ=255)                                      gccagTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTg                                                                                                >  2:42127/1‑140 (MQ=255)                                      gccagTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTg                                                                                                >  1:180177/1‑140 (MQ=255)                                      gccagTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTg                                                                                                >  2:16908/1‑140 (MQ=255)                                        cagTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGag                                                                                              <  2:679093/140‑2 (MQ=255)                                         agTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAg                                                                                             <  2:879609/140‑1 (MQ=255)                                           tATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGt                                                                                           >  1:251510/1‑140 (MQ=255)                                           tATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGt                                                                                           >  2:55903/1‑140 (MQ=255)                                           tATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGt                                                                                           >  2:27818/1‑140 (MQ=255)                                              gAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGAt                                                                                        <  2:567291/140‑1 (MQ=255)                                              gAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGAt                                                                                        <  1:705372/140‑1 (MQ=255)                                                 cTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGCGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTaa                                                                                     >  1:321935/1‑140 (MQ=255)                                                     tGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCtt                                                                                 >  2:932762/1‑140 (MQ=255)                                                        aaCTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAg                                                                              <  2:121567/140‑1 (MQ=255)                                                        aaCTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAg                                                                              <  2:1043106/140‑1 (MQ=255)                                                         aCTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGc                                                                             >  2:564698/1‑140 (MQ=255)                                                              tggtggGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAAc                                                                        <  1:718248/140‑1 (MQ=255)                                                              tggtggGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAAc                                                                        >  2:718248/1‑140 (MQ=255)                                                                     cAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGct                                                                 >  1:812656/1‑140 (MQ=255)                                                                     cAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGct                                                                 <  2:126421/140‑1 (MQ=255)                                                                       gCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGctct                                                               >  1:813566/1‑140 (MQ=255)                                                                        ccGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCtt                                                              >  2:298861/1‑140 (MQ=255)                                                                        ccGCGCGCGCTTTACCGTGGTGAGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCtt                                                              >  1:626874/1‑140 (MQ=255)                                                                        ccGCGCGCGCTTTACCGTGGCGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCtt                                                              >  1:977002/1‑140 (MQ=255)                                                                            gcgcgcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCgg                                                          <  1:916258/140‑1 (MQ=255)                                                                              gcgcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGc                                                        <  2:455927/140‑1 (MQ=255)                                                                                gcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGt                                                      >  1:464283/1‑140 (MQ=255)                                                                                gcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGt                                                      <  2:321935/140‑1 (MQ=255)                                                                                          ggtgggtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAg                                            <  1:932762/140‑1 (MQ=255)                                                                                          ggtgggtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCACAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAg                                            <  1:7249/140‑1 (MQ=255)                                                                                           gtgggtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgc                                           <  1:16908/140‑1 (MQ=255)                                                                                              ggtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcggc                                        >  1:294486/1‑140 (MQ=255)                                                                                               gtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcggcg                                       <  2:849761/140‑1 (MQ=255)                                                                                               gtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcggcg                                       <  1:42127/140‑1 (MQ=255)                                                                                               gtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcggcg                                       <  2:833482/140‑1 (MQ=255)                                                                                               gtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcggcg                                       <  2:977002/140‑1 (MQ=255)                                                                                               gtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcggcg                                       <  1:634713/140‑1 (MQ=255)                                                                                                tgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGa                                      <  2:258203/140‑1 (MQ=255)                                                                                                   cGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACa                                   <  1:695440/140‑1 (MQ=255)                                                                                                      tGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcgg                                         <  2:83112/131‑1 (MQ=255)                                                                                                      tGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcgg                                         >  1:83112/1‑131 (MQ=255)                                                                                                      tGACCAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATcc                                >  2:23804/1‑140 (MQ=255)                                                                                                          cAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                            <  2:813566/140‑1 (MQ=255)                                                                                                          cAGTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                            <  2:295058/140‑1 (MQ=255)                                                                                                            gTCGATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCg                          >  2:165887/1‑140 (MQ=255)                                                                                                               gATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAGCTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCa                       >  2:986524/1‑140 (MQ=255)                                                                                                               gATCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCa                       >  1:479377/1‑140 (MQ=255)                                                                                                                aTCTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCaa                      >  1:56908/1‑140 (MQ=255)                                                                                                                  cTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATa                    >  2:363634/1‑140 (MQ=255)                                                                                                                  cTACTCCTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATa                    >  1:147457/1‑140 (MQ=255)                                                                                                                       ccTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAAcccc               >  2:571089/1‑139 (MQ=255)                                                                                                                       ccTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCg               >  1:824180/1‑140 (MQ=255)                                                                                                                       ccTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCg               >  2:1029165/1‑140 (MQ=255)                                                                                                                       ccTGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCg               <  1:1029165/140‑1 (MQ=255)                                                                                                                         tGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCa             <  1:55903/140‑1 (MQ=255)                                                                                                                         tGGCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCa             <  2:147457/140‑1 (MQ=255)                                                                                                                          ggCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCAc            >  1:336300/1‑140 (MQ=255)                                                                                                                          ggCGCGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCAc            >  2:82897/1‑140 (MQ=255)                                                                                                                           gcgcGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg           >  1:425726/1‑140 (MQ=255)                                                                                                                           gcgcGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg           >  1:934388/1‑140 (MQ=255)                                                                                                                           gcgcGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg           >  2:126936/1‑140 (MQ=255)                                                                                                                           gcgcGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg           >  2:557810/1‑140 (MQ=255)                                                                                                                           gcgcGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg           >  2:748430/1‑140 (MQ=255)                                                                                                                           gcgcGGTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg           <  1:564698/140‑1 (MQ=255)                                                                                                                                gTGCACGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCttt      >  2:742299/1‑140 (MQ=255)                                                                                                                                    aCGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGaaa  <  2:464283/140‑1 (MQ=255)                                                                                                                                    aCGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGaaa  <  1:742299/140‑1 (MQ=255)                                                                                                                                    aCGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGaaa  <  1:126936/140‑1 (MQ=255)                                                                                                                                    aCGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGaaa  <  1:986524/140‑1 (MQ=255)                                                                                                                                    aCGCCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGaaa  <  1:557810/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAGCTGGTGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAA  >  NC_000913/3961304‑3961574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑