Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	244,714	Δ1 bp	coding (388/768 nt)	yafJ →	putative amidotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	244,714	0	A	.	100.0%	314.5 / NA	73	coding (388/768 nt)	yafJ	putative amidotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (38/35);  total (38/35)

AGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATG  >  NC_000913/244580‑244848                                                                                                                                       |                                                                                                                                       aGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGaaa                                                                                                                                  <  1:621670/140‑1 (MQ=255) aGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGaaa                                                                                                                                  <  1:582539/140‑1 (MQ=255)  ggTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGaaaa                                                                                                                                 >  2:208648/1‑140 (MQ=255)  ggTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGaaaa                                                                                                                                 >  1:182551/1‑140 (MQ=255)       cgcTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCt                                                                                                                            >  1:963530/1‑140 (MQ=255)             aaaaTACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTctgct                                                                                                                      <  1:208648/140‑1 (MQ=255)             aaaaTACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTctgct                                                                                                                      <  2:586027/140‑1 (MQ=255)                  aCTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTc                                                                                                                 <  2:362890/140‑1 (MQ=255)                      aCCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGc                                                                                                             >  1:875829/1‑140 (MQ=255)                           tttACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATaaa                                                                                                        <  2:115664/140‑1 (MQ=255)                                ccGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAAc                                                                                                   <  2:391766/140‑1 (MQ=255)                                 cgcgAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACg                                                                                                  <  2:974763/140‑1 (MQ=255)                                          tGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTAc                                                                                         >  1:438730/1‑140 (MQ=255)                                                cGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCgcgc                                                                                   >  1:699997/1‑140 (MQ=255)                                                cGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCgcgc                                                                                   >  1:640089/1‑140 (MQ=255)                                                 gTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGca                                                                                  <  2:1018880/140‑1 (MQ=255)                                                  tAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGcac                                                                                 >  1:266856/1‑140 (MQ=255)                                                        gACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCggg                                                                           >  1:34486/1‑140 (MQ=255)                                                        gACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCggg                                                                           <  2:1082405/140‑1 (MQ=255)                                                        gACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCggg                                                                           <  2:83734/140‑1 (MQ=255)                                                        gACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCggg                                                                           <  2:875829/140‑1 (MQ=255)                                                             aTGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACa                                                                      >  1:800233/1‑140 (MQ=255)                                                             aTGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACa                                                                      >  2:24486/1‑140 (MQ=255)                                                              tGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACAt                                                                     >  2:11084/1‑140 (MQ=255)                                                               gCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGcaca                                                                                >  1:584400/1‑128 (MQ=255)                                                               gCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGcaca                                                                                <  2:584400/128‑1 (MQ=255)                                                                cccATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATgg                                                                   <  2:359560/140‑1 (MQ=255)                                                                cccATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATgg                                                                   >  2:130015/1‑140 (MQ=255)                                                                cccATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCCGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATgg                                                                   >  1:922377/1‑140 (MQ=255)                                                                   aTAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATggcgg                                                                <  1:357445/140‑1 (MQ=255)                                                                   aTAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATggcgg                                                                >  1:671901/1‑140 (MQ=255)                                                                   aTAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATggcgg                                                                <  1:682216/140‑1 (MQ=255)                                                                   aTAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATggcgg                                                                >  1:38388/1‑140 (MQ=255)                                                                     aaCGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGgcggcg                                                              <  2:38388/140‑1 (MQ=255)                                                                     aaCGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGgcggcg                                                              <  2:182551/140‑1 (MQ=255)                                                                     aaCGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGgcggcg                                                              >  1:966100/1‑140 (MQ=255)                                                                     aaCGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGgcggcg                                                              >  1:19019/1‑140 (MQ=255)                                                                      aCGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGcggcgg                                                             <  1:160732/140‑1 (MQ=255)                                                                      aCGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGcggcgg                                                             <  2:800233/140‑1 (MQ=255)                                                                       cGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGt                                                            >  1:388264/1‑140 (MQ=255)                                                                        ggACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTa                                                           <  1:261351/140‑1 (MQ=255)                                                                        ggACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTa                                                           <  1:24486/140‑1 (MQ=255)                                                                        ggACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTa                                                           <  2:34486/140‑1 (MQ=255)                                                                         gACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGTAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTAt                                                          >  2:952603/1‑140 (MQ=255)                                                                         gACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTAt                                                          <  2:966100/140‑1 (MQ=255)                                                                         gACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTAt                                                          <  2:19019/140‑1 (MQ=255)                                                                          aCAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTAtt                                                         >  1:261095/1‑140 (MQ=255)                                                                          aCAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTAtt                                                         >  1:756974/1‑140 (MQ=255)                                                                           cAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTAttt                                                        <  1:519343/140‑1 (MQ=255)                                                                              cTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTaaa                                                     >  1:963628/1‑140 (MQ=255)                                                                               tGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAat                                                    >  1:756347/1‑140 (MQ=255)                                                                                  cGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATggcgg                                                                <  1:116870/125‑1 (MQ=255)                                                                                  cGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATggcgg                                                                >  2:116870/1‑125 (MQ=255)                                                                                   gggCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAata                                                   <  2:481050/137‑1 (MQ=255)                                                                                   gggCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAata                                                   >  1:481050/1‑137 (MQ=255)                                                                                      cTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGc                                             >  1:145225/1‑140 (MQ=255)                                                                                      cTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGc                                             >  2:791500/1‑140 (MQ=255)                                                                                        aCAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCt                                           <  1:11084/140‑1 (MQ=255)                                                                                         cAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTc                                          >  1:922214/1‑140 (MQ=255)                                                                                            aTCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACt                                       <  2:756347/140‑1 (MQ=255)                                                                                            aTCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACt                                       <  1:130015/140‑1 (MQ=255)                                                                                                 tGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCgg                                  >  1:538596/1‑140 (MQ=255)                                                                                                  ggAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGa                                 <  2:671901/140‑1 (MQ=255)                                                                                                      aCCGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGaa                             >  1:140904/1‑140 (MQ=255)                                                                                                        cGGCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACt                           >  2:922561/1‑140 (MQ=255)                                                                                                         ggCAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTg                          <  2:832349/140‑1 (MQ=255)                                                                                                           cAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCg                        >  1:652743/1‑140 (MQ=255)                                                                                                           cAACTTCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCg                        <  2:963530/140‑1 (MQ=255)                                                                                                                tCCGCCCGGTAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGa                   >  2:201336/1‑140 (MQ=255)                                                                                                                         tAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGttt          >  2:288141/1‑140 (MQ=255)                                                                                                                         tAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGttt          >  2:844112/1‑140 (MQ=255)                                                                                                                         tAGGCGAAACCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGttt          >  2:414788/1‑140 (MQ=255)                                                                                                                                 aCCGAC‑GCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACAtg  <  2:384437/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATG  >  NC_000913/244580‑244848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑